Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4730CT100.0% 136.3 / ‑6.5 47A67T (GCT→ACT) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/18);  minor base G (0/1);  total (28/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATAT  >  CP000730/1744337‑1744607
                                                                                                                                        |                                                                                                                                      
tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAATCATTTGTACCATATTCTAGGTCTTACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTCCAAAGCCATTCCCCATTGACTAAGTTAcc                                                                                                                                    >  1:564334/1‑140 (MQ=255)
   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTCGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCGTAGCCAATCCCGATTGACTAATTTACAGGa                                                                                                                                 >  1:261973/1‑141 (MQ=255)
   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGa                                                                                                                                 <  1:154492/141‑1 (MQ=255)
    cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGAc                                                                                                                                <  1:100696/141‑1 (MQ=255)
     tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACa                                                                                                                               >  1:248699/1‑141 (MQ=255)
       gTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGc                                                                                                                             <  2:56258/141‑1 (MQ=255)
                       ttgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcg                                                                                                             <  2:519911/140‑1 (MQ=255)
                          cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                          <  1:530976/141‑1 (MQ=255)
                                    aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCt                                                                                                >  1:204689/1‑141 (MQ=255)
                                    aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTCAATAAGTTACAGGACAGCCAATGATAAAGCCACCCGCATCCGCTccc                                                                                                >  2:657973/1‑140 (MQ=255)
                                       tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa                                                                                             >  1:638651/1‑141 (MQ=255)
                                        ttGTACTATATTCTAGGGCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                                            <  2:59164/141‑1 (MQ=255)
                                         tGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAAc                                                                                           >  2:667278/1‑141 (MQ=255)
                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa                                                                                         >  2:395256/1‑141 (MQ=255)
                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCACTGATAAAGCCACGCGCTTCAGGTCCTACCACGa                                                                                         >  2:35116/1‑141 (MQ=255)
                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGCCCTACAACGa                                                                                         >  2:549543/1‑141 (MQ=255)
                                             ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCTACAACGata                                                                                       >  2:336142/1‑141 (MQ=255)
                                                   tCTAGGTCATACTCATAACGAATGACTTCGCGAGGTAATTTCCATCCTGTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACGGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAGAGATATCaaaa                                                                                 >  2:595747/1‑139 (MQ=255)
                                                      aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGACAATGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                              <  2:456795/141‑1 (MQ=255)
                                                       ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                                             >  2:569212/1‑141 (MQ=255)
                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:198658/1‑141 (MQ=255)
                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:658517/1‑141 (MQ=255)
                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:462590/1‑141 (MQ=255)
                                                                       aaTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTc                                                             <  2:638651/141‑1 (MQ=255)
                                                                        aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCACAGCCAATCCCCATTGGATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACATCGATATCAACATCTCTGTCTTTTGAGTATTcc                                                            >  1:610805/1‑140 (MQ=255)
                                                                          gACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTaa                                                          >  1:192297/1‑140 (MQ=255)
                                                                             ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGCTATCAACATCTCGGTCATTTGCGTATTCTCCAAt                                                       >  1:81634/1‑141 (MQ=255)
                                                                               cACGAGGTAATTTCCCTTCTTTTCTAACAGGGGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCGATAACGATACCAACACCACCGTCTTTTGCGTATTCtgaaaatt                                                     <  2:496493/141‑8 (MQ=255)
                                                                                     gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCtt                                               >  2:1897/1‑140 (MQ=255)
                                                                                      tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                                              <  2:356617/141‑1 (MQ=255)
                                                                                       aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                             <  1:581156/141‑1 (MQ=255)
                                                                                            cccTTCTTTTCTCACAGGTGCAAAGCCAATCCCCATTGAGTAAGTAACCGGACAGCCAATGATAAAGCCACGCCGTTCGGGTCCTACAACGATATCAACATCTCTGACTTCTGCGTATTCTAACATTTTATCTGTTGCATa                                        >  2:265865/1‑141 (MQ=255)
                                                                                            cccTTCTTTTCTAACAGGTGCGAGCTTAATTCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATa                                        >  1:686825/1‑141 (MQ=255)
                                                                                                  ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                                  <  1:97989/141‑1 (MQ=255)
                                                                                                   tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat                                 >  1:587583/1‑141 (MQ=255)
                                                                                                    ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTCACCGGAGTGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATg                                <  1:190722/141‑1 (MQ=255)
                                                                                                      cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCCTTTGCt                              >  2:38058/1‑141 (MQ=255)
                                                                                                          cagtcgcgaaGCCAATCCCCATGGAATAAGGTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc                          <  2:610805/133‑1 (MQ=255)
                                                                                                           aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTTCATAGCCATATGCTTCAcc                         >  1:555824/1‑141 (MQ=255)
                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:482966/1‑141 (MQ=255)
                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:531103/1‑141 (MQ=255)
                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATATGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:35488/1‑141 (MQ=255)
                                                                                                                    cccAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt                <  1:110208/140‑1 (MQ=255)
                                                                                                                      cAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa              <  1:403942/141‑1 (MQ=255)
                                                                                                                         tCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTg           <  1:569212/141‑1 (MQ=255)
                                                                                                                           cccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta         <  1:438770/141‑1 (MQ=255)
                                                                                                                                  aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAatat  <  1:395256/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATAT  >  CP000730/1744337‑1744607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: