Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,746,458 T→C 6.3% E170G (GAA→GGA)  recJ ← recombination protein RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,746,4580TC 6.3% 230.9 / 4.4 80E170G (GAA→GGA) recJrecombination protein RecJ
Reads supporting (aligned to +/- strand):  ref base T (33/41);  new base C (2/3);  total (35/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

TAATCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTAGTTCGTTTGG  >  CP000730/1746320‑1746597
                                                                                                                                          |                                                                                                                                           
taatCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCa                                                                                                                                           >  2:438527/1‑141 (MQ=255)
  atCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCatg                                                                                                                                         >  2:439429/1‑141 (MQ=255)
  atCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCatg                                                                                                                                         >  2:417484/1‑141 (MQ=255)
  atCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCatg                                                                                                                                         <  1:391136/141‑1 (MQ=255)
  aacAGGTACATTTTCAATCAATGCTTGTGCTAATCTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCatg                                                                                                                                         <  1:227546/139‑1 (MQ=255)
      ggTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCAtgatga                                                                                                                                     <  2:413159/141‑1 (MQ=255)
          cATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAg                                                                                                                                 <  1:104609/141‑1 (MQ=255)
           aTTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGt                                                                                                                                <  1:73168/141‑1 (MQ=255)
            ttttCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGtt                                                                                                                               >  1:371411/1‑141 (MQ=255)
              ttCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTAc                                                                                                                             <  1:530973/141‑1 (MQ=255)
               tcaagcaaTGCTTGTGCTAATTTGTATGCAACACCTGCAGCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACa                                                                                                                            <  2:322850/141‑1 (MQ=255)
                caatcaatGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCGGGTAATGTACTACCAATTTCATGATGATCAGTTACaa                                                                                                                           >  1:190615/1‑141 (MQ=255)
                 aatcaatTCGTGTGCTGATTTGGATGACACCCCTGCGCCACTCAATTGTTGGAACGGATAATTCAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAAt                                                                                                                          <  2:431413/141‑1 (MQ=255)
                    ccatGCTTGTTCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  1:644299/139‑1 (MQ=255)
                    caatGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  2:183226/141‑1 (MQ=255)
                    caatGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  2:85973/141‑1 (MQ=255)
                    caatGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  1:545037/141‑1 (MQ=255)
                    caatGCTTGGGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  2:672525/141‑1 (MQ=255)
                    caatGCGTGTGCTAATTTGGATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGAc                                                                                                                       <  2:661710/141‑1 (MQ=255)
                     aatGCGTGTGCTAATTTGGATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATCTCCGGTTTCAGACGCTGCAGGTACTGTGCTACCAATTTCATGATGATCAGTTACAATGACa                                                                                                                      <  2:146931/141‑1 (MQ=255)
                            gtgCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTc                                                                                                               <  1:417484/141‑1 (MQ=255)
                               cTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTa                                                                                                            <  2:222838/141‑1 (MQ=255)
                                tAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTaa                                                                                                           <  1:202175/141‑1 (MQ=255)
                                    ttGTATGCAACACCTGCACCACACAATTGTTGGAATGGATAATGAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCt                                                                                                       <  1:360794/141‑1 (MQ=255)
                                      gTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTg                                                                                                     >  2:658502/1‑141 (MQ=255)
                                      gTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTg                                                                                                     >  2:98786/1‑141 (MQ=255)
                                            aaCACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAACTCTTGAACCAt                                                                                               >  1:13339/1‑141 (MQ=255)
                                            aaCACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAAAGACCTCAAATCCTACATCTTAGACCAt                                                                                               >  2:281177/1‑141 (MQ=255)
                                              cacCAGCACCACACCATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATCTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCAttt                                                                                             <  1:577003/141‑1 (MQ=255)
                                               acCTGCACCACACAATTGGTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCAtttt                                                                                            <  2:535388/141‑1 (MQ=255)
                                                   gCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTGACTCTTGAACCATGTTAAtt                                                                                        >  1:246482/1‑141 (MQ=255)
                                                   gCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAtt                                                                                        <  1:619311/141‑1 (MQ=255)
                                                   gCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAtt                                                                                        >  1:52127/1‑141 (MQ=255)
                                                   gCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAtt                                                                                        >  2:257306/1‑141 (MQ=255)
                                                    caccacACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTCCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAttt                                                                                       >  1:60495/1‑141 (MQ=255)
                                                    caccacACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGAATGCATATGCTTCTGGTAATGTACTACCAATTCCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAttt                                                                                       >  2:455669/1‑141 (MQ=255)
                                                    caacacacaATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAAttt                                                                                       <  1:291867/138‑1 (MQ=255)
                                                     cccacACAATTGTGGAAACGTATAATTAAACGCTGGATGCATAGGATGTACGATTGCATATGCTTCTGGTAATGTCCTCCCCATTTCATGATGATCAGTTACATTGACATCAACTCCTAAATCTTGAACCATTTTCATTTc                                                                                      <  2:609047/140‑1 (MQ=255)
                                                     accaccCAATTATTGGAACGGATAATTAAACGATGGATTCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTc                                                                                      <  2:151885/141‑1 (MQ=255)
                                                     accacACAATTGGTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTc                                                                                      <  2:322366/141‑1 (MQ=255)
                                                         cagaaTTTTTGGAACGGATAATTAAACGGTGGATGCATTGGATGCACGATTGCATATGTTTCTGGTAATGTACTACCAATTTCATGCTGATCATTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTa                                                                                  <  2:291238/138‑1 (MQ=255)
                                                           cgaTTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTTCGATTGCATATGCTTTTGGTAATGTACTACCAATTTCATGTGGATCAGTTACAACGACATCACCTCCTACATCCTGAACCATTTTTATTTCATTacc                                                                                <  2:580094/139‑3 (MQ=255)
                                                           caATTGTTAGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAAGTTCATGATGATCACTTACATTGTCATCAACTCCTAAATCCTGAACCATTTTAATTTccgcatg                                                                                >  2:71255/1‑135 (MQ=255)
                                                                 ttgGAACGGATAATTAAACGATGGATGCGTTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCttt                                                                          >  1:441684/1‑140 (MQ=255)
                                                                  tgGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGCCCgtgt                                                                         >  1:53025/1‑141 (MQ=255)
                                                                       cggtttaTTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGcc                                                                    <  2:225215/135‑1 (MQ=255)
                                                                       cGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGcc                                                                    <  1:87418/141‑1 (MQ=255)
                                                                          aTAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCCACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGtctagcatt                                                                 >  2:448530/1‑133 (MQ=255)
                                                                             aTTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATtctc                                                              >  2:37861/1‑141 (MQ=255)
                                                                                      tggatgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTAt                                                     >  2:48949/1‑141 (MQ=255)
                                                                                       ggatgCATTGGATGTTCGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGACCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATg                                                    <  2:204103/141‑1 (MQ=255)
                                                                                       ggatgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATg                                                    >  1:32550/1‑141 (MQ=255)
                                                                                       ggatgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTAATTATGACCTTGTCTGCCATTATGACCATTTAta                                                    >  1:121011/1‑140 (MQ=255)
                                                                                        gatgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATATTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTTTCAACAGTTATGa                                                   >  2:381886/1‑141 (MQ=255)
                                                                                          tgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGGACTTGTATGCCATTATCAACAGTTTTGAtt                                                 >  2:118372/1‑141 (MQ=255)
                                                                                          tgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTTTATGCCATTATCAACAGTTattatt                                                 >  1:576719/1‑141 (MQ=255)
                                                                                          tgCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGAtt                                                 >  1:154728/1‑141 (MQ=255)
                                                                                              ttGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATg                                             <  2:145562/141‑1 (MQ=255)
                                                                                               tGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGt                                            <  2:32550/141‑1 (MQ=255)
                                                                                                ggATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGtt                                           >  1:560685/1‑141 (MQ=255)
                                                                                                   tGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATg                                        >  1:257020/1‑141 (MQ=255)
                                                                                                       cGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCtt                                    >  1:10550/1‑141 (MQ=255)
                                                                                                        gATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATAAACACTTATGATTAATGTTATGCCTtc                                   <  2:509896/141‑1 (MQ=255)
                                                                                                         agtGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGGTATGATTAATGTTATGCCTtca                                  >  2:98917/3‑141 (MQ=255)
                                                                                                         aTTGCTTATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTtca                                  <  1:302058/141‑1 (MQ=255)
                                                                                                          ttGCATATGCTTCTGGTAATGTACTACCAATTCCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTtcat                                 <  1:496344/141‑1 (MQ=255)
                                                                                                          ttGCATATGCTTCTGGTAATGTACTACCAATTCCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTtcat                                 <  1:455669/141‑1 (MQ=255)
                                                                                                          ttGCATATGCTTCTGGTAATGTACTACCAATTCCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTtcat                                 <  2:60495/141‑1 (MQ=255)
                                                                                                            gCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGGTATGCCTtcatca                               <  1:482040/141‑1 (MQ=255)
                                                                                                               tatGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGa                            >  2:288989/1‑141 (MQ=255)
                                                                                                               tatGCTTCTGGTAAGTTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTCTGCCCCTGTATGGCGTGATGAAGCGTTATGATTAATGTTATGCCTTCATCATGa                            >  2:662681/1‑141 (MQ=255)
                                                                                                                ttGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGGTTAATGTTATGCCTTCATCATGAg                           <  1:670857/140‑1 (MQ=255)
                                                                                                                 tgtttCCGGTAATGTGCTCCCAATTTCAGCATGATCANGTACAATGACATCACCTCCTTTATCGTGAACCATTTTATTTTTAGTATGACCTTGCATGCCATTATTAGCAGTTATTCTTAATGTTATGCCTTCATCATGAGc                          <  2:126512/138‑1 (MQ=255)
                                                                                                                 tGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGc                          >  2:258310/1‑141 (MQ=255)
                                                                                                                 tGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATAAACAGTTATGATTAATGTTATGCCTTCATCATGaac                          >  2:281996/1‑139 (MQ=255)
                                                                                                                  gCTTCTGGTAATGGTCTACCAATTTCATGATGATCAGGTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCa                         <  2:441684/141‑1 (MQ=255)
                                                                                                                    ttCTGTTAGTGTACTACCAATTGTATGATGATCAGTTACAAGGACATCAACTCCTAAATCTTTAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCAtt                       <  2:524136/141‑1 (MQ=255)
                                                                                                                                 cttccAATTTCATGATGATCAGTTACAAGGACAGCAATTACTACCGAAAGTTCCGTTTTAATTTCATTATTACCTCGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTAGt          <  1:328847/138‑1 (MQ=255)
                                                                                                                                 ctacCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTAGt          >  1:674818/1‑141 (MQ=255)
                                                                                                                                 ctacCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTAGt          <  2:190615/141‑1 (MQ=255)
                                                                                                                                         ttCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACATTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTACGTCGTTTgg  >  2:378114/1‑141 (MQ=255)
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TAATCAGGTACATTTTCAATCAATGCTTGTGCTAATTTGTATGCAACACCTGCACCACACAATTGTTGGAACGGATAATTAAACGATGGATGCATTGGATGTACGATTGCATATGCTTCTGGTAATGTACTACCAATTTCATGATGATCAGTTACAATGACATCAACTCCTAAATCTTGAACCATTTTAATTTCATTATGACCTTGTATGCCATTATCAACAGTTATGATTAATGTTATGCCTTCATCATGAGCATTTCTAAATGCTAGTTCGTTTGG  >  CP000730/1746320‑1746597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: