Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 238,030 T→C 5.7% L11L (TTA→CTA)  USA300HOU_0214 → oligopeptide ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730238,0300TC 5.7% 201.5 / 2.5 70L11L (TTA→CTA) USA300HOU_0214oligopeptide ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (44/22);  new base C (2/2);  total (46/24)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

TCGTATATATATCAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATT  >  CP000730/237891‑238170
                                                                                                                                           |                                                                                                                                            
tCGTATATATATCAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCtt                                                                                                                                             <  1:211052/141‑1 (MQ=255)
     tatatatCAATTGTAATATCTTTAGCAAATTTAATTATTGATATATGTTATGCTTTAATCGATCCAAGATGACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAat                                                                                                                                        <  2:245013/141‑1 (MQ=255)
            cAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGc                                                                                                                                 <  1:368720/141‑1 (MQ=255)
                tGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGt                                                                                                                             >  1:574670/1‑141 (MQ=255)
                 gTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCGAAAATTACGTAGTGAAATCAACGAAAGGACGCGCAGCTGTGGGTACAACTTAATTCAAAGATAGCTTGCTTAAAATTATTCGAAAGtt                                                                                                                            >  1:19694/1‑141 (MQ=255)
                 gTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTGTTCGCAAGtt                                                                                                                            >  1:119023/1‑141 (MQ=255)
                 gTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGtt                                                                                                                            <  1:27421/141‑1 (MQ=255)
                 gTAAGATCTTTAGCAAACTTAATTATTGATATATGTTAAGCTGTAATCGATCAAAAATTAAGAAGGGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACGTAGTTCAAAGCTCGCTTCATAAAAATCATACGAAAGtt                                                                                                                            >  2:376062/1‑141 (MQ=255)
                  tAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATTAACGAAAGGAAGTAAGGCATATGGCACAACTTAATTCAAAGATAGCTTCTTTAAAATTATTCGCAAGATa                                                                                                                           >  2:805/1‑141 (MQ=255)
                  tAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGttt                                                                                                                           >  2:57627/1‑140 (MQ=255)
                  tAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTa                                                                                                                           >  2:219478/1‑141 (MQ=255)
                  tAATATCTTTAGCAAACTTAATTATTGATATATTATATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGTAAGTGAGGCATATGGCACAACTTAATTCAAAGATCGCTTCCTTAAAATTATTCGCCAGtca                                                                                                                           >  2:140842/1‑139 (MQ=255)
                   aaTATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTCCCTTAAAATTATTCGCAAGTTAc                                                                                                                          <  1:407811/141‑1 (MQ=255)
                    atatCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACg                                                                                                                         >  1:301970/1‑141 (MQ=255)
                      atCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGcc                                                                                                                       >  1:225655/1‑141 (MQ=255)
                      atCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGcc                                                                                                                       >  2:475469/1‑141 (MQ=255)
                            aGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCa                                                                                                                 >  2:81845/1‑141 (MQ=255)
                              cAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAAc                                                                                                               >  2:288714/1‑141 (MQ=255)
                                 aCTTAATTATTGATATATTTTATGCTTTAATCGCTCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCTCAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTta                                                                                                            <  2:387642/141‑1 (MQ=255)
                                 aCTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACtaa                                                                                                            >  1:571590/1‑139 (MQ=255)
                                 aCTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTta                                                                                                            >  1:399252/1‑141 (MQ=255)
                                 aCTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCAATAGCAACTta                                                                                                            >  2:586301/1‑141 (MQ=255)
                                   tttattattGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTtata                                                                                                          <  2:587697/138‑1 (MQ=255)
                                   ttAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCACCTcata                                                                                                          >  2:608981/1‑141 (MQ=255)
                                      attattGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATAttt                                                                                                       >  2:311566/1‑141 (MQ=255)
                                      attattGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCCTAAAATTATTCGCAAGTTACGCCATAGCAACTTATAttt                                                                                                       >  2:535686/1‑141 (MQ=255)
                                      attattGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCCTAAAATTATTCGCAAGTTACGCCATAGCAACTTATAttt                                                                                                       >  2:267487/1‑141 (MQ=255)
                                              tatatTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTTTATTTTAGTtata                                                                                               >  2:403543/1‑141 (MQ=255)
                                              tatatTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTtata                                                                                               >  2:331548/1‑141 (MQ=255)
                                                  ttttATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTaa                                                                                           >  2:555188/1‑141 (MQ=255)
                                                      aTGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAg                                                                                       >  2:256727/1‑141 (MQ=255)
                                                      aTGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAg                                                                                       >  2:642801/1‑141 (MQ=255)
                                                      aTGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCATCCTTAAAATTATTCCAAAGGTACACCATAGCAACTTATTTTTTAGTTATATTAATGAg                                                                                       >  2:230939/1‑141 (MQ=255)
                                                                cGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAAt                                                                             <  2:32326/141‑1 (MQ=255)
                                                                 gATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATc                                                                            <  1:309987/141‑1 (MQ=255)
                                                                     cAAAATTACATAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCtttt                                                                        <  2:542785/141‑1 (MQ=255)
                                                                         aTTACGTCCTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTaaa                                                                    >  2:426171/1‑141 (MQ=255)
                                                                          ttACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAg                                                                   >  2:645968/1‑141 (MQ=255)
                                                                              gTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAGATCTTTTTAAAGGTTa                                                               >  1:247014/1‑141 (MQ=255)
                                                                               tAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACACCATAGCAACt                                                                                                              >  2:112279/1‑93 (MQ=255)
                                                                               tAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACACCATAGCAACt                                                                                                              <  1:112279/93‑1 (MQ=255)
                                                                               tAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCCTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTAt                                                              <  1:267487/141‑1 (MQ=255)
                                                                               tAGTGAAATCAACGAAAGGAAGTGAGGCATACGGCACAACTTAATTCAAAGATAGCTTCCCTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTAt                                                              <  1:535686/141‑1 (MQ=255)
                                                                                 gTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTAtgt                                                            <  1:171232/141‑1 (MQ=255)
                                                                                 gTGAAATCAACGAAAGGAAGTGAAGCATAGCGCTTAATTTTGTTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTAGATTAACGAGTGCATTAAATCTTTTTAAAGGTTAtgt                                                            <  1:91704/141‑1 (MQ=255)
                                                                                   gAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTgg                                                          <  1:174248/141‑1 (MQ=255)
                                                                                     aaTCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGcc                                                        <  1:288714/141‑1 (MQ=255)
                                                                                       ccAACGAAAGGAAGTGAGGCATATGGCAGAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGa                                                      <  1:660222/140‑1 (MQ=255)
                                                                                       acAAAGAAAGGAAGTCAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGa                                                      <  1:10120/140‑1 (MQ=255)
                                                                                        cAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAGAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGAt                                                     <  2:400721/141‑1 (MQ=255)
                                                                                        cAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGAt                                                     <  1:149890/141‑1 (MQ=255)
                                                                                          aCGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATAc                                                   >  1:559206/1‑141 (MQ=255)
                                                                                           cGAAAGGAAGTGAGGCCTATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACg                                                  >  1:348441/1‑141 (MQ=255)
                                                                                           cGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACg                                                  >  1:369420/1‑141 (MQ=255)
                                                                                           cGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACg                                                  >  1:256653/1‑141 (MQ=255)
                                                                                           cGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAAAGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACg                                                  >  2:371342/1‑141 (MQ=255)
                                                                                                      gAGGCAAATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGc                                       <  2:497774/141‑1 (MQ=255)
                                                                                                         gCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATGTTTTATTTATATTAACGAGTGCATTAAGTCTTTTTAAAGGTTATGTGTCCGATACGTTCTATATTGCTGa                                    >  1:43821/1‑141 (MQ=255)
                                                                                                         gCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGa                                    >  2:24580/1‑141 (MQ=255)
                                                                                                                 cacaACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGc                            <  2:179030/141‑1 (MQ=255)
                                                                                                                     aCTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAAt                        >  2:584587/1‑141 (MQ=255)
                                                                                                                        tAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGt                     >  2:515194/1‑141 (MQ=255)
                                                                                                                        tAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGt                     >  2:336989/1‑141 (MQ=255)
                                                                                                                          aTTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGttt                   >  1:20296/1‑141 (MQ=255)
                                                                                                                          aTTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGttt                   >  1:638056/1‑141 (MQ=255)
                                                                                                                          aTTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGttt                   >  2:614630/1‑141 (MQ=255)
                                                                                                                             cAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTATTTTTTATTTGTCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTaa                >  2:608706/1‑141 (MQ=255)
                                                                                                                             cAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTaa                >  2:236580/1‑141 (MQ=255)
                                                                                                                                aGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACca             <  1:81845/141‑1 (MQ=255)
                                                                                                                                  attggttCCTTAAAATTATTGTAAATTGACGCCATAGCAACTTATATTTCAATTATATTAACGGGGGCTTTAAAGTTTTCTGCAGGTTATGATGCCGAAACGGTCGATATTGCTGAAATATGGCGAATCGTCTTAACcatc           <  2:26140/136‑1 (MQ=255)
                                                                                                                                       ttCCTTAAAATTATTCGCAAGTAACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGGTTTAACCATCAtttt      >  2:506399/1‑141 (MQ=255)
                                                                                                                                           ttAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAatt  >  1:543035/1‑141 (MQ=255)
                                                                                                                                           ttAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAatt  >  1:644440/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCGTATATATATCAATTGTAATATCTTTAGCAAACTTAATTATTGATATATTTTATGCTTTAATCGATCCAAAATTACGTAGTGAAATCAACGAAAGGAAGTGAGGCATATGGCACAACTTAATTCAAAGATAGCTTCCTTAAAATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATT  >  CP000730/237891‑238170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: