Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,232,148 G→T G18V (GGC→GTC)  prkC → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,1480GT98.6% 211.4 / NA 71G18V (GGC→GTC) prkCnon‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (42/28);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA  >  CP000730/1232010‑1232284
                                                                                                                                          |                                                                                                                                        
ggTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGgcgtcg                                                                                                                                        >  1:260035/1‑141 (MQ=255)
ggTGATCAATTAATGCAATTGGCATTAGATAACCATTAGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTGTGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCGTGgcgtcg                                                                                                                                        >  1:152677/1‑141 (MQ=255)
               cAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCg                                                                                                                         <  2:21350/141‑1 (MQ=255)
               cAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCg                                                                                                                         <  2:708240/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAGCGTTACTGTCACACTCGCGTTTATTGACTGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGACAAGCTTGGCGTCGGTGGCATGAGTACCGt                                                                                                                        <  2:758219/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCTGCGGTGGCATGAGTACCGt                                                                                                                        <  1:528791/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt                                                                                                                        <  2:854836/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt                                                                                                                        <  2:670864/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGt                                                                                                                        <  1:93690/141‑1 (MQ=255)
                aaTTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGCCTATTGAAGGTGCTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGAGGCATGAGTACCGt                                                                                                                        <  2:679542/141‑1 (MQ=255)
                aaTGGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATCAGCTTTGCGTCGGTGGCATGAGTACCGt                                                                                                                        <  2:960992/141‑1 (MQ=255)
                  ttGGCATTAGATAACCATTCGAAAGAAAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGttt                                                                                                                      <  1:998621/141‑1 (MQ=255)
                   tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTCTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAAATAAGATTGGCGTCGGTGGCATGAGTACCGTTTa                                                                                                                     >  1:9331/1‑141 (MQ=255)
                   tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGAATAAGAACCGTTTa                                                                                                                     >  1:522187/1‑141 (MQ=255)
                   tGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTAGGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGACATGAGTACCGTTTa                                                                                                                     >  1:9432/1‑141 (MQ=255)
                     gCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATc                                                                                                                   >  1:881286/1‑141 (MQ=255)
                      cATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCt                                                                                                                  <  1:872244/141‑1 (MQ=255)
                        ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTGTCTTg                                                                                                                >  1:14325/1‑141 (MQ=255)
                        ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTg                                                                                                                >  1:941017/1‑141 (MQ=255)
                        ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGGGGCAGGAGTACCGTTTATCTTg                                                                                                                >  1:437815/1‑141 (MQ=255)
                            aTAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGGTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGa                                                                                                            >  1:968573/1‑141 (MQ=255)
                            aTAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGa                                                                                                            >  1:943958/1‑141 (MQ=255)
                                    tCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga                                                                                                    >  1:220055/1‑141 (MQ=255)
                                           aTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTaa                                                                                             >  1:939625/1‑141 (MQ=255)
                                               cGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAtt                                                                                         >  1:913998/1‑141 (MQ=255)
                                                 ttACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATCCGATACTTAACATTaa                                                                                       >  2:427869/1‑141 (MQ=255)
                                                         aTACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGaaa                                                                               >  1:330224/1‑141 (MQ=255)
                                                         aTACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTACTGAAGATACGATACTTAACATTAAAGTTGaaa                                                                               >  2:815328/1‑141 (MQ=255)
                                                            cTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTa                                                                            >  1:355042/1‑141 (MQ=255)
                                                            cTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTa                                                                            <  2:822916/141‑1 (MQ=255)
                                                              cgcgGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAg                                                                          >  1:206396/1‑141 (MQ=255)
                                                                     aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAGGGCGAttt                                                                   <  1:812024/141‑1 (MQ=255)
                                                                     aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttt                                                                   <  1:812061/141‑1 (MQ=255)
                                                                     aTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCGTGGCGTGGGAGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtgt                                                                   >  2:852973/1‑139 (MQ=255)
                                                                       tGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGCAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttttt                                                                 <  2:141882/141‑1 (MQ=255)
                                                                       tGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAttttt                                                                 <  2:409179/141‑1 (MQ=255)
                                                                          aGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTtata                                                              <  2:984753/141‑1 (MQ=255)
                                                                            gTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaca                                                            >  1:720774/1‑140 (MQ=255)
                                                                              gATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccac                                                          >  1:292765/1‑141 (MQ=255)
                                                                              gATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccac                                                          >  1:216715/1‑141 (MQ=255)
                                                                               aTAAAGTATGGTAGGTAAAATAATATATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccacc                                                         <  2:921663/141‑1 (MQ=255)
                                                                               aTAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATaccacc                                                         <  1:887831/141‑1 (MQ=255)
                                                                               aTAAAGTATGATAGGTAAAATAATAAATAAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTAGCAATTAAGGCGCTTTTTATaccacc                                                         >  1:630828/1‑141 (MQ=255)
                                                                                tAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCt                                                        <  1:791537/141‑1 (MQ=255)
                                                                                tAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCAACt                                                        >  2:150908/1‑141 (MQ=255)
                                                                                 aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTCACATTAAAGCTGCAATTAAGGCGATTTTTATACCACCTa                                                       >  2:534355/1‑141 (MQ=255)
                                                                                 aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATGAAGGCGATTTTTATACAAGCTa                                                       >  2:930141/1‑141 (MQ=255)
                                                                                   aGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTaga                                                     >  1:253924/1‑141 (MQ=255)
                                                                                    gTATGATAGGTAAAATAATAAATGAACGAGATAGAATTGTAGATAAGCTTGGCATCGGTGAAATGAGTACCGTGTATCTTGGTGAAGAGACGATACTTAACATTAAAGTGTCAAATAAGGCGAATGTAATACAACCttgga                                                    >  2:537628/1‑137 (MQ=255)
                                                                                       tGATAGGTAAAATAATAAATGAACGATATAAAATGGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGACGATTTTTATACCACCTAGAGaaa                                                 >  1:3794/1‑141 (MQ=255)
                                                                                        taTAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGaaaa                                                <  2:910485/140‑1 (MQ=255)
                                                                                             gTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATATTTATCCCACCTAGGAAAAaaaaag                                           >  2:241520/1‑141 (MQ=255)
                                                                                                   taataaATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACAt                                     <  1:804619/141‑1 (MQ=255)
                                                                                                    aataaATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACAtt                                    <  2:990867/141‑1 (MQ=255)
                                                                                                       aaaTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACGTAGAGAAAAAGAAGAAACATTaaa                                 <  1:724973/141‑1 (MQ=255)
                                                                                                       aaaTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTaaa                                 <  1:840921/141‑1 (MQ=255)
                                                                                                         aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc                               >  1:586576/1‑141 (MQ=255)
                                                                                                         aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGCTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc                               >  1:294873/1‑141 (MQ=255)
                                                                                                              cGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGCGGAGAAGAAGAAACATTAAAACGtttt                          >  2:870201/1‑141 (MQ=255)
                                                                                                                atatAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGa                        >  2:331746/1‑141 (MQ=255)
                                                                                                                atatAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGa                        >  1:527869/1‑141 (MQ=255)
                                                                                                                atatAAAATTGTAGATAAGCTTGGCGTCGGTCGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAGCGttttta                        >  2:767345/1‑139 (MQ=255)
                                                                                                                    aaaaTTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACga                    >  1:873608/1‑141 (MQ=255)
                                                                                                                    aaaaTTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATAAGATACTTAACATTAAAGTTGCAATTAAGAGGATTTTTATACAACATGGAGAAAAAGAAGACGAATGAAAATGTTTGGAACgg                    >  2:614053/1‑140 (MQ=255)
                                                                                                                         tGTAGATAAGCGTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGt               <  2:741802/141‑1 (MQ=255)
                                                                                                                            aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAGGTTTTGAAGCAGGAGTaac            >  2:708888/1‑139 (MQ=255)
                                                                                                                            aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACa            >  1:90812/1‑141 (MQ=255)
                                                                                                                                  gCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtc      <  1:686783/141‑1 (MQ=255)
                                                                                                                                  gCTCGGCGTCGGTGGCATGAGCACCGTTTATCATGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtc      <  2:474661/141‑1 (MQ=255)
                                                                                                                                   cTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGATGTGAACGAGAAGTACATAAcaca     >  2:156466/1‑141 (MQ=255)
                                                                                                                                   cTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAGACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtca     >  2:634909/1‑141 (MQ=255)
                                                                                                                                    ttGGCGTCGGTAGCATGAGTACCGTTTAAATAGCAGAAGATCCGATACTAAACATAAAAGTTGCAATTAAGGCGAGTTTAATACCACCTATAGAAAAAGAAGAAACATTAAAACGATTAGAACGAGAAGTACATaaaccat    >  2:196446/1‑136 (MQ=255)
                                                                                                                                     tGGCGTCGGTGGTATTAGTATCGTTTATCTATCTGAAGATACGATACTTAACATTAAAGTTTTACTTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAATACGTTTTGAACGAGAAGTACATAACtcatc   <  1:354140/141‑1 (MQ=255)
                                                                                                                                      ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca  >  1:442247/1‑141 (MQ=255)
                                                                                                                                      ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca  >  2:727853/1‑141 (MQ=255)
                                                                                                                                      ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcatca  <  2:355042/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
GGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA  >  CP000730/1232010‑1232284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: