Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT99.1% 363.2 / NA 114C146Y (TGT→TAT) ygaDABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (66/46);  minor base G (0/1);  total (66/48)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTG  >  CP000730/2010226‑2010497
                                                                                                                                         |                                                                                                                                      
tttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTCAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATGGTTATATAAt                                                                                                                                     >  1:662128/1‑141 (MQ=255)
tttCACGTGTCAATTCGCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                     >  1:496586/1‑141 (MQ=255)
       tgtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCa                                                                                                                              >  2:817314/1‑141 (MQ=255)
       tgtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCa                                                                                                                              >  2:146757/1‑141 (MQ=255)
           aaTTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGTGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGTACATTATGGATAGTGCAATAACAATTGTTATATAATCGAACCAAata                                                                                                                          <  2:638000/141‑1 (MQ=255)
               ttCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATAttaa                                                                                                                      >  2:725155/1‑139 (MQ=255)
                tCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAt                                                                                                                     <  2:520943/141‑1 (MQ=255)
                  ttAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTa                                                                                                                   <  2:207886/141‑1 (MQ=255)
                               gcaatagTACAGCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATCATTGTTATATAAGCTAACCAAATATTCATTAACCCGGTTAAAAt                                                                                                      >  2:697165/7‑141 (MQ=255)
                               gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAAt                                                                                                      >  2:615496/1‑141 (MQ=255)
                               gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAAAAGTGGTGATAAAGTCAATTTCAAATATAAAAAGAAAATTATGGATAGTGCAATAATAGTTGTTATATAATCTAACAAAATATTCATTAAACCGGGTaaaaa                                                                                                      >  2:256409/1‑140 (MQ=255)
                               gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAAAAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAAt                                                                                                      <  1:929385/141‑1 (MQ=255)
                               gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAAAAGTGCTGCTAAAGTCAATTTCACATATAAAAAGAACCTTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTATCACGGTTaaaaa                                                                                                      >  2:558349/1‑140 (MQ=255)
                                 aaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGa                                                                                                    >  2:326359/1‑141 (MQ=255)
                                 aaaCGGACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGa                                                                                                    <  2:718949/141‑1 (MQ=255)
                                      tACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCt                                                                                               >  2:93982/1‑141 (MQ=255)
                                          ccGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttg                                                                                           <  2:802883/141‑1 (MQ=255)
                                            tttAAAACGGAAAAAGGGAAGATAAACCGGTCTGCTAAAGTCAATTCCACATCCAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATAGAATCTTACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgtt                                                                                         <  2:469925/140‑1 (MQ=255)
                                            gTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATGTAAAGAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgtt                                                                                         <  1:676929/141‑1 (MQ=255)
                                             ttAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAATATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgttt                                                                                        <  2:762148/141‑1 (MQ=255)
                                             ttAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgttt                                                                                        <  2:762047/141‑1 (MQ=255)
                                             ttAAAATGTAAAATGGGAAGATAAACAGGGCTGCCAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgttt                                                                                        <  2:820671/141‑1 (MQ=255)
                                             ttAAAATGTAAAAAGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTACATAATACCATCCATTATTCATTAACCCGGTTAAAATGAAATCTtttgttt                                                                                        <  2:653111/141‑1 (MQ=255)
                                              taaaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgtttg                                                                                       >  1:534641/1‑141 (MQ=255)
                                               aaaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTttgtttgt                                                                                      >  2:559220/1‑141 (MQ=255)
                                               aaaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTttgtttgt                                                                                      <  1:430138/141‑1 (MQ=255)
                                                aaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATCATTGTTATATAATCTACCCAAATATTCATTAACCCGGTTCAAATGAAATCTTTtttttgtt                                                                                     <  2:581236/141‑1 (MQ=255)
                                                aaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTtgtttgtt                                                                                     <  2:504928/141‑1 (MQ=255)
                                                   tgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCaa                                                                                  <  2:444540/141‑1 (MQ=255)
                                                    gtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAc                                                                                 <  2:11929/141‑1 (MQ=255)
                                                    gtaaaagaGGAAGATAAACTGTGCTTCTAAAGTAAATATCACATCAAAAAAGAACATGATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAc                                                                                 <  1:767079/141‑1 (MQ=255)
                                                         atgGGAAGATTAACAGTGCTGCTAAACTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATACTCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            <  1:610954/141‑1 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            <  1:847961/141‑1 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            >  2:320856/1‑141 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            >  2:824414/1‑141 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            <  1:81650/141‑1 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            <  1:597880/141‑1 (MQ=255)
                                                         atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            <  2:493958/141‑1 (MQ=255)
                                                           gggAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTa                                                                          >  2:795169/1‑141 (MQ=255)
                                                             gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                        >  1:948274/1‑141 (MQ=255)
                                                             gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGGTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                        >  1:324389/1‑141 (MQ=255)
                                                             gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACAAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                        >  2:76777/1‑141 (MQ=255)
                                                             gAAGATAAAAAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAAAtaattaat                                                                        >  2:860333/1‑141 (MQ=255)
                                                              aaGATAAAAAGGGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATc                                                                       <  2:478103/141‑1 (MQ=255)
                                                                   aaaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTAAACATCATTAATCActct                                                                  >  2:81744/1‑141 (MQ=255)
                                                                    aaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCActcca                                                                 >  2:79576/1‑139 (MQ=255)
                                                                    aaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACACGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTa                                                                 >  2:349592/1‑141 (MQ=255)
                                                                       aGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAGTCTAGat                                                              >  1:55463/1‑141 (MQ=255)
                                                                       aGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGat                                                              >  1:158255/1‑141 (MQ=255)
                                                                       aGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGat                                                              <  2:549913/141‑1 (MQ=255)
                                                                            tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAAATATTAc                                                         >  1:882681/1‑141 (MQ=255)
                                                                            tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATAATTAATCACTCTAGATAttcc                                                         >  1:997992/1‑139 (MQ=255)
                                                                             gctAAAGTCAATTTCACATCTAAAAACAACATTATGGATAGTACAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACt                                                        <  1:127444/141‑1 (MQ=255)
                                                                              ctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACtt                                                       >  2:901379/1‑141 (MQ=255)
                                                                               tAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTg                                                      >  1:263180/1‑141 (MQ=255)
                                                                                aaaGTCAATTTCACATCTAAAAAGAACATTATGTATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGa                                                     <  2:402501/141‑1 (MQ=255)
                                                                                 aaGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTTTAGATATTACTTGAc                                                    >  1:821890/1‑141 (MQ=255)
                                                                                  aGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGAcc                                                   <  1:512867/141‑1 (MQ=255)
                                                                                     cAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATCTAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTTCCCTat                                                >  2:931827/1‑140 (MQ=255)
                                                                                     cAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTAc                                                <  1:125067/141‑1 (MQ=255)
                                                                                     cAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTAc                                                >  1:215221/1‑141 (MQ=255)
                                                                                     cAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATAACTCTAGATATTACTTGACCTAc                                                >  2:101724/1‑141 (MQ=255)
                                                                                      aaTTTCACATCTAAAAAGATCATTATGTATAGTGAAACAATGTTGGTCACTTGATCTGATCAAATATTCATTAACATGGTTAAAATTAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACt                                               <  2:303450/141‑1 (MQ=255)
                                                                                        tttCACATTTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATACTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTg                                             <  2:719656/141‑1 (MQ=255)
                                                                                        tttCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTg                                             <  2:362974/141‑1 (MQ=255)
                                                                                            acaTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACACGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGatta                                         >  2:916192/1‑141 (MQ=255)
                                                                                              ctCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                       <  1:345374/140‑1 (MQ=255)
                                                                                              aTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                       <  1:590456/141‑1 (MQ=255)
                                                                                              aTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                       <  1:583630/141‑1 (MQ=255)
                                                                                              aTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                       <  1:590405/141‑1 (MQ=255)
                                                                                              aTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                       >  1:980597/1‑141 (MQ=255)
                                                                                               tCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGAttatta                                      >  2:930971/1‑141 (MQ=255)
                                                                                                cTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATTAAATCTTTTGTTTTTTAAACATAATTAATCACTCTAGATATTACTTTACCTACTTGCTTATTAg                                     >  2:327077/1‑141 (MQ=255)
                                                                                                cTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTATTTGATTATTAg                                     >  2:451531/1‑141 (MQ=255)
                                                                                                    aaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATa                                 >  2:588920/1‑141 (MQ=255)
                                                                                                    aaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATa                                 <  2:690991/141‑1 (MQ=255)
                                                                                                         aCATTATGGGTAGTGCAATAATAATTGTTATATAATCTAACCAAATATACATTAACCCAGTTAAAAAGAAATCTTTTGTGTGTTCACAATCATTAATCACTCTAGATATTACTTGACCTACTGGATTATTACCATCAAAtc                            >  2:243962/1‑141 (MQ=255)
                                                                                                         aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATGATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                            >  2:680867/1‑141 (MQ=255)
                                                                                                         aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCCTAAAAtt                            >  1:394835/1‑140 (MQ=255)
                                                                                                         aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                            >  1:498410/1‑141 (MQ=255)
                                                                                                         aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                            >  1:817922/1‑141 (MQ=255)
                                                                                                             tATGTATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGc                        <  2:884403/141‑1 (MQ=255)
                                                                                                             tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGc                        <  1:146757/141‑1 (MQ=255)
                                                                                                             tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGc                        <  1:817314/141‑1 (MQ=255)
                                                                                                                        cAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt             >  1:838815/1‑141 (MQ=255)
                                                                                                                        cAATAACAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt             >  2:737414/1‑141 (MQ=255)
                                                                                                                         aatgataatTGTTATATGATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATGACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg            <  2:666011/141‑1 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg            <  1:571414/141‑1 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg            <  1:661571/141‑1 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg            >  1:441793/1‑141 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACAAGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg            <  1:767755/141‑1 (MQ=255)
                                                                                                                               aatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATg      <  1:326359/141‑1 (MQ=255)
                                                                                                                               aatTGTTATATAATATAACCAAATATTCATTAACCCGGTTAAAAAGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATg      >  1:542865/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGACATCTTTTGTTTGTTCAACATCATTAATCACTCTGGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAATTGGt    >  1:287317/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAGGCTTGTAAATGGt    >  1:143061/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAATTGGt    >  1:530503/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt    >  1:777917/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt    >  1:684559/1‑141 (MQ=255)
                                                                                                                                 ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt    >  1:395294/1‑141 (MQ=255)
                                                                                                                                   gTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCGCTTAAAGCGTGTAAATGGTTg  <  1:515146/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTG  >  CP000730/2010226‑2010497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: