BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	597,770	A→T	V168V (GTA→GTT)	tufA →	elongation factor EF1A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	597,770	0	A	T	99.1%	303.6 / NA	105	V168V (GTA→GTT)	tufA	elongation factor EF1A
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (57/47); total (58/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ATCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATG  >  CP000730/597632‑597905

aTCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGGTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGGGCCGATGTTcc                                                                                                                                       >  2:791534/1‑141 (MQ=255)

 tctggTAATGTTGGTGTAAGAGGATGAGTAGTATTTTTAAACAAAGTTGACACGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGACGTTCGTGACTTATTAAGCGAATATGACTTCACAGGTGACGATGTTCCt                                                                                                                                      <  2:413748/137‑1 (MQ=255)

 tCACGTAACGTTTGTTTACCAGAATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTCGAAGTAGTAGCAATGGCAGTTCGTGACTTATTAAGCGAATATGCCTCCCCAGGTGACGATGTTCCt                                                                                                                                      <  2:268618/141‑1 (MQ=255)

 tCACGTAACGTTGGTGTACCAGGATTAGTAGTCTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAATTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      <  1:665016/141‑1 (MQ=255)

 tCACGTAACGTTGGTGTACCAGCATTAGTAGTTTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGATCTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      <  1:664915/141‑1 (MQ=255)

 tCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      >  1:760445/1‑141 (MQ=255)

 tCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      >  2:906407/1‑141 (MQ=255)

 tCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      >  1:526149/1‑141 (MQ=255)

 tCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      >  2:759373/1‑141 (MQ=255)

 acACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                      <  2:869678/140‑1 (MQ=255)

  cccggaaCGTTGGTTGACCAGCATTATGAGTATTCTTACCCACGGTTGACATGGGTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTg                                                                                                                                     <  2:846689/136‑1 (MQ=255)

  cccGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTg                                                                                                                                     <  2:766875/139‑1 (MQ=255)

  cACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGATGATGTTCCTg                                                                                                                                     <  2:672810/141‑1 (MQ=255)

  cACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTg                                                                                                                                     <  2:707822/141‑1 (MQ=255)

  cACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTg                                                                                                                                     <  2:187384/141‑1 (MQ=255)

   aCGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACAATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGt                                                                                                                                    >  1:233367/1‑141 (MQ=255)

   aCGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGAAATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTAACGATGTTCCTGt                                                                                                                                    >  1:883984/1‑141 (MQ=255)

    cGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTa                                                                                                                                   >  1:97504/1‑141 (MQ=255)

     gTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTaa                                                                                                                                  >  1:441196/1‑141 (MQ=255)

     gTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTaa                                                                                                                                  >  1:811926/1‑141 (MQ=255)

        aCGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCg                                                                                                                               >  2:356852/1‑141 (MQ=255)

           ttgttgTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGAGGAAGAATTATTGGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTCCCCAGGTGACGATGTTCCTTTAATCGCTg                                                                                                                            <  2:922533/141‑1 (MQ=255)

           ttGGTGTAACAGCATTGGTAGTATTATTAAACAAAGTTGGCATGGTTGGCGATGAAGAATTATTAGAATTAGTAGACATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTg                                                                                                                            <  2:394558/141‑1 (MQ=255)

            tGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCggg                                                                                                                           >  1:125301/1‑141 (MQ=255)

            tGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTgg                                                                                                                           >  1:933783/1‑141 (MQ=255)

            tGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTgg                                                                                                                           >  2:831673/1‑141 (MQ=255)

            tGGTGTACACGCATTAGTAGTATTCTTAAACAAAGTTGACAGGGAGGAAGCCCGCTAAGTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTgg                                                                                                                           >  1:234615/1‑141 (MQ=255)

            tGGGGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTgg                                                                                                                           <  2:195494/141‑1 (MQ=255)

               tgtACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGCCTTCCCAGGTGACGAGGTTCCGGTAATCGCTGGTTc                                                                                                                        >  2:447105/1‑141 (MQ=255)

                   ccAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCa                                                                                                                    >  1:824308/1‑141 (MQ=255)

                   ccAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCa                                                                                                                    >  1:40545/1‑141 (MQ=255)

                     tgCAGTAGTAATATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCAtt                                                                                                                  <  2:678208/140‑1 (MQ=255)

                     aGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGAAGAAAAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCAtt                                                                                                                  <  1:23860/141‑1 (MQ=255)

                      gCATTAGTAGTATTCTTAAACAAAGTTGACATGGTAGACGATGCAGAATTATTAGAATTAGAAGAAATGGAAGTTCGTGATTTATTAAGCGAATATCACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTa                                                                                                                 <  2:444401/141‑1 (MQ=255)

                         ttAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTaaaa                                                                                                              >  1:169263/1‑141 (MQ=255)

                            gtagtaTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTGCAGCATTAAAAGCt                                                                                                           >  1:391445/1‑141 (MQ=255)

                                     ttAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCGAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGc                                                                                                  >  2:892578/1‑141 (MQ=255)

                                     ttAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAggg                                                                                                  >  2:741491/1‑140 (MQ=255)

                                      tacaCAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTCCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCg                                                                                                 <  1:153391/138‑1 (MQ=255)

                                      tAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCg                                                                                                 >  1:588877/1‑141 (MQ=255)

                                      tAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCg                                                                                                 <  1:235665/141‑1 (MQ=255)

                                         aCAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATg                                                                                              >  1:985537/1‑141 (MQ=255)

                                              gTTGCCATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCaa                                                                                         <  1:540761/141‑1 (MQ=255)

                                              gTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCaa                                                                                         >  1:333938/1‑141 (MQ=255)

                                              gTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGGGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCaa                                                                                         >  1:381612/1‑141 (MQ=255)

                                              gTTGACAGGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCaa                                                                                         <  1:656104/141‑1 (MQ=255)

                                                 gACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGACGGCGATGCTCAATAc                                                                                      >  2:685383/1‑141 (MQ=255)

                                                   cATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACga                                                                                    >  2:309555/1‑141 (MQ=255)

                                                    aTGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTACCAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                   <  1:833624/141‑1 (MQ=255)

                                                    aTGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                   <  1:389161/141‑1 (MQ=255)

                                                    aTGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                   >  1:997237/1‑141 (MQ=255)

                                                     tGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaag                                                                                  <  2:40545/141‑1 (MQ=255)

                                                      ggTTGACGATGAAGAATTATTAGAATTAGTACAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaaga                                                                                 <  2:892474/141‑1 (MQ=255)

                                                       tttGACGATTAAGAATTATTAGAATGAGTAGAAATGGAAGTTCGTGTCTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaagaa                                                                                <  2:445340/140‑1 (MQ=255)

                                                       gTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATAGGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaagaa                                                                                <  1:461412/141‑1 (MQ=255)

                                                             gATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATc                                                                          <  1:356852/141‑1 (MQ=255)

                                                               tGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCtt                                                                        <  2:233367/141‑1 (MQ=255)

                                                                 aagaaTTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGGAGGCGATGCTCAATACGAAGAAAAAATCTTAg                                                                      <  1:712910/141‑1 (MQ=255)

                                                                       tattaGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTaa                                                                <  2:521800/141‑1 (MQ=255)

                                                                          taGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATgg                                                             >  1:663126/1‑141 (MQ=255)

                                                                          taGAATTAGTAGAAAAGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAAAACAAAAAAAAAATCTTAGAATTAATgg                                                             <  2:572596/141‑1 (MQ=255)

                                                                            gAATTAGTAGAAATGGAAGCTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGCCGGTCCTCAATACGAAGAAAAAATCCTAGAATTAAAGGaa                                                           >  2:323466/1‑141 (MQ=255)

                                                                              tttAGTAGGAATAGAAGTTCGTGCCTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGTATTAATGGAAGc                                                         <  1:303937/140‑1 (MQ=255)

                                                                              atgagtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGc                                                         <  1:538015/138‑1 (MQ=255)

                                                                                  gtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGTGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTa                                                     >  1:95252/1‑141 (MQ=255)

                                                                                      aaaTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGGAAAAAACTTAGAATTAATGGAAGCTGTAGAtc                                                 >  2:660615/1‑140 (MQ=255)

                                                                                      aaaTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGTTGTAGATa                                                 >  2:523219/1‑141 (MQ=255)

                                                                                      aaaTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAACCTGTAGaga                                                 >  2:463950/1‑139 (MQ=255)

                                                                                      aaaTGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGAAGTTCCTGTAATCGCCGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATa                                                 >  1:715833/1‑141 (MQ=255)

                                                                                      aaaTGGAAGTTCGTGACTTATTAAGCGAATATGACTTACCAGGTGACGATGAACCTTTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGCTGCTCAATACGAGGAAAAAATCTTAGAATTAATGGAAGCTGTAGATa                                                 >  2:856218/1‑141 (MQ=255)

                                                                                           gAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTAc                                            >  2:749649/1‑141 (MQ=255)

                                                                                            aaGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCGGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATCCGAAGAAAAACTCTTAGAATTAATGGAAGCTGTGGATACTTAct                                           >  1:287954/1‑140 (MQ=255)

                                                                                            aaGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGAGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTCTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCAGTAGATACTTACa                                           >  2:824398/1‑141 (MQ=255)

                                                                                             aGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACAt                                          <  1:999201/141‑1 (MQ=255)

                                                                                              gTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTGAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATATTAGAATTAAGGGAAGCTGTAGATACTTACCtt                                         >  2:204687/1‑141 (MQ=255)

                                                                                              gTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGGTACTTACAtt                                         >  1:408330/1‑141 (MQ=255)

                                                                                                   tGACTTATTAAGGGAATATGACTTGCAAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTAAAAAAAAAAAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAAc                                    <  2:97504/141‑1 (MQ=255)

                                                                                                        tattaAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAg                               <  2:304622/141‑1 (MQ=255)

                                                                                                         attaAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGa                              >  2:320004/1‑141 (MQ=255)

                                                                                                            aaGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACg                           <  1:627240/141‑1 (MQ=255)

                                                                                                                gAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGAt                       >  2:749809/1‑141 (MQ=255)

                                                                                                                gAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGAt                       >  2:872861/1‑141 (MQ=255)

                                                                                                                      gACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGAc                 <  1:759373/141‑1 (MQ=255)

                                                                                                                      gACTTCCCAGGTGACGATGGTCCTGTAAGCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGAc                 <  1:14639/141‑1 (MQ=255)

                                                                                                                       aCTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAGAAGCTTTAGTAGGTGATGCTCAATACGAAGACAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAATTCCAGGACGTGATTTTGACa                >  2:162135/1‑141 (MQ=255)

                                                                                                                       aCTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACa                >  2:162818/1‑141 (MQ=255)

                                                                                                                       aCTTCCCAGGTGACGATGTTCCTGTAATCGCTGATTCAGCATTAAAAGCGTTAGAAGGCGATGCTCAATACGAAGAACAAATCTTAGAATTAATGGAAGCTGTAGATACTTAAATCCAAACTCCGGAACGTGATTCTAACa                >  2:519527/1‑141 (MQ=255)

                                                                                                                        cTTCCCAGGCAACGATCTTCCTGTAATCGCTGTTGCAGCATGAAAAGCGTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTGAGAATGGAAGGAAGCCGTGAATAGTTACATTCCAACTGCAGAACGTGATTCGGaaca               >  2:308026/1‑138 (MQ=255)

                                                                                                                             catgggACGATGTTCCTGTAAGCGCGGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCAt          <  2:433073/136‑1 (MQ=255)

                                                                                                                             cAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCAt          <  2:990629/141‑1 (MQ=255)

                                                                                                                             cAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCAt          <  2:444453/141‑1 (MQ=255)

                                                                                                                              aGGTGACGATGTTCCTGTAATCGCAGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGCAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCAtt         >  2:204709/1‑141 (MQ=255)

                                                                                                                               ggTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACCAACCATTc        >  2:817240/1‑141 (MQ=255)

                                                                                                                               ggTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTc        >  1:537274/1‑141 (MQ=255)

                                                                                                                                gtaaCGATGTTCCGGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCa       <  2:526149/138‑1 (MQ=255)

                                                                                                                                gTGACGATGTTCCTTTAATCGCTGGTTGAGCATTAAAAGCTTTAGAAGGCGATGCTCAAAAAGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCa       <  2:760445/141‑1 (MQ=255)

                                                                                                                                gTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCa       <  1:326827/141‑1 (MQ=255)

                                                                                                                                  gACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATTCGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCatg     >  1:596821/1‑141 (MQ=255)

                                                                                                                                    cGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCatgat   >  2:313942/1‑141 (MQ=255)

                                                                                                                                     gATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTGACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCatgatg  >  1:567153/1‑141 (MQ=255)

ATCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATG  >  CP000730/597632‑597905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`