Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 973,213 C→A E175* (GAG→TAG)  yitU ← possible HAD superfamily hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730973,2130CA98.6% 211.1 / NA 70E175* (GAG→TAG) yitUpossible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (42/27);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCAT  >  CP000730/973075‑973351
                                                                                                                                          |                                                                                                                                          
tATCTTCATCACCGAGTGCCATAATATTTTTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCACTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCCAGGCTaaa                                                                                                                                          >  1:770953/1‑141 (MQ=255)
       atcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATc                                                                                                                                   >  2:995756/1‑141 (MQ=255)
          aCCGAATGCAATAATTTTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGc                                                                                                                                >  1:740967/1‑141 (MQ=255)
          aCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTCACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATATAAGTGACAGGGGAAGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGc                                                                                                                                >  1:331979/1‑141 (MQ=255)
           ccGAATGGAAGAAGATTATTTCGGTCAATAGTTAAAAATTGTCTAACTTGATCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAAGTTCAATGACAGGGAATGGTGCGCACCAGCGTCGATGCTAAATATGATCGGCa                                                                                                                               <  2:149055/141‑1 (MQ=255)
            cGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCAt                                                                                                                              >  1:75196/1‑141 (MQ=255)
              aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                            >  1:522494/1‑141 (MQ=255)
              aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                            >  2:482062/1‑141 (MQ=255)
              aaTGCAATAATATAATTTCGGTCAATATTTAAAAATTGTCTAACTTGATCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                            >  2:957928/1‑141 (MQ=255)
                tGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaa                                                                                                                          >  2:650980/1‑141 (MQ=255)
                tGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaa                                                                                                                          >  2:301299/1‑141 (MQ=255)
                 gCAATAATATTATTTCGGTCAATATTTAAAACTTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCCATGCCAAATCTCCTCGGCATcaaaa                                                                                                                         >  2:643657/1‑141 (MQ=255)
                         attattTCGGTCAGTATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAg                                                                                                                 <  2:716391/141‑1 (MQ=255)
                         attattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAg                                                                                                                 <  2:872908/141‑1 (MQ=255)
                             tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCatt                                                                                                             >  2:65171/1‑140 (MQ=255)
                             tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCata                                                                                                             >  2:417969/1‑141 (MQ=255)
                               tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAtt                                                                                                           <  1:786483/141‑1 (MQ=255)
                                cGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttt                                                                                                          >  2:981524/1‑141 (MQ=255)
                                cGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttt                                                                                                          <  1:995756/141‑1 (MQ=255)
                                      atatTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAtt                                                                                                    <  1:306326/141‑1 (MQ=255)
                                        atTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTc                                                                                                  >  1:83700/1‑141 (MQ=255)
                                         tttAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCa                                                                                                 >  1:880132/1‑141 (MQ=255)
                                         tttAAAAATTGTCTAACTTGCTCAATGCCTCTTGATTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGAATATTTTTGATTTCa                                                                                                 >  2:167255/1‑141 (MQ=255)
                                                       taCTTGCTCAATGCCTCTTGCTTTTTTAATACCAAGTTTGACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttc                                                                                   <  2:447604/140‑1 (MQ=255)
                                                       agcTTGCTCAGTGCCTCTGGCTCTATTAAGACCGAGTTTTACACTTCCAATGACAGGCAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTGGATTTCAGGTATTTTACtctc                                                                                   <  2:951583/139‑1 (MQ=255)
                                                       aaCTTGCTCAATGCCTTTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttc                                                                                   <  2:820493/141‑1 (MQ=255)
                                                       aaCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttc                                                                                   <  2:411149/141‑1 (MQ=255)
                                                       aaCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttc                                                                                   <  2:958449/141‑1 (MQ=255)
                                                         cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGCCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttctt                                                                                 >  1:143638/1‑141 (MQ=255)
                                                         cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTCTTTTAGTttctt                                                                                 >  1:683803/1‑141 (MQ=255)
                                                         cTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttctt                                                                                 >  2:918513/1‑141 (MQ=255)
                                                           tGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGGATGGTGAGCCCCTGCGTCGATGCTATATTGTATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCg                                                                               <  2:600143/141‑1 (MQ=255)
                                                            gCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                              <  1:273485/141‑1 (MQ=255)
                                                            gCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                              <  1:793941/141‑1 (MQ=255)
                                                            gCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCgg                                                                              <  1:220063/141‑1 (MQ=255)
                                                             cTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                             >  2:424342/1‑141 (MQ=255)
                                                             cTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                             >  2:759348/1‑141 (MQ=255)
                                                                       ctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaca                                                                   >  2:64886/1‑139 (MQ=255)
                                                                               attaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTATTCGGCTTCAATTTACATTGAGGt                                                           >  1:861991/1‑141 (MQ=255)
                                                                                     aCCATGTTTTACAATTTCGAGGACAGTGAATGGTGCGCCCCAGCGTCGATGCTAAATAAGATCAACATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     <  2:203382/141‑1 (MQ=255)
                                                                                     aCCAAGTTTTACAATTTTAATGACAGGTAATGGGGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     <  1:147339/141‑1 (MQ=255)
                                                                                     aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     <  2:861057/141‑1 (MQ=255)
                                                                                     aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     <  1:399746/141‑1 (MQ=255)
                                                                                     aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     <  1:930044/141‑1 (MQ=255)
                                                                                     aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                     >  1:421436/1‑141 (MQ=255)
                                                                                          gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGCCTTCATTTCTTTTATTATCTGCTGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                                <  1:983645/141‑1 (MQ=255)
                                                                                          gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                                <  2:52367/141‑1 (MQ=255)
                                                                                          gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                                <  1:886392/141‑1 (MQ=255)
                                                                                           ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                               >  1:141432/1‑141 (MQ=255)
                                                                                              tACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                            >  1:181756/1‑141 (MQ=255)
                                                                                              tACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                            >  1:981248/1‑141 (MQ=255)
                                                                                               aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                           >  2:271919/1‑141 (MQ=255)
                                                                                               aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                           >  1:18239/1‑141 (MQ=255)
                                                                                               aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAAGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                           >  2:546318/1‑141 (MQ=255)
                                                                                               aCAATCTCAATGACAGGGAATGGTGGGCCCCAGCGTCGATGCTAAATATGCTAGGCATGAAAATCAGTAAGCATATTTTTGATTGCAGGTATTTTACTGTATTCGGCTTCAATTAAAATTGAGGCAGGGGATTCTTTTAAg                                           >  2:291478/1‑141 (MQ=255)
                                                                                                cAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGGTTTTTTCAAGt                                          >  1:478357/1‑141 (MQ=255)
                                                                                                       aaTGGCAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAg                                   >  1:675919/1‑141 (MQ=255)
                                                                                                       aaTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAg                                   >  2:31402/1‑141 (MQ=255)
                                                                                                               ggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATGGAGGTAGGGGATTCTTTCAAGTGGAGAAGTAAATTAc                           >  1:410620/1‑141 (MQ=255)
                                                                                                                        cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa                  <  1:759348/141‑1 (MQ=255)
                                                                                                                        cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa                  <  1:424342/141‑1 (MQ=255)
                                                                                                                        cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa                  >  1:444882/1‑141 (MQ=255)
                                                                                                                        cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa                  >  2:130395/1‑141 (MQ=255)
                                                                                                                        cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa                  >  2:863620/1‑141 (MQ=255)
                                                                                                                            ccAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCt              <  2:740967/141‑1 (MQ=255)
                                                                                                                                  tCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGCGTCTTTCAAGTGGACAAGGAAGTTACCAGTTTCAATTCTTGGAtt        >  2:243568/1‑141 (MQ=255)
                                                                                                                                  tCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAtt        >  1:245255/1‑141 (MQ=255)
                                                                                                                                      tGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAccc    <  1:981524/141‑1 (MQ=255)
                                                                                                                                      tGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGCCTTGGAGTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTAccc    >  1:771856/1‑141 (MQ=255)
                                                                                                                                      tGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTCTCGATTACAGGTATTTTCCTGTCTTCGGCTTCAATTACAATACAGGGCGGGGAGTCTTTAACGTGGACAACTACAGTACCAAAGTGAAATCTTGGAATAcac    >  2:161233/1‑139 (MQ=255)
                                                                                                                                        cTCACTCTGGTCGGCCTCAATATGAGTTAGCATATTTTTCATTTCCGGTATATAACTTTCCTCGTTTTTAATTATAATTGAGGGAGGGGATGCTTTCCAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCAt  <  2:850423/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCCAT  >  CP000730/973075‑973351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: