Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,002,200 T→G M173R (ATG→AGG)  mecA2 → competence negative regulator MecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,002,2000TG98.6% 251.1 / NA 73M173R (ATG→AGG) mecA2competence negative regulator MecA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (23/49);  total (23/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAT  >  CP000730/1002063‑1002333
                                                                                                                                          |                                                                                                                                     
atgatCTAAACGTCAAAAGTCTACAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCAGATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:228783/137‑1 (MQ=255)
aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:882388/141‑1 (MQ=255)
aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAAGCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  1:289295/141‑1 (MQ=255)
aGGTTCTAAACGGCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                                                    <  2:228689/141‑1 (MQ=255)
 ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:129641/141‑1 (MQ=255)
 ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   >  2:198385/1‑141 (MQ=255)
 ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:59606/141‑1 (MQ=255)
 ggTTCTAAACGTCAAAAGTATTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCAGATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                   <  1:660447/141‑1 (MQ=255)
   ttCTAAACGTAAAAAGTCTACAGCACGAAAAAATACAACAACACACATTATGAAATTTAACGATTTCGAACTGTCTATGAATTAAGCCAGGTAGATCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGa                                                                                                                                 <  1:234367/141‑1 (MQ=255)
     cTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATg                                                                                                                               <  2:604376/141‑1 (MQ=255)
       aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:15707/141‑1 (MQ=255)
       aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:15504/141‑1 (MQ=255)
       aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAAGTAATTCGTTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTggtggt                                                                                                                             <  1:677802/141‑1 (MQ=255)
       aaaCGTCAAAAGTCCTCAGCACGTAAAAATACAAGAACAACCACCGTGACATTTAACGATTTAGAAGAAGGGAATAATTACGCATAGCCCGGCATTCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                                                                             <  1:233992/141‑1 (MQ=255)
        aaCGTCAACAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            <  1:359107/141‑1 (MQ=255)
        aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  1:920508/1‑141 (MQ=255)
        aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  2:467079/1‑141 (MQ=255)
        aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                            >  2:418205/1‑141 (MQ=255)
        aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTCAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATCTGTTTTATAGGGTTGATGGTa                                                                                                                            <  1:511651/141‑1 (MQ=255)
              aaaaTTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAATTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                      <  1:350915/141‑1 (MQ=255)
                aaGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattat                                                                                                                    <  2:780228/141‑1 (MQ=255)
                 agtccgcAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatt                                                                                                                   <  1:212551/135‑1 (MQ=255)
                 aGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGTGTTGATGGTACttattatt                                                                                                                   >  1:16374/1‑141 (MQ=255)
                         gCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGta                                                                                                           >  1:99407/1‑141 (MQ=255)
                                  aaTACAAGAACAATCATTGTGCAATATAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATACAATAACTACAGAGTTTGAAGATTTGATACATAGGGTTGATGGTACTTATTATTAGGCTGTATATTTTGAt                                                                                                  >  2:185666/1‑141 (MQ=255)
                                              aTCATTTTGAAATTTAACGATTTAGAAGATGGTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGTAGATTTGTGTTATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  2:27629/141‑1 (MQ=255)
                                              aTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  1:454716/141‑1 (MQ=255)
                                              aTCAGTGTGAAATTTAATGATTTAGAAGATTTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                      <  1:792611/141‑1 (MQ=255)
                                                cATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                    >  2:269375/1‑141 (MQ=255)
                                                 aTTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaa                                                                                   <  1:157487/141‑1 (MQ=255)
                                                 aTTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaa                                                                                   <  1:649948/141‑1 (MQ=255)
                                                    gtgAAATTTAACGATTTAGAAGAGGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                                                                <  1:179450/141‑1 (MQ=255)
                                                        aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACTGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:654961/141‑1 (MQ=255)
                                                        aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:921868/141‑1 (MQ=255)
                                                        aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCa                                                                            <  2:769073/141‑1 (MQ=255)
                                                                         gaTTTTATTAATTATGCATATCATAGCAATCCAATAATTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGt                                                           <  2:636188/141‑1 (MQ=255)
                                                                         gaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGt                                                           <  1:549914/141‑1 (MQ=255)
                                                                          aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTc                                                          >  1:607216/1‑141 (MQ=255)
                                                                          aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTc                                                          >  2:467942/1‑141 (MQ=255)
                                                                          aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTAAAGtt                                                          >  2:720551/1‑140 (MQ=255)
                                                                           tGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGATTGATGGTACTAATTATTATGCTGTATATTTTGATAATCATGTTGATCAAGAAGTCATTAATGATAGTTACattaa                                                         >  2:713465/1‑137 (MQ=255)
                                                                            gTTATTAATTATGCATATCATAGCAAGCCAATAACTACAGAGTTTGAAGAGTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCaa                                                        <  2:248083/141‑1 (MQ=255)
                                                                                attaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTg                                                     <  1:652323/141‑1 (MQ=255)
                                                                                  tgattaTGCATATCATATCAATCCAATAACTACAGAGTTTGAACATTTGTTATATAGGGTTGATGGTACTTATTATTATGCCGTATATTTTGATAGTCATGTTGATCAAGAAGCCATTCATGATAGTTACAGTCAATTGCt                                                   <  2:385387/139‑1 (MQ=255)
                                                                                  taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  2:646068/141‑1 (MQ=255)
                                                                                  taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:817222/141‑1 (MQ=255)
                                                                                  taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  2:87267/141‑1 (MQ=255)
                                                                                  taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:423243/141‑1 (MQ=255)
                                                                                  taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                   <  1:432479/141‑1 (MQ=255)
                                                                                     ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGa                                                >  2:557572/1‑141 (MQ=255)
                                                                                     ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGa                                                >  1:395425/1‑141 (MQ=255)
                                                                                        tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAAtt                                             >  2:46171/1‑141 (MQ=255)
                                                                                        tGCATATCACAGCAATCCAATCACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAAtt                                             <  1:111190/141‑1 (MQ=255)
                                                                                              tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTa                                       >  2:820690/1‑141 (MQ=255)
                                                                                              tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTa                                       >  2:820806/1‑141 (MQ=255)
                                                                                              tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATATTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTGAGGGTAGTTACAGTCATTTGCTTGATTTTGGttt                                       >  1:33247/1‑140 (MQ=255)
                                                                                                aTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATAAAGAAGTCATTAATGATAGTTACAGTCACTGGCTGGAATTGACTTATc                                     >  2:918472/1‑141 (MQ=255)
                                                                                                  aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCa                                   >  1:830631/1‑141 (MQ=255)
                                                                                                    cAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAac                                 >  2:847504/1‑141 (MQ=255)
                                                                                                       tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacaga                              >  2:302371/1‑141 (MQ=255)
                                                                                                         cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacagaca                            >  2:173519/1‑141 (MQ=255)
                                                                                                           ttgagtACAGGGTTTGAAGTTTTGGTATATAGGGGTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAGGTCATGAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:385793/136‑1 (MQ=255)
                                                                                                           aTAATTATAGCGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATTCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:346703/141‑1 (MQ=255)
                                                                                                           aTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:883149/141‑1 (MQ=255)
                                                                                                           aTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGATGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                          <  2:850414/141‑1 (MQ=255)
                                                                                                               cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:21696/141‑1 (MQ=255)
                                                                                                               cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:50806/141‑1 (MQ=255)
                                                                                                               atagagagTTTGAAGAGTTGGTATATATGGTTTATTGTACTTATTGTTACGCTGTATATTTTGATAGTCATTTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAACTTGCTAATCCAACTGacagaaaag                      <  2:582000/137‑1 (MQ=255)
                                                                                                               atACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacag                      <  1:244511/140‑1 (MQ=255)
                                                                                                                      gTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGttta               <  2:266917/141‑1 (MQ=255)
                                                                                                                            aGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTaaa         <  2:415646/141‑1 (MQ=255)
                                                                                                                             gATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAAt        <  2:594483/141‑1 (MQ=255)
                                                                                                                                   ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAt  <  1:46171/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTAT  >  CP000730/1002063‑1002333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: