BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,455,698	2 bp→AT	coding (1216‑1217/1260 nt)	USA300HOU_2315 →	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,455,698	0	C	A	85.0%	309.0 / 52.5	108	coding (1216/1260 nt)	USA300HOU_2315	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (34/57); minor base . (10/6); total (44/64)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP000730	2,455,699	0	A	T	97.3%	334.6 / ‑5.4	110	H406L (CAT→CTT)	USA300HOU_2315	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/3); new base T (44/63); total (44/66)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC  >  CP000730/2455569‑2455831

||

gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaaa                                                                                                                           <  2:133192/141‑1 (MQ=255)

 ttAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAAc                                                                                                                          <  1:313907/141‑1 (MQ=255)

 ttAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTAATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAAc                                                                                                                          >  2:492583/1‑141 (MQ=255)

 ttAAACAAGCACCAAAAAGTGACGATTTAACAAACAATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAAc                                                                                                                          <  2:536032/141‑1 (MQ=255)

      cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaag                                                                                                                     >  1:124769/1‑141 (MQ=255)

      cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaag                                                                                                                     <  1:853457/141‑1 (MQ=255)

      cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaaa                                                                                                                     >  1:250728/1‑140 (MQ=255)

        aGCACCACAAAGTGACGATTTAACAAACGATTCTAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAATGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaagaa                                                                                                                   <  2:553827/141‑1 (MQ=255)

        aGCACCACAAAGGGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaagaa                                                                                                                   <  2:332295/141‑1 (MQ=255)

         gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAAc                                                                                                                  >  1:434386/1‑141 (MQ=255)

         gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAAc                                                                                                                  >  1:114447/1‑141 (MQ=255)

         gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAGAACTGAAGAAc                                                                                                                  <  2:710107/141‑1 (MQ=255)

         gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc                                                                                                                  >  1:859533/1‑141 (MQ=255)

          cacgaaaaaggcaCGTTTTAACAAACTATTCAAATGTAGATTAAATGGGGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATGAGTTGTAATTGATTCAAAAAAA‑TTATCGAAAAAACTGTAGAACa                                                                                                                 <  2:548310/129‑1 (MQ=255)

             cacaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCTTCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAAtt                                                                                                              >  2:602643/1‑140 (MQ=255)

              acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                             <  1:749265/141‑1 (MQ=255)

                   gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGATCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                        <  2:138055/141‑1 (MQ=255)

                   gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                        <  2:770202/141‑1 (MQ=255)

                   gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                        <  2:589157/141‑1 (MQ=255)

                   gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAACTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                        <  2:415401/141‑1 (MQ=255)

                   gTGACGATTTAACAAACGAGTGAAATGAAGATGAAATATAGCAAGCATTAAAAGAACCTGCAACTTATAAAGAAAACATACGTTGATCAGTTGTAATTAATGCA‑AAAAAATTATCAAAAAAAGTGAAGAGCAATCTTAAGa                                                                                                        >  2:126824/1‑141 (MQ=255)

                   agcaCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                        <  2:117513/138‑1 (MQ=255)

                     gACGATTTAACAAACGATTCAAATGAAGATGGAAGAGCGCACTCATTAAAAGAACCTGCGTGTTATAAAGAAGACAGACGTTCTTCGGTTGTAAAAAAAAAA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATa                                                                                                      <  2:605556/141‑1 (MQ=255)

                     gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATa                                                                                                      <  2:627232/141‑1 (MQ=255)

                       cGATTTAAATAGAGATTCAAATGAAGATGAAGTAGAGTAATGATTAAAAGAACCGGCGACTGATAAAGAAGATCGACGTTCATCAGTTCTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                    <  2:34006/141‑1 (MQ=255)

                        gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                   >  1:156267/1‑141 (MQ=255)

                        gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                   <  1:494435/141‑1 (MQ=255)

                        gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCAACAGTCGTACATGATGCAGAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                   >  2:303362/1‑141 (MQ=255)

                         aTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATaaaaa                                                                                                  >  1:558883/1‑141 (MQ=255)

                         aTTTAACAAACGATTACAATGAATATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATaaaaa                                                                                                  >  2:962598/1‑141 (MQ=255)

                                      ttaaaaTAAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAATATCAAAAAAACTGAAGAACAATCTTCGGATAAAAACAAATAATAACaa                                                                                     <  2:946266/138‑1 (MQ=255)

                                      tccAAATTAATATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTGTAAAGAAGACTGACGTTCATCAGTTGTAAAAAAAAAA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                     <  2:945260/139‑1 (MQ=255)

                                         aaaTGAAGATGAAAGAGAGAAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc                                                                                  >  1:547838/1‑141 (MQ=255)

                                                aataaaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt                                                                           <  2:674624/137‑1 (MQ=255)

                                                 aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTa                                                                          <  1:142384/141‑1 (MQ=255)

                                                  tGAAATATAGCAAGCATGAAAAGAAGTTGGGACTTATAAAGACGTTAGACGTTCATTAGTTGTAATTTATGCA‑AAAAAAAAAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                         <  2:407697/141‑1 (MQ=255)

                                                  tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                         <  1:10610/141‑1 (MQ=255)

                                                  tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                         <  2:686648/141‑1 (MQ=255)

                                                  tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                         >  2:835498/1‑141 (MQ=255)

                                                  agAAATAGAGCAATCATTAAAAGACCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                         <  1:10687/140‑1 (MQ=255)

                                                    aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTa                                                                       <  1:624152/141‑1 (MQ=255)

                                                    aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTa                                                                       <  1:788867/141‑1 (MQ=255)

                                                            cAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa                                                               <  1:295841/141‑1 (MQ=255)

                                                            cAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa                                                               <  1:93821/141‑1 (MQ=255)

                                                              aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                             <  1:194653/141‑1 (MQ=255)

                                                                 aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGGTGCTTAAAAA‑TTCTGTAATGGACTGAAGAACAATCTTCAGATAAAAACAAATAATAACGAAGCGCTTGCTAGTACCTCTTAAAAAga                                                          >  1:476499/1‑141 (MQ=255)

                                                                 aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAga                                                          >  2:309986/1‑141 (MQ=255)

                                                                 aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAga                                                          >  2:923467/1‑141 (MQ=255)

                                                                 aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAga                                                          >  2:193817/1‑141 (MQ=255)

                                                                  ttAAAAGAACCTGCGACTTATAAAGACGACAGACGTTCATCCGTTGTACTTGCTGCA‑AAAAAATTATCGAAAAAAATGAAGACGAATCAGCAGATCACCACACCTAATAACAAAGCGCTTGCTAGTACCTCTTGAAAAgaa                                                         >  2:49887/1‑140 (MQ=255)

                                                                      aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATTGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                                     <  1:670893/141‑1 (MQ=255)

                                                                      aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                                     <  1:552871/141‑1 (MQ=255)

                                                                      aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                                     <  1:398490/141‑1 (MQ=255)

                                                                         aaCCTGCAACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCGTAAAAAGATGATGCTa                                                  <  2:359352/141‑1 (MQ=255)

                                                                          aCCTGCGATTTATAAAGAAGACGGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                 <  2:712238/141‑1 (MQ=255)

                                                                          aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                 >  1:548368/1‑141 (MQ=255)

                                                                          aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                 >  1:628553/1‑141 (MQ=255)

                                                                          aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                 <  2:816545/141‑1 (MQ=255)

                                                                          aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAg                                                 <  2:816563/141‑1 (MQ=255)

                                                                            ctgctacagaaacagaagaCAGACTGTCATCATTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCCTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                               <  1:207831/128‑1 (MQ=255)

                                                                            cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAACACTGAAGAACAATCTTCAGATAAAAACAAATAATAACGAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                               <  1:197015/141‑1 (MQ=255)

                                                                            cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                               <  1:30765/141‑1 (MQ=255)

                                                                            cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATCGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAGAAACAGATACTGTGCTGGTCGTCGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                               <  1:728402/141‑1 (MQ=255)

                                                                             tGCTACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                              <  1:93843/141‑1 (MQ=255)

                                                                             tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                              <  2:124769/141‑1 (MQ=255)

                                                                             tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                              <  1:401106/141‑1 (MQ=255)

                                                                             cgCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                              <  1:903424/140‑1 (MQ=255)

                                                                               cgatgtataAAGAAGACAGACGTTCATCAGGTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                            <  2:80032/136‑1 (MQ=255)

                                                                               cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                            <  2:564781/141‑1 (MQ=255)

                                                                                 aCTTATAAAGAAGACAGACGTTCATCTGTTGTAATTGATGCA‑AAAAAATTTTCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAgcgc                                          >  1:185304/1‑141 (MQ=255)

                                                                                 aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGAAGCTAGCAAgcgc                                          >  2:892416/1‑141 (MQ=255)

                                                                                 aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                          >  1:956273/1‑141 (MQ=255)

                                                                                   ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt                                        >  1:587704/1‑141 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      >  2:517871/1‑141 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      <  2:114447/141‑1 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      <  2:859533/141‑1 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      >  1:864177/1‑141 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      >  1:453405/1‑141 (MQ=255)

                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                      >  2:925274/1‑141 (MQ=255)

                                                                                              acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                             <  1:995389/141‑1 (MQ=255)

                                                                                                agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                           <  1:797363/141‑1 (MQ=255)

                                                                                                agacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCACTTTTCTATACtatat                           <  1:492583/141‑1 (MQ=255)

                                                                                                agacGTTCATCAGTTGTAATTGATACA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCGTTTCTATACtatat                           >  1:636584/1‑141 (MQ=255)

                                                                                                   cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt                        >  1:995682/1‑141 (MQ=255)

                                                                                                   cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt                        >  1:402834/1‑141 (MQ=255)

                                                                                                      tcatcaGTTGTAATTGATGCA‑AAAAAATTATCTAAAAAACTGCTGGTCTAACGTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                     <  1:225939/141‑1 (MQ=255)

                                                                                                        atcaGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCTGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt                   <  2:547838/141‑1 (MQ=255)

                                                                                                           aGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt                >  1:458101/1‑141 (MQ=255)

                                                                                                                tAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAt           <  2:420638/141‑1 (MQ=255)

                                                                                                                aaaTTAATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCCTTTCTATACTATATATTATTTTTCTAAAAt           <  2:421082/140‑1 (MQ=255)

                                                                                                                  aTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt         >  1:879334/1‑141 (MQ=255)

                                                                                                                    tGATGCAAAAAAA‑TTATCGCAAAAACTGACGAACAATCTTCAGATAAAAACAAATAATAACAAGGAGCTCGCTAGTACCTCTTAAACAGATGACGCTCGCAAACGCTTTTCTATACTATATATTATTTTTCAAAAATTTTa       >  2:291341/1‑141 (MQ=255)

                                                                                                                    tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTCTATGCTATATATTATTTTTCTAAAATTTTa       >  2:512871/1‑141 (MQ=255)

                                                                                                                    tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa       >  2:663411/1‑141 (MQ=255)

                                                                                                                    tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa       >  1:611684/1‑141 (MQ=255)

                                                                                                                    tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTGCTAAAATTTTa       >  1:149274/1‑141 (MQ=255)

                                                                                                                     gATGCAAAAAAATA‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTATCTCTTAAAATGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTaa      <  2:112330/141‑1 (MQ=255)

                                                                                                                     gATGCA‑AAAAAATTCTCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTaa      <  1:587285/141‑1 (MQ=255)

                                                                                                                          caaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGACGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAcc  <  1:689723/139‑1 (MQ=255)

                                                                                                                          caaaaaaaTTATCGAAAAAACTGAAGAACAATCTGCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAGGATGATGCTAGCATGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAcc  <  1:602643/139‑1 (MQ=255)

||

GTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC  >  CP000730/2455569‑2455831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`