Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,471	A→G	23.3%	S27S (AGT→AGC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,471	0	A	G	23.3%	84.5 / 26.3	61	S27S (AGT→AGC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (17/28);  new base G (6/8);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36331‑36611                                                                                                                                             |                                                                                                                                             cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                              >  1:301220/1‑141 (MQ=18) cGCCATTTGTAATAAGATTTTGTCTGCTTTTTCTTCCAAATTTTATATCAAATTGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                              <  1:33245/141‑1 (MQ=11)   ccATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGTTGCATATTCTTGAACCCCACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCCACTGTATCTCGATAGCt                                                                                                                                            >  2:134818/1‑141 (MQ=11)    cATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGACTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  1:266514/141‑1 (MQ=255)    cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:223568/141‑1 (MQ=11)    cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           >  2:48766/1‑141 (MQ=255)    cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  1:334411/141‑1 (MQ=18)    cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGCTGAATGAGGAACTCTTGAACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  1:94094/141‑1 (MQ=11)    cATTTGTAATAAGCTTTTGTGATCTTTTTCTTCCACATTTTATATAAATTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTCACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  2:92562/141‑1 (MQ=11)     aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                                                          >  1:241121/1‑141 (MQ=11)     aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                                                          >  1:276243/1‑141 (MQ=255)      tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                         <  2:170851/141‑1 (MQ=11)       ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAATGAATTCACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                        >  1:30691/1‑141 (MQ=11)       ttGTAATAAGCTTTTATATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACGCAACGGTAGATCGTTGAATAATGAACGTTTACACCACGTTATGTTAATATTTCAGGTAAATCACAATAACTCaaa                                                                                                                                        >  2:127527/1‑140 (MQ=11)       ttGTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAACGATGAACGTTTACACCACGTTCTCTTAATATTTCAGCTATATCACGATAGCTCAAt                                                                                                                                        <  2:1112/141‑1 (MQ=11)        tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAta                                                                                                                                       >  1:148163/1‑140 (MQ=11)        tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  1:196778/141‑1 (MQ=11)        tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  1:177782/141‑1 (MQ=18)         gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTATTGGCATATCACGATAACTCAATgc                                                                                                                                      <  1:395809/141‑2 (MQ=11)          taataaGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTTGGGCATATTTTTGAATCCAACGGTAGCTCGTTGACTTATGAACGTTTACACCACGTTCTCTTAATTTTTCTGATTTTTTTCGATATCTCATTgca                                                                                                                                     >  2:9769/1‑139 (MQ=2)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  1:68824/141‑3 (MQ=11)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  1:143348/141‑3 (MQ=11)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  2:186398/141‑3 (MQ=11)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     >  2:286614/1‑139 (MQ=11)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGCCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGCACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                     <  1:99486/141‑3 (MQ=255)          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                                                     >  2:38757/1‑141 (MQ=40)           aataaGCTTTTTTATTTTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcaa                                                                                                                                    >  2:250805/1‑138 (MQ=11)           aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                                                                                                                    <  2:120660/141‑4 (MQ=11)           aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAACGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcat                                                                                                                                    <  2:74768/141‑4 (MQ=9)           aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtat                                                                                                                                    >  2:264235/1‑141 (MQ=255)                    ttttATGCTTTTTCTTGCATGTTTGATACAAAATTGGGGCAAAGTCTTGAACCCAACGGTAGAGCGTTGAATGATGAAGGTTTACACCACGTCCCCGTAATATGTCAAGTATATCACGATACTTCaagtttcagcttatat                                                                                                                           >  2:264159/1‑127 (MQ=255)                    ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAt                                                                                                                           <  1:38757/141‑1 (MQ=35)                       tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                        >  2:299495/1‑141 (MQ=35)                          gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTCGAtagtatc                                                                                                                     >  2:1162/1‑139 (MQ=255)                               ttcttcCAAATTTGATACAAAATTGGGTCCTATTCTTGAACCCAACGTTATATCGTTGAATGATCAACTTTTACACCACGTTCCCTTAATATTTCAGATATATCACTATAACTCAATCTATATCTTAGATAGTAGCCAACg                                                                                                                <  2:111098/141‑1 (MQ=11)                                           tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAAGATAACTCAATGTATATCTTAGATAGTAGCCAAAGGCTACAGTGGTa                                                                                                    >  2:59037/1‑141 (MQ=255)                                             aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGAAGCCAACGGCTACAGTGATGAc                                                                                                  >  1:31455/1‑141 (MQ=21)                                                     ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                          <  1:39065/141‑1 (MQ=32)                                                     ttGGAGCATATTCTTGAACCTAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                          <  1:39095/141‑1 (MQ=12)                                                          gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                     <  1:264235/141‑1 (MQ=32)                                                                   cgAACCCAACGGTTGACCGTGGAATGAGGAACGTTTACACCACGATGCCATAATATTTCAGATATATCATAATAACTCAATGTATATCTTAGATAGTAGACAACGGCTACGGTGATAACATCCTTGTTAAAATGTTtataa                                                                            >  2:189242/2‑140 (MQ=11)                                                                       cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                                                        >  1:84376/1‑141 (MQ=32)                                                                        ccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaa                                                                       <  2:221418/141‑1 (MQ=32)                                                                           aCGGTAGATCGTTGAATGATGAACGTTTACACCACGGTCTCTTAATATTTCAGATATATCACGATAACTCCATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATCACGCCTTTATTATATTTTTTATAACTGACATa                                                                    >  2:60503/1‑141 (MQ=11)                                                                            cgttAGACCTTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                   <  2:356129/138‑1 (MQ=17)                                                                            cGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                   <  2:158661/141‑1 (MQ=32)                                                                            cGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                   <  2:218434/141‑1 (MQ=32)                                                                              gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                 <  1:230260/141‑1 (MQ=32)                                                                              gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                 >  1:379878/1‑141 (MQ=32)                                                                                aGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa                                                               <  1:332490/141‑1 (MQ=32)                                                                                aGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa                                                               <  1:76155/141‑1 (MQ=32)                                                                                         aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaaga                                                      <  1:1162/141‑1 (MQ=32)                                                                                          atgatgAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                     >  1:142964/1‑141 (MQ=32)                                                                                            gatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTc                                                   <  1:43733/141‑1 (MQ=32)                                                                                              tgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACACTGTTCATATCTGAAATAGTTCCTACAGAAGactact                                                 >  2:93103/1‑141 (MQ=11)                                                                                                     ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                          <  1:305376/141‑1 (MQ=32)                                                                                                             cGTTCCCTTAATATTTCAGATATATCACGATCACTCAATGTATATCTTAGCTAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAGATtat                                  <  2:180757/141‑1 (MQ=12)                                                                                                                       atagTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTCTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                        <  2:267572/141‑1 (MQ=21)                                                                                                                           ttCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATACTATAAATTCAAc                    <  2:283701/141‑1 (MQ=25)                                                                                                                                    atCACGATAACTCAATGAATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACa           <  2:17914/141‑1 (MQ=17)                                                                                                                                       aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa        >  2:148273/1‑141 (MQ=35)                                                                                                                                        cgaataCTCAATGAATTTTATAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc       <  1:377235/136‑1 (MQ=11)                                                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACCGTGATAACATCCTTGTTAATTTGTTTATATCTGAACTAGTTTATTTTGTTTACTCCTTTTTGTTAAATTTATATTATAAATTCAACTTTGCAAGAGAACCGt    >  2:231379/1‑141 (MQ=11)                                                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACACCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt    <  1:186800/141‑1 (MQ=37)                                                                                                                                            aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta   <  1:103414/141‑1 (MQ=37)                                                                                                                                             aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTAAACTTTGCAACAGAACGGtat  >  1:80346/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                             CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36331‑36611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑