Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 197,945 T→C 11.5% S324P (TCA→CCA)  USA300HOU_0186 → possible ABC superfamily ATP‑binding cassette transporter, binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730197,9450TC11.5% 95.4 / 2.8 43S324P (TCA→CCA) USA300HOU_0186possible ABC superfamily ATP‑binding cassette transporter, binding protein
Reads supporting (aligned to +/- strand):  ref base T (16/21);  new base C (3/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACATCCTATGGTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTAGGAAAAA  >  CP000730/197809‑198082
                                                                                                                                        |                                                                                                                                         
gataTCGTATGGTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATg                                                                                                                                       <  2:370995/138‑1 (MQ=255)
          ggTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCca                                                                                                                             <  1:100690/141‑1 (MQ=255)
            tGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATTGCGTTCATGACACGTCCcaca                                                                                                                           <  1:20839/141‑1 (MQ=255)
            tGATCTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCcaca                                                                                                                           <  1:10776/141‑1 (MQ=255)
              aTTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCACTTGTTTAATCCACCTGCATCTGATGCCTTTGTTGAACCGTCATTGTATAAGCATGCATCGCGTGGATTACGCGTCCCACaaa                                                                                                                         <  2:377594/141‑1 (MQ=255)
                ttAACAATTCAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGAACGCATCGCGTCCCTACCACGCCCCACAAATa                                                                                                                       >  2:189238/1‑141 (MQ=255)
                  aaCAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAAc                                                                                                                     >  1:61627/1‑141 (MQ=255)
                  aaCAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAAc                                                                                                                     >  1:214486/1‑141 (MQ=255)
                   aCAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACa                                                                                                                    <  1:274695/141‑1 (MQ=255)
                   aCAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCAGTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACa                                                                                                                    >  2:141586/1‑141 (MQ=255)
                       ttAAGCCATCCGGCTATCAAGAAATTACGACATTGGCAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAAtt                                                                                                                <  1:61380/141‑1 (MQ=255)
                       atAAGCCATCCGTCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAAtt                                                                                                                <  2:351472/140‑1 (MQ=255)
                        taggCCAGCCGGCTATCAAGAAATTACGACATGGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAAttt                                                                                                               <  1:305303/138‑1 (MQ=255)
                        tAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAATGAGGCATCGCGTCCATGACACGTCCCACAAATAACAAAttt                                                                                                               >  1:27637/1‑141 (MQ=255)
                        tAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTCCATGACACGTCCCACAAATAACAAAttt                                                                                                               >  1:27625/1‑141 (MQ=255)
                        tAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGACATCGCGTTCATGACACGTCCCACAAATCACAAAttt                                                                                                               >  2:119210/1‑141 (MQ=255)
                              aTCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATCAGGAAGCATCGCGTTCATGACACGTGCCCCAAATAACagaatgagattc                                                                                                         >  2:210565/1‑130 (MQ=255)
                                          aGAACTTACGACATTGGGAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATcaca                                                                                             <  1:137570/141‑1 (MQ=255)
                                                 accaCATTGGTAAAACAACATCATTTGTTTAATCCACCCGCATATGATGACTTTCTTGAACCGTCATTGTATAAGGAGGCAGCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATttatta                                                                                      <  2:8135/138‑1 (MQ=255)
                                                     cATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATTACACGTCCCACAAATAACAATTTTATATGACCTATTATCACATTTATTAtttt                                                                                  <  1:256132/141‑1 (MQ=255)
                                                     cATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTAtttt                                                                                  <  1:72947/141‑1 (MQ=255)
                                                     cATTGGTAAAACAACATCATTTGTTTAATCCAACTGACTACGTTGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTAtttt                                                                                  <  1:206799/141‑1 (MQ=255)
                                                                  acaTCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTgg                                                                     <  1:210731/141‑1 (MQ=255)
                                                                  acaTCATTTGTTTAATCCACCTGCATATGATGACTTTGGTAAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACCAATAACAAATTATAATTCCCTATTATCCCGCTTAATATTTTCTTGGGCATTTgg                                                                     >  1:279580/1‑141 (MQ=255)
                                                                     tcatTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGaa                                                                  <  1:165529/141‑1 (MQ=255)
                                                                      catTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCCCAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGaaa                                                                 >  2:369469/1‑141 (MQ=255)
                                                                                              gcgggcTTTGTTGAACCGTCATTGTATAAGGAGCCATCGTGTTCAAGACACGTCACACAGATAACAAGTTCATCTTACCTATTATCACAGTTATTATTTTCTTAGGCATGTGGGAAATGGTCAATATTATTGGGCATGAcc                                         <  2:58895/136‑1 (MQ=255)
                                                                                              gatgaCTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTCCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTAcc                                         <  2:27637/141‑1 (MQ=255)
                                                                                              gatgaCTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTCCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTAcc                                         <  2:27625/141‑1 (MQ=255)
                                                                                               atgaCTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCa                                        <  1:178761/141‑1 (MQ=255)
                                                                                                    tttGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAAGAAATTTATATTACCGATTATCACATTTATTATTTTCTTAGGCATCTGGGAAATGGTCATTATTATTGGGCATTACCAACCTg                                   <  2:262271/141‑1 (MQ=255)
                                                                                                          gAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGt                             <  2:154641/141‑1 (MQ=255)
                                                                                                                   ttGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGTGCATGACCAACCTGTATTGTTACCGGGTc                    >  2:317963/1‑141 (MQ=255)
                                                                                                                   ttGTATAAGGAGGCATCGCGTTCAGGACACGTCCCACTAATAACAAACTTATATTACGTATTATCACATTTATTATTGTCTTAGGCATTTCGGAAATGGTCATTATTATTGGGCATTACAAACCTGTATTGTTACCGGGTc                    >  2:95193/1‑141 (MQ=255)
                                                                                                                     gtctaAGGAGGCATCGCGTTCATGACCCGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCt                  <  2:15586/138‑1 (MQ=255)
                                                                                                                       ataAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGc                >  1:291026/1‑141 (MQ=255)
                                                                                                                              ggCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTa         <  1:141586/141‑1 (MQ=255)
                                                                                                                                    gcgTTCATGACACGTCCCCCAAATAACAAATTTATATTACCGATTATAACGTTTATTTTTTTCTTAGGCATTTGGGAAATGGTCATTATTCTTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGGTATTGTAGGaaaa   >  1:79295/1‑141 (MQ=255)
                                                                                                                                    gcgTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGGGCCGGTGAGTAGGATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTAGGcaaa   >  1:91842/1‑141 (MQ=255)
                                                                                                                                    gcgTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTTGGCATTACCAACCTGTATTGTTACCGGGTCGTGCTCTTGTAGGgaaa   >  1:86136/1‑141 (MQ=255)
                                                                                                                                    gcgTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTAGGaaaa   >  2:234836/1‑141 (MQ=255)
                                                                                                                                    gcgTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTGATTATTTTCTTAGGCATTTGGGAAATGGGCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTAGGaaaa   >  1:67154/1‑141 (MQ=255)
                                                                                                                                     cgTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATTACATTTATTATTTTCTTCGGCATTTGGGATATGGTCATTATTATTGGGCATTACCACACTGTCTTGTTACCGGGTCCTGCTGTTGTAGgaagaa  >  2:295448/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                         
GACATCCTATGGTGATTTAACAATTAAGCCATCCGGCTATCAAGAAATTACGACATTGGTAAAACAACATCATTTGTTTAATCCACCTGCATATGATGACTTTGTTGAACCGTCATTGTATAAGGAGGCATCGCGTTCATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTAGGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCTGCTCTTGTAGGAAAAA  >  CP000730/197809‑198082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: