Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4730CT100.0% 70.6 / ‑6.5 25A67T (GCT→ACT) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/10);  minor base G (0/1);  total (14/11)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATC  >  CP000730/1744336‑1744608
                                                                                                                                         |                                                                                                                                       
ttGACCTGGTTGTATTTCATCTTGTTGCATTGTTAAAACATTTGTACCATAGTCTATATCATACTCTTGACGCATGACTTCTTGGAGTCATTTCCCTTCTTTCCTATCAGGTTCAAAGTCAATACCCATTTAATAAGGTAc                                                                                                                                      <  2:20615/141‑1 (MQ=255)
  gACCTGGTTTAATTGCATCTTTGTGCATTGTTAACACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAg                                                                                                                                    <  2:89636/141‑1 (MQ=255)
       ggTTTATGTGTATCGTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAg                                                                                                                               <  2:236111/141‑1 (MQ=255)
                gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATa                                                                                                                      >  2:62022/1‑141 (MQ=255)
                gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATa                                                                                                                      >  2:62006/1‑141 (MQ=255)
                        gtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAAGTAATTTCCCTTCTTTTCTAACAGGTGCAAAACCCATCCCCATTGAATAAGTTACAGGACAGACAATGATCAAGCCAcg                                                                                                              >  1:75937/1‑141 (MQ=255)
                           cATTGTTAAAACATTTGTACCATCTTTTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                           >  2:183337/1‑141 (MQ=255)
                                    aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                                                  <  1:204808/141‑1 (MQ=255)
                                                atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc                                                                                      >  1:251701/1‑141 (MQ=255)
                                                    tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa                                                                                  >  1:56553/1‑141 (MQ=255)
                                                            aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                                          >  2:310527/1‑141 (MQ=255)
                                                                 cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                     <  1:62022/141‑1 (MQ=255)
                                                                 cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                     <  1:62006/141‑1 (MQ=255)
                                                                      ctaacgACTTCATGAGGAAATTTCCCTTCTTTTCTGACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                                                                <  1:350188/136‑1 (MQ=255)
                                                                                      gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAACCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                                                <  1:175897/141‑1 (MQ=255)
                                                                                         aTTCCCTTTCGTTTCTCGCAGGTGCAAGGCCATTCCCCCTTGAATAAGTTACAGGACAGCCAATGATAATGCCTCGCGCTTCAGGTCCTACTACGATATCAGCATCTCTGTCTTTTGCGTATTCTACAATTTTTTCTGTTg                                             <  2:137843/141‑1 (MQ=255)
                                                                                            tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAt                                          >  1:372651/1‑141 (MQ=255)
                                                                                                             ggTGCAAAGCCAATCTCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAGCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCa                         >  1:201254/1‑141 (MQ=255)
                                                                                                                gCAAAGCCAATCCCCTTTGAATAAGTTTCAGGACAGCCAATGTTAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACGATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                      <  2:107813/141‑1 (MQ=255)
                                                                                                                   aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                   >  2:213923/1‑141 (MQ=255)
                                                                                                                    aGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                  >  2:182236/1‑141 (MQ=255)
                                                                                                                            ttcATTGAATAAGTTACAGGATATCCAATGATAAAGCAACGCGCTTCAGGTCCTACAACTATATCAGCATCTCTGTCTTTTGCGTATTCTACAATTTGAGCGGGTGCATAGCCATATGCTTCACCATTATCCATAATTgta          <  2:75937/139‑1 (MQ=255)
                                                                                                                              cATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTCTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagt        >  1:157061/1‑141 (MQ=255)
                                                                                                                              cATTGAATAAATTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagt        >  2:269698/1‑141 (MQ=255)
                                                                                                                                    aTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATc  >  2:355681/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATC  >  CP000730/1744336‑1744608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: