Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 478,502 C→A D342E (GAC→GAA ndhF → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA99.4% 559.5 / NA 176D342E (GAC→GAAndhFNADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (86/88);  total (86/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTAG  >  CP000730/478357‑478647
                                                                                                                                                 |                                                                                                                                                  
ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                 <  2:32231/149‑1 (MQ=255)
ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                 <  1:62234/149‑1 (MQ=255)
ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                 <  1:584692/149‑1 (MQ=255)
ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                                 <  1:1261487/149‑1 (MQ=255)
  ttGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTa                                                                                                                                               <  1:1309216/148‑1 (MQ=255)
  aTGTTAGTTCAATGTGCATTGGTTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTa                                                                                                                                               <  2:1314878/149‑1 (MQ=255)
  aTGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTa                                                                                                                                               <  2:405446/149‑1 (MQ=255)
   tGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                              >  2:1339803/1‑149 (MQ=255)
     ttAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                            >  1:1137174/1‑149 (MQ=255)
       aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAGGCATCTGCTAAAGAAGCTTATGGCt                                                                                                                                          >  1:365472/1‑149 (MQ=255)
       aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCt                                                                                                                                          >  1:688003/1‑149 (MQ=255)
       aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCt                                                                                                                                          >  1:793677/1‑149 (MQ=255)
            aaTGTGCATTGGGTGTATATTCAACAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                                     >  1:1003138/1‑149 (MQ=255)
               gtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTc                                                                                                                                  <  1:1016407/149‑1 (MQ=255)
                tgCATTGGGTGTATATTCAGCAGCGATTGTTCATTTAATATTGCACGGTATTTTTAAAGCGACATTGTTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAAAAGCTTATGGCTGGATTGTCa                                                                                                                                 >  1:818463/1‑149 (MQ=255)
                tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTCAAGCAGCCTCTGGCTGGATtctcc                                                                                                                                 >  2:1308066/1‑148 (MQ=255)
                tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                                 >  1:1123325/1‑149 (MQ=255)
                tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                                 >  2:463174/1‑149 (MQ=255)
                 gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCAt                                                                                                                                >  2:1062072/1‑149 (MQ=255)
                 gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCAt                                                                                                                                >  1:242401/1‑149 (MQ=255)
                  cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                               <  2:39607/149‑1 (MQ=255)
                   aTTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATgg                                                                                                                              <  1:1084707/149‑1 (MQ=255)
                    ttGGGTGTATATTCAGCAGCGCTTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg                                                                                                                             >  1:1198569/1‑149 (MQ=255)
                       ggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                          <  1:957608/149‑1 (MQ=255)
                       ggTGTATATTCAGCAGCGATTATTCATTTAATATTGAACGGTACTTTGAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                          <  1:249057/149‑1 (MQ=255)
                         tgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                        <  1:370663/149‑1 (MQ=255)
                         tgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                        <  2:1133207/149‑1 (MQ=255)
                          gttaatTCAGCAGCGATCATTCATTTAATATTGACAGGTTTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGAGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtgt                                                                                                                       <  1:12197/145‑1 (MQ=255)
                          gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGGAGCTTATGGCTGGATTGTCATGGGACgggt                                                                                                                       >  2:409352/1‑149 (MQ=255)
                          gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtgt                                                                                                                       >  1:202918/1‑149 (MQ=255)
                           tataTTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCGGGATTGTCATGGGACGTGTa                                                                                                                      >  2:23237/1‑149 (MQ=255)
                                tCAGCATCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                 <  2:69191/149‑1 (MQ=255)
                                tCAGCAGCTATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTTTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                 <  2:35534/149‑1 (MQ=255)
                                tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAGGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGATGGATTGTCATGGGACGGGTGttgac                                                                                                                 >  1:598438/1‑146 (MQ=255)
                                tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                                 <  2:550766/149‑1 (MQ=255)
                                tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGc                                                                                                                 >  1:479597/1‑149 (MQ=255)
                                 caggaggtattattCATTTAATATTGCACGGTATTTTTAAATCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                                <  2:629723/141‑1 (MQ=255)
                                  agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                               >  1:1087083/1‑149 (MQ=255)
                                   gcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                              >  2:11221/1‑149 (MQ=255)
                                   gcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                              >  1:231623/1‑149 (MQ=255)
                                    cagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtatt                                                                                                             >  1:1055533/1‑149 (MQ=255)
                                      gcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGAGGTGTATTAGCtattat                                                                                                          >  2:943898/1‑149 (MQ=255)
                                      accccccatCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGCATTGTCATGGGACGTGTATTAGCtattat                                                                                                          >  2:889839/9‑149 (MQ=255)
                                        gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTCAAGAAGCTTATGGTTGGCTTGTCATGGGATGTGTCTTTGCTATTATCg                                                                                                        >  1:1027816/1‑149 (MQ=255)
                                           tattCATTTAATATTGCACGGTAGTTTGAAAGCAACATTATTTTTACAATGAGGTTCTATCGTGAAGCGATTCAATATTCCACAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTgg                                                                                                     >  1:1002630/1‑149 (MQ=255)
                                               cATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                                 >  1:1265814/1‑149 (MQ=255)
                                                 tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                               <  2:781437/149‑1 (MQ=255)
                                                 tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGATCTATCGTGAAGCGATTCGATATTCCAAAACAAGCATCTGCTAAAGAAGCTGATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtttt                                                                                               >  1:924787/1‑148 (MQ=255)
                                                  ttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                              >  1:1105038/1‑149 (MQ=255)
                                                     atcttGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtt                                                                                           <  2:1065027/146‑1 (MQ=255)
                                                      tatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                          <  2:591772/149‑1 (MQ=255)
                                                      tagagCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                          <  1:147751/145‑1 (MQ=255)
                                                         tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       <  1:500975/149‑1 (MQ=255)
                                                         tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       <  2:854831/149‑1 (MQ=255)
                                                         tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       >  1:257111/1‑149 (MQ=255)
                                                         tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                       >  2:224976/1‑149 (MQ=255)
                                                           cACGGTATTTTTAAAGCAGCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTCCTATTTTGGAt                                                                                     >  1:974376/1‑149 (MQ=255)
                                                           cACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                                     >  2:812609/1‑149 (MQ=255)
                                                           cACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAAGTATTATCGTGGCATTTCTGTTTTGGAt                                                                                     >  2:1258274/1‑149 (MQ=255)
                                                           cACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTAGCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                                     >  2:1155023/1‑149 (MQ=255)
                                                            aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTTTTAGCTATTCTCGTGGCATTTCTATTTTGGATg                                                                                    <  1:672210/149‑1 (MQ=255)
                                                             cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATGATCGTGGCGTTTCTATTTTGGTTGa                                                                                   >  1:166334/1‑149 (MQ=255)
                                                                 aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagtag                                                                               <  1:424767/149‑1 (MQ=255)
                                                                   ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                             <  1:939617/149‑1 (MQ=255)
                                                                   ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                             >  1:1010355/1‑149 (MQ=255)
                                                                   ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                             <  2:718480/149‑1 (MQ=255)
                                                                     tttttttatttttattTTTAAAATCAGGTTCTATAGTGAAGCGATTTACTATTCAAAAAAAAGAATCTGCTAAAGAAGCTTATGGTTGGATTGTAATGGGACGTGTATTAGCTATTATCGTGGCATTTATATTTTGGATGAGTAGTGag                                                                           >  2:1203134/12‑148 (MQ=255)
                                                                     ttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGAc                                                                           >  2:165894/1‑149 (MQ=255)
                                                                          gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCAAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAg                                                                      >  2:1009389/1‑149 (MQ=255)
                                                                            aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                                    <  2:374570/149‑1 (MQ=255)
                                                                            aaCATTATTTTTACAAGCAGTTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                                    <  2:1324754/149‑1 (MQ=255)
                                                                                ttattTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                                <  1:1307929/149‑1 (MQ=255)
                                                                                ttattTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                                <  2:1198569/149‑1 (MQ=255)
                                                                                    ttttACCATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                            <  2:101887/149‑1 (MQ=255)
                                                                                    ttttACAATCAGGTTCTATCGTGAAGCGTTTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                            >  1:872702/1‑149 (MQ=255)
                                                                                    ttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGAAGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                            >  1:1282565/1‑149 (MQ=255)
                                                                                     tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                           <  2:1087083/149‑1 (MQ=255)
                                                                                     tttACAATCAGGTTCTAGCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                           <  1:210826/149‑1 (MQ=255)
                                                                                       tACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGTTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                                         <  2:849330/149‑1 (MQ=255)
                                                                                       tACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                                         <  1:1050399/149‑1 (MQ=255)
                                                                                         cAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGCGTAGTGACAGAAGTGCCTCTGCAGTGtt                                                       >  2:249898/1‑149 (MQ=255)
                                                                                                  tCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGAACATGGATTAGCTCCAATTCGTGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                              <  1:643197/149‑1 (MQ=255)
                                                                                                      tCGTTAAGCGATTCAATCTTCCAAAACAAGCATCTGCTAAAGGAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  2:271741/149‑1 (MQ=255)
                                                                                                      tCGTGAAGCGATTCAATATTCCAAAACACGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  1:83837/149‑1 (MQ=255)
                                                                                                      tCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  1:192666/149‑1 (MQ=255)
                                                                                                      tCGTGAAGCGATTAAATATTCCAAAACGAGCCTCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAtt                                          <  1:722166/149‑1 (MQ=255)
                                                                                                       cGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                         <  1:1062072/149‑1 (MQ=255)
                                                                                                          gAAGCGATTCAATATTCCAAGAGAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAg                                      <  2:1214798/149‑1 (MQ=255)
                                                                                                          gAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATCGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGCGCATATGAAGTGTTAAGTGCACTCATTCTAg                                      <  2:961802/149‑1 (MQ=255)
                                                                                                              cGATTCAATATTCAAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATAAGAAGTGTTAAGTGCACTCATTCTAGGATg                                  >  2:605935/1‑149 (MQ=255)
                                                                                                                    aaTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCAtt                            <  1:1339803/149‑1 (MQ=255)
                                                                                                                     atatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACGGAAGTGCATATGAAGTGTTAGGTGCACTCATTATGGGATGCGCATTa                           >  2:322534/1‑149 (MQ=255)
                                                                                                                     atatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                           >  1:298773/1‑149 (MQ=255)
                                                                                                                     atatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                           >  1:725229/1‑149 (MQ=255)
                                                                                                                              aaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCt                  <  1:96611/149‑1 (MQ=255)
                                                                                                                              aaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCt                  <  1:1238609/149‑1 (MQ=255)
                                                                                                                                   gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGaa             >  2:1181447/1‑149 (MQ=255)
                                                                                                                                    cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt            >  1:1160932/1‑149 (MQ=255)
                                                                                                                                    cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTATATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTTTCTTGGGAt            >  1:516932/1‑149 (MQ=255)
                                                                                                                                              aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTAg  <  1:930334/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTAG  >  CP000730/478357‑478647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: