New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1205323	72 (0.810)	40 (0.630) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	10/194	6.3	43.6%	intergenic (+459/‑365)	yfiE/lspA	possible lactoylglutathione lyase/A8 family signal peptidase II
	?	CP000730	= 1443739	NA (NA)					intergenic (‑312/+41)	USA300HOU_1341/brnQ2	IS1272 transposase/LIVCS family branched chain amino acid:cation symporter
Rejected: Coverage evenness skew score above cutoff.

TCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAA  >  CP000730/1443623‑1443876                                                                                                                        |                                                                                                                                          tCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACTAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAAcc                                                                                                                   <  1:922543/141‑1 (MQ=17) tCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAAcc                                                                                                                   <  1:116663/141‑1 (MQ=32) tCGAAGACCGACGCCTTTACTGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAAcc                                                                                                                   <  1:574792/141‑1 (MQ=11)  cGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACct                                                                                                                  <  1:581345/141‑1 (MQ=32)    aaGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACctct                                                                                                                <  2:436598/141‑1 (MQ=34)     aGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCtt                                                                                                               >  1:337354/1‑141 (MQ=35)      gACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTtttt                                                                                                              >  2:622038/1‑141 (MQ=17)      gACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCttt                                                                                                              >  2:173529/1‑141 (MQ=35)           aGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTatat                                                                                                         <  1:637424/141‑1 (MQ=35)                gagaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTc                                                                                                    >  1:593469/1‑141 (MQ=39)                gagaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTc                                                                                                    >  1:593415/1‑141 (MQ=40)                 agaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCg                                                                                                   <  2:642337/141‑1 (MQ=40)                    cGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTa                                                                                                >  2:218852/1‑141 (MQ=255)                      gCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTAtt                                                                                              <  2:1071/141‑1 (MQ=255)                         cccTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCt                                                                                           >  2:187672/1‑141 (MQ=255)                          ccTAGGAAAGCGAGGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTg                                                                                          <  1:555753/141‑1 (MQ=255)                                 aaGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGatat                                                                                   >  1:400537/1‑141 (MQ=255)                                  aGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATAtt                                                                                  <  1:67392/141‑1 (MQ=255)                                        gCCATTCAATACGAAGTATCGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTg                                                                            >  2:869498/1‑141 (MQ=255)                                              cAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCtt                                                                      >  1:290757/1‑141 (MQ=255)                                              cAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCtt                                                                      >  1:872951/1‑141 (MQ=255)                                                aTACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGt                                                                    <  2:1015258/141‑1 (MQ=255)                                                  aCGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGttt                                                                  >  2:699200/1‑141 (MQ=255)                                                  aCGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGttt                                                                  >  2:340162/1‑141 (MQ=255)                                                   cGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGtttt                                                                 <  1:699200/141‑1 (MQ=255)                                                    ttcgTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTa                                                                >  2:194512/4‑141 (MQ=255)                                                     aaGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTAc                                                               <  1:828799/141‑1 (MQ=255)                                                         aTTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAat                                                           <  2:948080/141‑1 (MQ=255)                                                          ttGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAata                                                          <  2:305845/141‑1 (MQ=255)                                                           tGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAatat                                                         >  1:494765/1‑141 (MQ=255)                                                            gTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTATTGTTTTATGTTCCAACCTCTTTTATATTATTCGTTATTCCTTTTGTTATTTATTTGTATCTTGTTTTACTAatatt                                                        >  2:218063/1‑140 (MQ=18)                                                                aaaTAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGATTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGa                                                    >  2:768877/1‑141 (MQ=255)                                                                      agaACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGAt                                              >  2:949872/1‑141 (MQ=255)                                                                      agaACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGAt                                              >  1:52623/1‑141 (MQ=255)                                                                       gaACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATa                                             >  1:368807/1‑141 (MQ=255)                                                                         aCAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGc                                           <  1:512310/141‑1 (MQ=255)                                                                           agcagTAAGACA‑ATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCt                                         >  2:906623/1‑141 (MQ=37)                                                                           agcagTAAGA‑TAATTTTC‑AATT‑AGAAAATATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCt                                         >  1:893617/1‑141 (MQ=37)                                                                           agcagTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCt                                         >  2:633295/1‑141 (MQ=255)                                                                           agcagTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCt                                         <  2:442963/141‑1 (MQ=255)                                                                              agTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGTTTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCTACTAGATAGCATAat                                      >  2:547051/1‑141 (MQ=255)                                                                                         ttttCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCa                             >  1:784162/1‑141 (MQ=255)                                                                                          ttttcaattagAAAA‑TATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaa                            >  1:891849/11‑141 (MQ=38)                                                                                              tAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaataat                        >  1:104872/1‑141 (MQ=255)                                                                                                tttcagtaaTTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAAtt                       <  2:1009594/134‑1 (MQ=255)                                                                                                 tt‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGc                     <  2:917029/141‑1 (MQ=255)                                                                                                       aaTTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTg                >  2:946497/1‑141 (MQ=255)                                                                                                        aTTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTgg               >  1:471346/1‑141 (MQ=255)                                                                                                           aTCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCTGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTTGGaa            >  2:530205/1‑141 (MQ=255)                                                                                                           aTCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGaa            >  1:85988/1‑141 (MQ=255)                                                                                                           aTCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGaa            <  2:337354/141‑1 (MQ=255)                                                                                                            tCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAc           <  1:254418/141‑1 (MQ=255)                                                                                                            tCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAc           <  1:499331/141‑1 (MQ=255)                                                                                                            tCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAc           >  2:433942/1‑141 (MQ=255)                                                                                                             cTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAcc          >  1:712490/1‑141 (MQ=255)                                                                                                               tACTGCTGTTTTTTTAGGGATTTATTTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTGAATTGGATCTTGTTTTACAAATATTCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCAc        <  1:922589/141‑1 (MQ=255)                                                                                                                aCTGCTGTTTTTTTAGGGTTTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCAct       >  2:633239/1‑141 (MQ=255)                                                                                                                   gctgTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTa    <  2:494765/141‑1 (MQ=255)                                                                                                                     tgTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTaaa  <  2:1005303/141‑1 (MQ=255)                                                                                                                        |                                                                                                                                          TCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGA‑T‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAA  >  CP000730/1443623‑1443876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.