Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,232,052 G→T D233Y (GAT→TAT)  prpC → possible PP2C protein phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,0520GT94.0% 264.6 / 8.9 83D233Y (GAT→TAT) prpCpossible PP2C protein phosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (34/44);  total (35/48)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

ATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAT  >  CP000730/1231918‑1232187
                                                                                                                                      |                                                                                                                                       
atttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACg                                                                                                                                   <  2:42999/141‑1 (MQ=255)
 tttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATaaagc                                                                                                                                  <  2:574128/141‑4 (MQ=255)
       attaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACttt                                                                                                                            >  1:532380/1‑141 (MQ=255)
        ttaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTc                                                                                                                           <  2:893309/141‑1 (MQ=255)
        ttaAAGTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTGTGTTAGTAACAGAAGGTACAATAGAAGATCATGGTGATCAATTGATGCAATTGGCATTAGATACCCATTCGAAGTATAACGTTACCTTc                                                                                                                           <  2:170749/141‑1 (MQ=255)
          aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt                                                                                                                         >  2:603162/1‑141 (MQ=255)
           aaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATa                                                                                                                        >  2:681448/1‑141 (MQ=255)
            aTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATAc                                                                                                                       >  1:714469/1‑141 (MQ=255)
                aGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgc                                                                                                                   <  1:198353/141‑1 (MQ=255)
                 gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  >  2:99647/1‑141 (MQ=255)
                 gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  >  1:565739/1‑141 (MQ=255)
                 gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTcgcg                                                                                                                  <  1:753039/141‑1 (MQ=255)
                  aTGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 <  1:533460/141‑1 (MQ=255)
                   tGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGc                                                                                                                <  1:176717/141‑1 (MQ=255)
                   tGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGc                                                                                                                <  1:334442/141‑1 (MQ=255)
                    ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               >  2:504857/1‑141 (MQ=255)
                    ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               <  1:739440/141‑1 (MQ=255)
                    ggATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCt                                                                                                               >  1:272563/1‑141 (MQ=255)
                     gATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTACTGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTa                                                                                                              <  1:617223/141‑1 (MQ=255)
                     gATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTa                                                                                                              <  2:243489/141‑1 (MQ=255)
                     gATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTa                                                                                                              <  2:895364/141‑1 (MQ=255)
                          aCTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGaa                                                                                                         <  1:84274/141‑1 (MQ=255)
                          aCTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGaa                                                                                                         <  1:475973/141‑1 (MQ=255)
                            tGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAgg                                                                                                       <  1:628302/141‑1 (MQ=255)
                             gATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGt                                                                                                      <  2:463863/141‑1 (MQ=255)
                             gATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGt                                                                                                      >  2:1009456/1‑141 (MQ=255)
                                 aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  2:272563/141‑1 (MQ=255)
                                 aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  2:677881/141‑1 (MQ=255)
                                 aTGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATa                                                                                                  <  1:504857/141‑1 (MQ=255)
                                   gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGCAGGTGATaaa                                                                                                >  2:13053/1‑141 (MQ=255)
                                   gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa                                                                                                >  2:932600/1‑141 (MQ=255)
                                    ttAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAg                                                                                               >  1:777889/1‑141 (MQ=255)
                                     tAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGAGCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGt                                                                                              <  1:65863/141‑1 (MQ=255)
                                      aaaGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTa                                                                                             >  1:435674/1‑141 (MQ=255)
                                        aGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATg                                                                                           >  1:633642/1‑141 (MQ=255)
                                                  aTTAAGCGTTTGTTAGTAAAAGAAGGTAAAATAAAAGAACATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTaaaa                                                                                 <  2:464532/141‑1 (MQ=255)
                                                     aaGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataa                                                                              <  1:872767/141‑1 (MQ=255)
                                                      aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 >  1:552190/1‑105 (MQ=255)
                                                      aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg                                                                                                                 <  2:552190/105‑1 (MQ=255)
                                                      aGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataat                                                                             <  1:981789/141‑1 (MQ=255)
                                                               tAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACg                                                                    <  1:304023/141‑1 (MQ=255)
                                                                aGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGa                                                                   <  1:804656/141‑1 (MQ=255)
                                                                aGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGa                                                                   <  2:797339/141‑1 (MQ=255)
                                                                   aaaaGAAGGAACCACAGATGTTCATGGTACACCATCAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTAATTTCATACTCGCGGCTATTGAAGGTGCTATAGTCTGATAGGTAAACTAATACATGAACGatat                                                                <  2:616638/141‑1 (MQ=255)
                                                                    aaaGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGAtata                                                               <  2:364910/141‑1 (MQ=255)
                                                                      agaagGTACAATAGAAGATCATGGTGCTCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa                                                             >  2:730058/1‑141 (MQ=255)
                                                                      agaagGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa                                                             <  1:681448/141‑1 (MQ=255)
                                                                         agGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAAtt                                                          >  1:1025618/1‑141 (MQ=255)
                                                                                aTAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAATGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATa                                                   >  2:858740/1‑141 (MQ=255)
                                                                                aTAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTACATACTCGCGGCAATTGAAGGAGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATa                                                   >  2:591842/1‑141 (MQ=255)
                                                                                 tAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa                                                  >  1:291351/1‑141 (MQ=255)
                                                                                  agaagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAg                                                 >  1:899188/1‑141 (MQ=255)
                                                                                    aagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCt                                               <  2:661937/141‑1 (MQ=255)
                                                                                    aagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCt                                               <  1:450946/141‑1 (MQ=255)
                                                                                     agaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCtt                                              <  1:396195/141‑1 (MQ=255)
                                                                                      gaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTg                                             <  2:596407/141‑1 (MQ=255)
                                                                                      gaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTg                                             <  1:441396/141‑1 (MQ=255)
                                                                                        tCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGAAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggc                                           >  2:134863/1‑141 (MQ=255)
                                                                                          aTGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggcgg                                         <  2:758741/141‑1 (MQ=255)
                                                                                          aTGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggcgg                                         >  2:561167/1‑141 (MQ=255)
                                                                                          aTGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggcgg                                         <  1:215439/141‑1 (MQ=255)
                                                                                                    aTTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATg                               >  1:878952/1‑141 (MQ=255)
                                                                                                    aTTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATg                               >  1:878799/1‑141 (MQ=255)
                                                                                                     ttAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGa                              >  1:1008313/1‑141 (MQ=255)
                                                                                                      tAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAg                             <  1:274660/141‑1 (MQ=255)
                                                                                                      tAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAg                             >  1:518757/1‑141 (MQ=255)
                                                                                                       aaTGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGt                            <  2:260200/141‑1 (MQ=255)
                                                                                                         tGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTAc                          <  2:727396/141‑1 (MQ=255)
                                                                                                           cAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCg                        <  2:236730/141‑1 (MQ=255)
                                                                                                            aaTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGt                       >  1:108099/1‑141 (MQ=255)
                                                                                                            aaTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGt                       <  2:344725/141‑1 (MQ=255)
                                                                                                             aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      <  1:989732/141‑1 (MQ=255)
                                                                                                             aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      <  2:144260/141‑1 (MQ=255)
                                                                                                             aTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                      >  2:964619/1‑141 (MQ=255)
                                                                                                                 gCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATc                  <  1:297083/141‑1 (MQ=255)
                                                                                                                  cATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATTTGTTGTAAAATAATAAATAAACGATATAAAAGTTTAGATAAGCTGTGCGGCGGTGGCATGAGTACCGTTTATCt                 >  1:517202/1‑141 (MQ=255)
                                                                                                                  cATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCt                 >  1:412768/1‑141 (MQ=255)
                                                                                                                   aTTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCtt                <  2:1014584/141‑1 (MQ=255)
                                                                                                                          aaCCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAg         >  1:578341/1‑141 (MQ=255)
                                                                                                                          aaCCATACGAAATATAACGTTACTTTCATACTCGCGGCAATTGAAGGAGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAg         >  2:853003/1‑141 (MQ=255)
                                                                                                                            ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgat       >  1:365918/1‑141 (MQ=255)
                                                                                                                                tCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga   <  2:145696/141‑1 (MQ=255)
                                                                                                                                 cGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacgat  >  2:341403/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                       
ATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGAT  >  CP000730/1231918‑1232187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: