Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,486,381 G→T Q5215K (CAA→AAA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,486,3810GT100.0% 70.0 / NA 22Q5215K (CAA→AAA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/12);  total (10/12)

TAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGT  >  CP000730/1486244‑1486516
                                                                                                                                         |                                                                                                                                       
tAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTAtt                                                                                                                                      <  2:220137/141‑1 (MQ=255)
       ttCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCAATGTTTTATTAATGAtt                                                                                                                               <  2:53648/141‑1 (MQ=255)
            gttgttTTTTGTTGTTTTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCttt                                                                                                                          >  2:109795/1‑141 (MQ=255)
                   ttCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCa                                                                                                                   >  2:109915/1‑141 (MQ=255)
                                   cTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGt                                                                                                   <  2:149942/141‑1 (MQ=255)
                                     tGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTAc                                                                                                 <  1:387005/141‑1 (MQ=255)
                                        gCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTGAGTTGTCACTTGTGATGTTACTTGCGTAATAGGATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCg                                                                                              >  2:66978/1‑141 (MQ=255)
                                            aGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTAc                                                                                          >  1:351445/1‑141 (MQ=255)
                                                 cgcgCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGatta                                                                                     <  1:112429/141‑1 (MQ=255)
                                                                            gTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGa                                                          >  1:21653/1‑141 (MQ=255)
                                                                              tGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGAcc                                                        <  1:297528/141‑1 (MQ=255)
                                                                               gTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCt                                                       <  1:53725/141‑1 (MQ=255)
                                                                                 cACTTGTGATGTTACGGGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCGGc                                                     >  2:164791/1‑141 (MQ=255)
                                                                                                 tGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTACCTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCa                                     <  1:241632/141‑1 (MQ=255)
                                                                                                 tGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCa                                     <  1:424397/141‑1 (MQ=255)
                                                                                                  gCGTAATAGAATTTGGTTCCATCGGTGGGTTCGATGGTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCAt                                    >  2:84859/1‑141 (MQ=255)
                                                                                                               tGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTAtc                       >  1:125087/1‑141 (MQ=255)
                                                                                                                ggTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTAtct                      <  2:226076/141‑1 (MQ=255)
                                                                                                                   tCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCtt                   >  2:16578/1‑141 (MQ=255)
                                                                                                                      aTCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAAc                                                                                                              >  2:57593/1‑47 (MQ=255)
                                                                                                                      aTCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAAc                                                                                                              <  1:57593/47‑1 (MQ=255)
                                                                                                                                    tGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGt  <  2:124160/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGT  >  CP000730/1486244‑1486516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑