Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,672,590 G→T L135I (CTT→ATT)  rpoD ← DNA‑directed RNA polymerase sigma subunit RpoD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,672,5900GT100.0% 106.8 / NA 31L135I (CTT→ATT) rpoDDNA‑directed RNA polymerase sigma subunit RpoD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/12);  total (19/12)

TTGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAAGTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACAC  >  CP000730/1672457‑1672718
                                                                                                                                     |                                                                                                                                
ttGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGAt                                                                                                                           >  1:315087/1‑141 (MQ=255)
  gTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGAttt                                                                                                                         >  2:166874/1‑141 (MQ=255)
       aGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTa                                                                                                                    >  2:114529/1‑141 (MQ=255)
             aTTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTtcat                                                                                                              <  1:205526/141‑1 (MQ=255)
                     aCAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGt                                                                                                      >  1:408416/1‑141 (MQ=255)
                                      cATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTaa                                                                                     <  2:307534/141‑1 (MQ=255)
                                             ccTTCTTGGATTAAATCAAGGAATAGCATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGAtt                                                                              <  2:317108/141‑1 (MQ=255)
                                               ttcttGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATttc                                                                            <  1:429822/141‑1 (MQ=255)
                                                                      aCATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCTCTTAATAAGTTaa                                                     >  2:149175/1‑141 (MQ=255)
                                                                       cATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAac                                                    >  1:231958/1‑141 (MQ=255)
                                                                        aTACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAaca                                                   >  2:299534/1‑141 (MQ=255)
                                                                             aCGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGccc                                              >  2:431797/1‑141 (MQ=255)
                                                                                                 cAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGc                                                                                                                      <  2:355325/49‑1 (MQ=255)
                                                                                                 cAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGc                                                                                                                      >  1:355325/1‑49 (MQ=255)
                                                                                                     aCTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAAc                      <  1:399452/141‑1 (MQ=255)
                                                                                                         aCAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTggg                  <  2:434446/141‑1 (MQ=255)
                                                                                                          cgtttagttgTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCa                                             <  2:14495/104‑1 (MQ=255)
                                                                                                          cgtttagttgTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCa                                             >  1:14495/10‑113 (MQ=255)
                                                                                                          cAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGt                 >  1:40817/1‑141 (MQ=255)
                                                                                                           aaCTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTc                <  2:236621/141‑1 (MQ=255)
                                                                                                            aCTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCa               >  2:317762/1‑141 (MQ=255)
                                                                                                             cTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACACCCAATTTCTTTAAGGTACATACGAACTGGGTCat              <  2:409995/141‑1 (MQ=255)
                                                                                                                aaCGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCattta           >  2:279683/1‑141 (MQ=255)
                                                                                                                 aCGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCatttat          >  2:43390/1‑141 (MQ=255)
                                                                                                                 aCGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCatttat          >  1:238102/1‑141 (MQ=255)
                                                                                                                  cGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCCTCACCTTGTTCAATACGTTTGGCTAGTTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGTCCAATTTCTTTAAGGTACATACGAACTGGGTCatttatt         <  2:428931/141‑1 (MQ=255)
                                                                                                                    tAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTAtttt       >  1:402017/1‑141 (MQ=255)
                                                                                                                    tAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTAtttt       >  1:329005/1‑141 (MQ=255)
                                                                                                                         tCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGTTTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAATGTACATACTAACTGGGTCATTTATTTTAacac  >  2:292876/1‑141 (MQ=255)
                                                                                                                            cTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTaa                                                                                     <  2:395349/55‑1 (MQ=255)
                                                                                                                            cTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTaa                                                                                     >  1:395349/1‑55 (MQ=255)
                                                                                                                                     |                                                                                                                                
TTGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAAGTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACAC  >  CP000730/1672457‑1672718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑