| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,679,803 | G→T | G96V (GGA→GTA) | cbpD → | possible secretory antigen |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,679,803 | 0 | G | T | 97.3% | 113.7 / ‑4.8 | 37 | G96V (GGA→GTA) | cbpD | possible secretory antigen |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (17/19); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACT > CP000730/2679670‑2679937 | cGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTAtattat > 2:48865/1‑141 (MQ=255) cgcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACa < 2:300334/141‑1 (MQ=255) gcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACAt > 1:420858/1‑141 (MQ=255) aGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGc < 1:86976/141‑1 (MQ=255) gTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCa > 2:120829/1‑141 (MQ=255) ggTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgtt < 1:147117/141‑1 (MQ=255) gTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttg > 2:58024/1‑141 (MQ=255) aaaCCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAgttgtt < 1:222923/141‑1 (MQ=255) gTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACaa > 1:9209/1‑141 (MQ=255) cTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGtat < 1:120829/141‑1 (MQ=255) agagagGGGGGAGTGCTAAGAAGTGGGAGTACGATGCAGGTAGATCAAAATATGCGACTGGTCGTACACCAAGGAAATGTGGTGGCATGCAATCAACTGCAGTATATGGTGGACATGTCGCAGTTGTTGAACAAGtatata < 2:399796/139‑1 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg < 1:160814/141‑1 (MQ=255) gggCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg > 1:358015/1‑141 (MQ=255) gCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgta < 2:247861/141‑1 (MQ=255) tGCTAAAAATTGGTATTAAAATGAAAGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAAAAAAAATATAAAAACGGTAGTAt < 2:9209/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 1:194781/141‑1 (MQ=255) gCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAtt < 1:162889/141‑1 (MQ=255) aaaaTTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAg < 1:244768/141‑1 (MQ=255) ggTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAg < 1:330117/141‑1 (MQ=255) gTATTNCAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTGATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGa < 1:118460/141‑1 (MQ=255) aCAATGCACGTAAATCAAAATATGCGACTGGTCGTACACTAAGAAAAATTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTCTCTACAAACGGTAATATTAAAGTATCCGCATACa > 2:342609/1‑141 (MQ=255) cAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCCAGAAAATTAGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAACAACGGTAGTATCAAAGTCACAGAATACaa > 2:263925/1‑141 (MQ=255) tGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACtt > 2:174011/1‑141 (MQ=255) gCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACttt > 1:295894/1‑141 (MQ=255) cGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTAt > 2:224425/1‑141 (MQ=255) aTCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGccc > 1:407632/1‑141 (MQ=255) cAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCAt < 1:305385/141‑1 (MQ=255) cAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCAt < 2:257407/141‑1 (MQ=255) tCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACg < 1:58024/141‑1 (MQ=255) caacAAGAAAAATTGCTAGCAAGAAAGCAACTGAAATATATTATGGAAATATCGCAGTTGTTGAAAAAGTATATAAAAACGGAAGTATTAAAGTTTCAGAATACAAATTTTATCGCCCATTAACATTCAATACACgaataa < 2:341279/138‑6 (MQ=255) cAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTaa > 1:40685/1‑141 (MQ=255) cAAGAAAATTTGCTGTCATGCAATCAACTGCAGTATATTAAGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGAAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTaa > 2:59768/1‑141 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaaaa > 1:171508/1‑141 (MQ=255) cTGTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcag > 1:66766/1‑141 (MQ=255) gTCATGCAATCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGgcagca < 1:305591/141‑1 (MQ=255) tCAACTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACttt < 2:295894/141‑1 (MQ=255) cTGCAGTATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACt > 2:267628/1‑141 (MQ=255) | CGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACT > CP000730/2679670‑2679937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |