| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 1,232,052 | G→T | D233Y (GAT→TAT) | prpC → | possible PP2C protein phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,232,052 | 0 | G | T | 96.5% | 86.3 / ‑2.7 | 29 | D233Y (GAT→TAT) | prpC | possible PP2C protein phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (11/17); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACG > CP000730/1231917‑1232185 | tatttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAAc < 1:58614/141‑1 (MQ=255) aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt < 2:171678/141‑1 (MQ=255) aaaTTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCAt < 2:323815/141‑1 (MQ=255) gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTcgcg < 2:121662/141‑1 (MQ=255) aCTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGaa < 2:83232/141‑1 (MQ=255) aaTTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGAACATGGTGATCAATTAATGCCATTGGCATTAGATAACCATGCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAAGt < 2:172677/140‑1 (MQ=255) aTTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTg < 2:417698/141‑1 (MQ=255) ttATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGa < 1:132025/141‑1 (MQ=255) tATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGAt < 1:152546/141‑1 (MQ=255) gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa > 1:201347/1‑141 (MQ=255) gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATaaa > 1:315551/1‑141 (MQ=255) aaGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTAt > 1:131868/1‑141 (MQ=255) aCAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGAt < 2:410453/141‑1 (MQ=255) aTGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAgg > 2:418150/1‑141 (MQ=255) tAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaata < 2:228010/141‑1 (MQ=255) agaagGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaa > 2:182601/1‑141 (MQ=255) aTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTgg < 1:418150/141‑1 (MQ=255) tCATGGTGATCAATTAATGCAATTGGCATTTGATAACCATTCGAAATATAACGTTACTTTCATTCTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGGTATAAAATTGTAGATAAGCTTggc > 2:378379/1‑141 (MQ=255) ggTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGgcggcg < 1:419092/141‑1 (MQ=255) tGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTAc > 1:351119/1‑141 (MQ=255) tGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTa < 1:52128/141‑1 (MQ=255) tAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGTTGATAAAGTATGATAAGTAAAATAATACATGAACGATATAAAATCGTAGATAAGCTTGGAGAAGGTAAAATGAGTAACGTCTATCTTGc > 2:194057/1‑141 (MQ=255) aGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGATATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAATGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCt < 2:438051/141‑1 (MQ=255) tAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGaa > 2:247953/1‑141 (MQ=255) ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgat < 2:339593/141‑1 (MQ=255) ccATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGCTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTAGCATGAGTACCGTTTATCTTGCTGAAgat > 2:412431/1‑141 (MQ=255) ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa < 1:144911/95‑1 (MQ=255) ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATaa > 2:144911/1‑95 (MQ=255) ttCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacg < 1:26883/141‑1 (MQ=255) | TATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACG > CP000730/1231917‑1232185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |