BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	86.4%	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	86.4%	1306.7 / 196.6	724	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (59/40); new base T (307/311); total (372/352)
Fisher's exact test for biased strand distribution p-value = 8.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 726 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36238‑36517

  cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                                                                                           >  2:529841/1‑141 (MQ=255)

   aaCCATATATCTAAGGGATCGCCCTCTCCACAATTGGCACGGTATAAATAGCGCCATTTTCCTTTTATTTTGCTGTACGTCTCACCCATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATaaa                                                                                                                                          <  2:807856/141‑1 (MQ=11)

    aCCATATAGTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCTTTTTCCTTTTATTTTGATATATGTCTCATCAATACGCCATTTGTAATAAGCTTGTTTATGTTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                         <  2:153634/141‑1 (MQ=11)

        aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTgg                                                                                                                                     >  2:379839/1‑141 (MQ=255)

           atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                  >  2:668125/1‑141 (MQ=255)

            tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCa                                                                                                                                 >  2:55975/1‑141 (MQ=255)

            tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCACTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                                 >  2:594562/1‑141 (MQ=255)

             cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                <  1:165828/141‑1 (MQ=255)

             cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                <  1:361957/141‑1 (MQ=37)

              tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                               <  1:40838/141‑1 (MQ=37)

                atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                             <  2:497087/141‑1 (MQ=25)

                   ggtgtCGCTTTGCATCAATGGCACGAGTTAAATTGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCtt                                                                                                                          <  2:225195/139‑1 (MQ=14)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTTTTg                                                                                                                         >  2:76456/1‑141 (MQ=11)

                    atgtCCCTCTGGATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                         <  2:681963/141‑1 (MQ=11)

                    atgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTg                                                                                                                         >  2:702575/1‑141 (MQ=255)

                     tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATGTTATATAAAATTGGGGCATATTCTTGa                                                                                                                        <  2:602669/141‑1 (MQ=11)

                      gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCAATGGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                       >  1:663518/1‑141 (MQ=11)

                        cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAAcc                                                                                                                     >  2:24977/1‑141 (MQ=11)

                        cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAAcc                                                                                                                     >  1:190743/1‑141 (MQ=11)

                        cccTCTGCATCAATGGCACGAAATAAATAGCTACATTTTCCTTTTATTATGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATTACATAGCGGCGTATTCTTGAAcc                                                                                                                     >  2:27131/1‑141 (MQ=11)

                         ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCTTGAAccc                                                                                                                    >  2:516713/1‑141 (MQ=255)

                         ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                    <  1:442640/141‑1 (MQ=25)

                           tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCCGATTATTGAActaaa                                                                                                                  >  1:578735/1‑137 (MQ=11)

                           tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                  >  2:312540/1‑141 (MQ=21)

                                aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTGAGTTTCATATCCGGCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                             <  1:803457/141‑1 (MQ=11)

                                 tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                            <  1:707751/141‑1 (MQ=21)

                                  cAATGGCACCATATCAATAGCTCCATTTGCCTTTTATTATAATGTACGTCCCATCCAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTACATCCAAATTTAATCTAAAATTCAGGCACATTTTTGATCCCAACGGTAGa                                                                                                           <  2:419399/141‑1 (MQ=255)

                                   aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAACATTGGGGCACATTCTTGAACCCAACGGTATAc                                                                                                          >  1:717947/1‑141 (MQ=255)

                                   aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                                                                                          >  2:735197/1‑140 (MQ=255)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                         >  2:32512/1‑141 (MQ=21)

                                      ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGAGATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                       >  2:432508/1‑141 (MQ=11)

                                       gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                                                                                      >  2:627987/1‑141 (MQ=40)

                                       gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                      >  1:11288/1‑141 (MQ=255)

                                        cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                     >  1:743567/1‑141 (MQ=21)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                    >  1:791822/1‑141 (MQ=255)

                                           gATATAAATAGCTCCATGTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAATATTGGGGCGTTTTCTTGAACCCAACGGTGGATCGTTGAat                                                                                                  >  2:839045/1‑141 (MQ=11)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                 <  1:266195/141‑1 (MQ=255)

                                             tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                >  1:327265/1‑141 (MQ=21)

                                             aatagatagCACCATGTTCCTTTTTTTATGATGTACGTATCATCAAGTCGACATTTGTAATAGGCTTTTTTATGCTTTTTCCTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGACCGGTGAatga                                                                                                <  2:105500/140‑1 (MQ=11)

                                              ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAATTAGGGTCAGATTCTTGACCCCAACGGTAgctcgattgctaat                                                                                               >  2:751037/1‑128 (MQ=11)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                              <  1:502498/141‑1 (MQ=18)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACGCAAGGGTAGAGCGTTGAatggtg                                                                                              >  1:233923/1‑141 (MQ=11)

                                                aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                             <  1:731494/141‑1 (MQ=255)

                                                        ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGACCGTGGAatagtgacagattac                                                                                     >  1:824671/1‑128 (MQ=11)

                                                        ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGCGTTGTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAGCGGTAGATCGTTGTATGATGTACTATTac                                                                                     <  2:240970/141‑1 (MQ=11)

                                                         cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacc                                                                                    >  1:96143/1‑140 (MQ=21)

                                                         cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                    <  2:397318/141‑1 (MQ=21)

                                                         cATTTTCCTTTTATTTTGATGTACCTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTaca                                                                                    <  1:246727/141‑1 (MQ=255)

                                                           ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                  <  2:360553/141‑1 (MQ=255)

                                                             ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTATTATGCTTTTTCTTCCAAATTTGATACAAAATTAGGGCATAGTCTTGAACCCACCGGTAGACCGTTGAAGGAGAAACGTTTAcaccac                                                                                >  2:471817/1‑141 (MQ=11)

                                                             ttCCTTTTATTTTGATATACGTCTTATCAATACGTCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                <  1:876901/141‑1 (MQ=11)

                                                              tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                               >  1:15216/1‑141 (MQ=18)

                                                              tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                               >  1:557581/1‑141 (MQ=39)

                                                              tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                               <  2:718944/141‑1 (MQ=255)

                                                              tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACg                                                                               >  2:908894/1‑141 (MQ=11)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGGGCCCTTCGGTGAATCGTTGAATGATGAACGTTTACACCACGt                                                                              >  1:471017/1‑141 (MQ=11)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                              >  2:127082/1‑141 (MQ=255)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                              >  1:303434/1‑141 (MQ=255)

                                                               ccTTTTAGTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                              <  2:821039/141‑1 (MQ=255)

                                                                tttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                             <  2:635311/140‑1 (MQ=255)

                                                                 ttttCTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                            <  2:691773/141‑1 (MQ=255)

                                                                  tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                           >  1:691498/1‑140 (MQ=255)

                                                                   ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                          >  1:835493/1‑141 (MQ=40)

                                                                    tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATACATTCTCCTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCCACCACGTTctct                                                                         >  2:782041/1‑141 (MQ=11)

                                                                     tttttTATGTACGGCTCATCAATACGCCATTTGTAATAAGGTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTTGGGGCATATTCTTGAACCCCACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                        <  2:19966/140‑1 (MQ=11)

                                                                     aTTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                        <  1:204866/141‑1 (MQ=18)

                                                                     aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  1:754204/141‑1 (MQ=11)

                                                                          gatataagTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGGTCTCTTAatat                                                                   >  2:572045/8‑141 (MQ=11)

                                                                             gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                <  1:867258/141‑1 (MQ=18)

                                                                             atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                <  1:645312/140‑1 (MQ=255)

                                                                              tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                               >  2:189132/1‑141 (MQ=255)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                              >  2:890361/1‑141 (MQ=255)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAg                                                              >  1:357056/1‑141 (MQ=25)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                              >  1:766076/1‑141 (MQ=40)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTcggt                                                             >  1:904303/1‑138 (MQ=21)

                                                                                   ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                          >  1:393092/1‑141 (MQ=25)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGACTGATGAACGTTTCCACCAGGTTCTCTTAATATTTCAGATATATCa                                                      >  1:851523/1‑141 (MQ=11)

                                                                                        caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                     >  2:656972/1‑141 (MQ=21)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                  >  1:77912/1‑141 (MQ=255)

                                                                                            aCGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGGAGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCACATATTTTACGAtt                                                 >  2:344215/1‑140 (MQ=11)

                                                                                              gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                               >  1:377718/1‑139 (MQ=255)

                                                                                              cccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                               <  2:852943/140‑3 (MQ=255)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTGGAATGATGAGCGTTTACACCAGGTTCTCTTAATATTTCTGATATATAACGTTaagc                                              >  1:101536/1‑139 (MQ=11)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagct                                              <  1:348262/141‑4 (MQ=255)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                              >  1:177978/1‑141 (MQ=40)

                                                                                                 aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                            <  2:351642/141‑1 (MQ=18)

                                                                                                   gggggataaGCTTTTTTATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                          <  2:446087/136‑1 (MQ=255)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATCGCTCAATg                                         >  1:682807/1‑141 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATGTTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                         >  1:604907/1‑141 (MQ=255)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTGCTCTTAATATTTCAGATATATCACGATAGCGCAATgca                                       <  1:136401/141‑3 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                       <  1:433219/141‑3 (MQ=12)

                                                                                                      taataaGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                       >  2:294041/1‑139 (MQ=11)

                                                                                                      taataaGCTTTTTTACTCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                       <  1:892329/141‑3 (MQ=11)

                                                                                                       aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                      <  1:532942/141‑4 (MQ=11)

                                                                                                       aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                      <  1:31418/141‑4 (MQ=11)

                                                                                                       aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATTTATCTCGATAATTCAATTtat                                      >  2:259786/1‑141 (MQ=255)

                                                                                                                     tGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATGGCACTCTAACTCAATGTATATCTTAGAtagtag                        >  2:874052/1‑141 (MQ=255)

                                                                                                                                     tttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGa        <  2:316745/141‑1 (MQ=34)

                                                                                                                                      ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt       <  1:628033/141‑1 (MQ=35)

                                                                                                                                          tACAAAATTGGGGCATAGTCTTGAACCCAACGGTAGAAAGTTGAATGATGAACGTTTACAACACGTTCCCTTAATATTTCAGATATATCACGATAAATCAATGTATATCTTACATAGTAGCCACCGGCTACAGTGCTAACa   >  2:160299/1‑141 (MQ=11)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36238‑36517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`