Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 622,479 T→C 9.5% D1014D (GAT→GAC sdrE → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730622,4790TC 9.5% 262.4 / 2.0 122D1014D (GAT→GACsdrESer‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE
Reads supporting (aligned to +/- strand):  ref base T (59/44);  new base C (4/10);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 5.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAT  >  CP000730/622339‑622611
                                                                                                                                             |                                                                                                                                    
gattccgaTAGCCTCTCAGATTCCCATAGAGAACCTGATTCGAACAGCGATTCAGACTCAGATAGCGACTCAGCTTTCGATAGCGGTTCTATATTCATCTGCGACAAAGTTTCAGATAGAGATTCGGACTCAGACAGCGAt                                                                                                                                      <  2:328026/141‑1 (MQ=9)
gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                      <  1:462581/141‑1 (MQ=255)
 atgcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTAGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAtt                                                                                                                                     <  2:167003/138‑1 (MQ=255)
  ttcagaCAGCGACTCAGCTTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCCGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                    <  2:535125/141‑1 (MQ=255)
  ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                    <  1:53323/141‑1 (MQ=255)
     agaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGa                                                                                                                                 <  1:88588/141‑1 (MQ=255)
      gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                                >  2:909777/1‑141 (MQ=255)
       aCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAGTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGAATCAGACTCAGACAGCGATTCCGAca                                                                                                                               >  2:468152/1‑140 (MQ=14)
        cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTTAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                                                                              <  2:623553/141‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                           <  1:256099/141‑1 (MQ=255)
             aCTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATa                                                                                                                         >  1:799309/1‑141 (MQ=255)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGAGTCTGGAGCCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCCGATTCAGATAGCGATTCGGACTCAGAAAGAGAATCAGCCTCAGATAGCg                                                                                                                      >  1:176328/1‑141 (MQ=14)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                      >  1:281808/1‑141 (MQ=255)
                  gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                    <  1:719764/141‑1 (MQ=255)
                  aattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                    <  1:719773/140‑1 (MQ=255)
                   attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                                   <  1:570795/141‑1 (MQ=255)
                      cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                                <  1:567349/141‑1 (MQ=255)
                        gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCgacccagac                                                                                                              >  2:111684/1‑141 (MQ=255)
                        gatAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                              >  1:501681/1‑141 (MQ=255)
                              gACTCAGATCCGGACAGCGATTCAGACTCAGATAGCGACTCTGATGCTGACACCGACACCGACACAGACACCGCCTCAGATTCACATACCGATCCCGACGCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                        <  2:756874/141‑1 (MQ=17)
                                   aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                                   <  1:485092/141‑1 (MQ=255)
                                    gatttcgACTGCGACTCAGACTCCGCAATGCACTCACATGCAGATAGCGATTCAGACTCAGCCAGCGTCTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  <  2:709939/135‑1 (MQ=11)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  >  1:18799/1‑141 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  >  1:865456/1‑141 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTACGCCAGCGAc                                                                                                  >  1:261194/1‑141 (MQ=255)
                                    gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGAGATTCGGACTCGGACAGCGGCGCAGACTCAGAAAGCGACTCCGACCCAGACacagac                                                                                                  >  2:147971/1‑136 (MQ=14)
                                    cactCGAACAGCGATTCAGACTCAGACACCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                                  <  2:839805/138‑1 (MQ=14)
                                      ttCGGACAGCGATTCACACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAATCGGACTCAAACAACGAcca                                                                                                >  1:540755/1‑139 (MQ=14)
                                        cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAGAGCGACTCGGATTGAGAGAGCGACTGGGACTCAGAAGGCGATTGAGACCGAGATAGCGACTCAGACTGAGACGGCGCCgcag                                                                                              >  2:80551/1‑141 (MQ=14)
                                          gACAGCGATTCAGACTCAGATAGTGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                            >  1:324832/1‑141 (MQ=255)
                                          gACAGCGATTCAGACTCAGATAGCGACCCAGATTCCGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                            <  1:750480/141‑1 (MQ=255)
                                             aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattca                                                                                         <  1:789420/141‑1 (MQ=255)
                                             aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGCGACtcagacgaa                                                                                         >  2:540435/1‑137 (MQ=255)
                                                gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                                                                                      >  1:563605/1‑141 (MQ=255)
                                                 aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAAtcggattcaggaa                                                                                     >  2:14914/1‑138 (MQ=255)
                                                 aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACtcagattcagaaa                                                                                     >  2:724375/1‑139 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTTAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                   <  2:499160/141‑1 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCACACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                   <  2:584345/141‑1 (MQ=255)
                                                        cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                              >  1:803021/1‑141 (MQ=255)
                                                          cAGATAGCGACTCAGATTCAGATAGCGATTAAGATTCATACAGCGAATCCGACTCAGGAATCAAATCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCGGACAGCGACTCAGATTCAGAAAGCGGTTAgg                                                                            >  2:414440/1‑141 (MQ=11)
                                                          aagagagCGATTCAGATTCAGATAGCGCTTCAGACTCACACTGCGACTCAGATTCAGAAAGCGATTCAGACTCAGACAGCGAGTCAGAATCAGATATCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                            <  2:426589/140‑1 (MQ=11)
                                                            gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                          >  1:509428/1‑141 (MQ=255)
                                                            gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAACGACTCAGATTCAGATAGCCACTCGGag                                                                          >  2:768522/1‑140 (MQ=255)
                                                             aTAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCCGACTCAGACAGCGACCCAGCCTCAGATAGCGACTCgggac                                                                         >  2:5196/1‑138 (MQ=14)
                                                             aTAGCGACTCAGATTCAGATAGCGAGTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGCTCGCGATTCGGCCt                                                                         >  1:219242/1‑141 (MQ=255)
                                                               aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGCTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACCAACCCAGATTCAGATAGCGATTCAGACTCa                                                                       >  2:38923/1‑141 (MQ=14)
                                                               aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGACAGCGATTCAGACTCCGAAAGCGACTAAGACTCAGACAGCGACTCAGATGCAGATAGCGATTGAGACTcc                                                                       >  2:39031/1‑140 (MQ=14)
                                                               aGCCACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTAAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                       <  1:804167/141‑1 (MQ=255)
                                                                gccgcgccgattcagatACCGATTCAGACTCAGACCGCTACTCAGATTCAGATAGCGATTCGGACTCACACCGCCATTCAGACTTAGATCGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                      <  1:355100/133‑1 (MQ=14)
                                                                gCGACTCAGATTCAGATAGTAATTCAGACTCAGACAGCGACTCAGATTCAGATAACGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCGAGACAGCGACTCAGATTCAGATATCGATTCGGACTCAg                                                                      >  1:590858/1‑141 (MQ=14)
                                                                gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                      >  1:767534/1‑141 (MQ=255)
                                                                gCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                      <  1:150021/141‑1 (MQ=255)
                                                                  gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                    <  1:468152/141‑1 (MQ=255)
                                                                      cagattcagatAGCGACTCAGACTCAGACATCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                <  2:579669/141‑1 (MQ=255)
                                                                      cacattccgatAGCGATTCAGCCCCAGACAGCGACTCAGATCCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                <  2:221467/138‑1 (MQ=17)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGAAGTCATATAGCGAcc                                                             >  1:189007/1‑140 (MQ=255)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACACCGAcc                                                             >  2:310967/1‑140 (MQ=11)
                                                                              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAt                                                        >  1:133794/1‑141 (MQ=255)
                                                                                tacccaTTCAGACTCAGACACCGACTCAGATTCTGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                      <  2:887646/136‑1 (MQ=11)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                <  1:528437/141‑1 (MQ=255)
                                                                                        tgtggcgcagccagcGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                               <  2:134330/134‑1 (MQ=14)
                                                                                          agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                             <  1:160052/141‑1 (MQ=255)
                                                                                          agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAATCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                             <  2:563168/141‑1 (MQ=255)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAACGAt                                            >  1:368336/1‑141 (MQ=255)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGACAGCggt                                            >  1:319346/1‑139 (MQ=255)
                                                                                           gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGac                                            >  2:904992/1‑140 (MQ=255)
                                                                                            caccagacAGCGACTCAGATTCAGCTAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCAAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAACGACTCAGCAGCAGACAgagact                                           >  2:838552/4‑139 (MQ=14)
                                                                                              ccatacAGCGACTCAGATTCAGACAGCGATTCGCACTCAGACAGCGACTCAGACTCAGATAGCGACTCCGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCATATAGCGACGCAGAGTCAGACAGCGATTCa                                         <  2:180124/137‑1 (MQ=14)
                                                                                              ccagacAGCGACTCAGACTCAGACAGCGACTCGGATTCTGCGAGCGAGTCATACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCCGACAGCGATTCa                                         <  2:189281/140‑1 (MQ=14)
                                                                                                 cacacCGACTCAGATTCAGATAGCGATTCTGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                      <  2:69128/140‑1 (MQ=255)
                                                                                                        acgccgattccggtAGGGATTCGGAATCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                               <  2:16637/136‑1 (MQ=17)
                                                                                                          tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCTGATAGCGACTCAGAGTCCGACAGCGAGTGAGACTcgggtggg                             >  2:48333/1‑134 (MQ=17)
                                                                                                          tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAgg                             >  2:840738/1‑140 (MQ=255)
                                                                                                           cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                            >  1:326262/1‑141 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGGCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      >  1:473029/1‑141 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      <  1:493556/141‑1 (MQ=255)
                                                                                                                 cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                      >  1:473084/1‑141 (MQ=255)
                                                                                                                    atAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagacc                   >  1:849567/1‑140 (MQ=255)
                                                                                                                     tAGAGATCCCGACTCAGACAGCCACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                  <  2:766129/141‑1 (MQ=17)
                                                                                                                     aaGCGACTCCGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                  <  2:260458/140‑1 (MQ=255)
                                                                                                                        cGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga               >  1:340841/1‑141 (MQ=255)
                                                                                                                           ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGATAGCGACTCAGATTCAGAAAGCGATACAGACTCAGATAGCGACTcagacacagacaa            >  2:659819/1‑140 (MQ=255)
                                                                                                                            tctgccgcAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCCCACAGCGATTCAGACTCAGATAGCCACTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt           <  2:902145/134‑1 (MQ=11)
                                                                                                                            tcggtctctgcgaGCGCTTCAGACTCAGATAGCGACTCGCACTCCAACCGCATCTCGGTTTCAGAAATCGATTCCAACTAAGATAGCGACTCAGATTCTGACAGCAATTCAGACTCAGATAGCGACTCAGACCCAGACAGt           <  2:281808/129‑1 (MQ=9)
                                                                                                                            tcagtcTCAGACAGCAATTGAGACTCACACCGCGACCCAGACCCAGACAGCCACTCAGAGTCAGAGAGCGACTCGGACTCAGACAGCGACTCGGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt           <  2:226632/136‑1 (MQ=14)
                                                                                                                             cGGCCTCAGACAGCTACTCCGACTCAGACAGCGACTCCGACTCAGACAGCGACTCCGATTCAGGAAGCGATTCCGACCCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  2:431366/141‑1 (MQ=14)
                                                                                                                             cGGCCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  1:105024/141‑1 (MQ=255)
                                                                                                                             cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCCAGTg          >  1:364966/1‑141 (MQ=255)
                                                                                                                             cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg          <  1:882917/141‑1 (MQ=255)
                                                                                                                              ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGTGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga         <  1:220940/141‑1 (MQ=255)
                                                                                                                               gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACGGCGACTCAGAGTGTGATAGCGATTCGGACTCAGATAGCGCATCAGATTCGGACAGCGATTCGGATTCTGGTAGCGGGTGAGACGTAGAGAGTggt        >  2:148582/1‑139 (MQ=14)
                                                                                                                               gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat        >  1:105261/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt       >  1:913874/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACACAGACAGTgatt       >  2:438689/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAAACCCCGACAGTgatc       >  2:161650/1‑140 (MQ=255)
                                                                                                                                aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCgatt       >  2:487290/1‑141 (MQ=255)
                                                                                                                                aCTCAGACAGCGATACAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt       >  1:486959/1‑141 (MQ=255)
                                                                                                                                  tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca     >  2:764695/1‑141 (MQ=255)
                                                                                                                                    aGACTGCTACTCAGACGCAGACAGCGAGTCACCCTCAGCCGGCGACTGAGATTCTGATAGCGATTCGGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga   <  2:512896/141‑1 (MQ=14)
                                                                                                                                     gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACTgattcagat  >  1:905033/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                    
GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAT  >  CP000730/622339‑622611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: