Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,617,132	C→T	5.6%	L212L (CTC→CTT)	yqkF →	dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,617,132	0	C	T	5.6%	227.6 / 2.4	78	L212L (CTC→CTT)	yqkF	dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (45/27);  new base T (2/2);  total (49/29)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

AACGATAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTGGTT  >  CP000730/1616994‑1617268                                                                                                                                           |                                                                                                                                         aaCGATAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCCTTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCgc                                                                                                                                        >  1:464606/1‑140 (MQ=255) aaCGATAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTGGATGCACTTCAGAACAATGTTGTTAACGTGTTGGCAAGAGGACCTGTGTGTAAAGGATTATTAACTTCAGACAGTGTTGATGTGCTCgg                                                                                                                                        >  2:7078/1‑140 (MQ=255) aaCGATAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGa                                                                                                                                        >  2:226920/1‑141 (MQ=255)     aTAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAAt                                                                                                                                    >  1:571536/1‑141 (MQ=255)      tAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATa                                                                                                                                   <  1:18731/141‑1 (MQ=255)          ttctcAATTCAATTTGATTGATAATCGTCCAGTATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAAtt                                                                                                                               <  1:772291/140‑1 (MQ=255)          gTCTCAATTCAATTTGATTGATAATCCTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTGGACAATAAAtt                                                                                                                               >  2:564956/1‑141 (MQ=255)           tctcAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAAttt                                                                                                                              >  1:121753/1‑141 (MQ=255)            ctcAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTTGACAATAAATTTa                                                                                                                             >  1:103863/1‑141 (MQ=255)            ctcAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTTGACAATAAATTTa                                                                                                                             >  1:342209/1‑141 (MQ=255)                   caattTGATTGATAATCGTCCCGAATCATTATTAAATGCAATTCACAATAAGTATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGAttg                                                                                                                      <  1:243165/141‑3 (MQ=255)                    aattTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGt                                                                                                                     >  1:287663/1‑141 (MQ=255)                           ttgatAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTg                                                                                                              <  1:494421/141‑1 (MQ=255)                           ttgatAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTg                                                                                                              <  1:715191/141‑1 (MQ=255)                             tatAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGat                                                                                                            <  2:433789/140‑1 (MQ=255)                              atAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTGAAACAGTGTTAATGTGCTGGACAATAAATTTAAAGATGGTCTTTTTGagt                                                                                                           >  1:70998/1‑139 (MQ=255)                              atAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGatt                                                                                                           >  1:41461/1‑141 (MQ=255)                               tAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGatta                                                                                                          >  1:122687/1‑141 (MQ=255)                                  tCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGCGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATtc                                                                                                       >  1:34104/1‑141 (MQ=255)                                   cGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATtct                                                                                                      >  2:113578/1‑141 (MQ=255)                                   cGTCCAGAATCATTATTAGATGCAAATCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATtct                                                                                                      <  2:384663/141‑1 (MQ=255)                                    ggccAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATtctc                                                                                                     <  1:819346/139‑1 (MQ=255)                                    gTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGATATTTTTGATTATtctc                                                                                                     >  1:323554/1‑141 (MQ=255)                                     tCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTCAAGTATGGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCa                                                                                                    >  1:257887/1‑141 (MQ=255)                                       cAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGGTCGACAATAAATTTACAGATGGTATTTTTGATTATTCTCatg                                                                                                  >  1:527900/1‑141 (MQ=255)                                       cAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTTTTCTCatt                                                                                                  >  2:474542/1‑140 (MQ=255)                                          aaTCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCatgatg                                                                                               >  1:832201/1‑141 (MQ=255)                                             aattattAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAAt                                                                                            <  2:748391/140‑1 (MQ=255)                                                  ttaGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGGCCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAGTTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGt                                                                                       >  2:753025/1‑141 (MQ=255)                                                  ttaGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGt                                                                                       >  1:190841/1‑141 (MQ=255)                                                   taGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTg                                                                                      >  2:805996/1‑141 (MQ=255)                                                   taGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTg                                                                                      >  2:52294/1‑141 (MQ=255)                                                       tGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAAc                                                                                  <  1:324322/141‑1 (MQ=255)                                                        gCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACa                                                                                 <  1:226920/141‑1 (MQ=255)                                                        gCAAGTCAAAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGTACGACAAGAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACa                                                                                 <  1:304231/141‑1 (MQ=255)                                                          aaTTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAAt                                                                               <  2:503797/141‑1 (MQ=255)                                                           aTTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATa                                                                              <  1:548583/141‑1 (MQ=255)                                                            ttCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGAAAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAg                                                                             <  1:533263/141‑1 (MQ=255)                                                             tCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGc                                                                            <  1:43586/141‑1 (MQ=255)                                                              cacaACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTGACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACATTAGcc                                                                           >  1:610591/1‑141 (MQ=255)                                                               acaacaATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCct                                                                          >  2:616283/1‑141 (MQ=255)                                                                caacaaTGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCctc                                                                         >  1:426962/1‑141 (MQ=255)                                                                caacaaTGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCctc                                                                         >  1:16344/1‑141 (MQ=255)                                                                caacaaTGATGTTAAAGAATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCGAACAGTGTTAATGCGCTCGCCAAGAAATTTAAAGAGGGTATTGTTGATTATTCTCATGATGAATTGGGAGAAACAATAGCctc                                                                         >  2:3484/1‑141 (MQ=255)                                                                    aaTGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTAtt                                                                     <  1:330330/141‑1 (MQ=255)                                                                      tgatgTTAAAGTATTGGCAAGAGGACCGGGTTCCAAAGGATTATTAACTTCAATCAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTaa                                                                   <  2:212608/141‑1 (MQ=255)                                                                       gatgTTAAAGTATTGGCAATAGGACCTGTGTCTAAAGGATTATTAACTTCGAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGACGAATTGGGTGAAACAATAGCCTCTATTaaa                                                                  >  2:670042/1‑141 (MQ=255)                                                                        atgTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAg                                                                 >  1:834866/1‑141 (MQ=255)                                                                              aaGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTg                                                           >  1:87812/1‑141 (MQ=255)                                                                                   ttGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGt                                                      <  2:361925/141‑1 (MQ=255)                                                                                       cAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAAtt                                                  >  1:258691/1‑141 (MQ=255)                                                                                           agGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATc                                              <  1:473062/141‑1 (MQ=255)                                                                                           agGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATc                                              <  2:571536/141‑1 (MQ=255)                                                                                              aCCTGAGTCTAAAGGATTATTAACTTACAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTga                                           >  2:302718/1‑140 (MQ=255)                                                                                               ccTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGCAATTGAAAGTAATTTATCTGCa                                          >  1:117229/1‑141 (MQ=255)                                                                                                   tgtCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGa                                      >  1:433213/1‑141 (MQ=255)                                                                                                   tgtCTAAAGGATTATTAACTTCAAACAGTGTTAATGCGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTACCTACAGTGa                                      >  2:813817/1‑141 (MQ=255)                                                                                                    gtCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTTATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGAc                                     >  2:230641/1‑141 (MQ=255)                                                                                                    gtCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGAc                                     >  2:337255/1‑141 (MQ=255)                                                                                                    gtCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGAc                                     <  2:121753/141‑1 (MQ=255)                                                                                                             attattAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTa                            <  1:259228/141‑1 (MQ=255)                                                                                                                  taACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTTACATTTAGGTATTTaa                       >  1:39651/1‑141 (MQ=255)                                                                                                                   aaCTTCAAGCAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAGTTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAAc                      >  2:108091/1‑141 (MQ=255)                                                                                                                   aaCTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAAc                      >  1:55687/1‑141 (MQ=255)                                                                                                                    aCTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTTTTTTTTTTTATGATGAATTGGGGGAAACAATAGCATCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATATAGTTATTAAACa                     >  2:459464/1‑141 (MQ=255)                                                                                                                    aCTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTAAAATTTAGTTATTTAACa                     >  2:167582/1‑141 (MQ=255)                                                                                                                    aCTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATGAAAGTAAATTAAATTAATTTATCTGCATTGACATTTAGTTATTTAACa                     >  2:189392/1‑141 (MQ=255)                                                                                                                        cAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATcac                 >  1:824175/1‑141 (MQ=255)                                                                                                                        cAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATcac                 >  1:753251/1‑141 (MQ=255)                                                                                                                          aaCAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACAt               >  1:745641/1‑141 (MQ=255)                                                                                                                          aaCAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACAt               >  1:837114/1‑141 (MQ=255)                                                                                                                                   aaTGTTCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCtt      <  1:385816/141‑1 (MQ=255)                                                                                                                                   aaTGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCtt      <  1:846529/141‑1 (MQ=255)                                                                                                                                   aaTGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCtt      >  1:193635/1‑141 (MQ=255)                                                                                                                                    atttgCTCGACAATAAATTTAAAGATGGTATTTTTGTTTATTCTGATGATTAATCGGGTGAAACAATAGCCTCTATGAAAGAAATTTAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTg     <  2:80357/138‑1 (MQ=255)                                                                                                                                     tgtgTTCGACAAGAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTTGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTgg    <  2:515035/141‑1 (MQ=255)                                                                                                                                       tgCTTGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAGTTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTGGtt  <  2:103863/141‑1 (MQ=255)                                                                                                                                       tgCTTGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAGTTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTGGtt  <  2:342209/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         AACGATAATGTCTCAATTCAATTTGATTGATAATCGTCCAGAATCATTATTAGATGCAATTCACAACAATGATGTTAAAGTATTGGCAAGAGGACCTGTGTCTAAAGGATTATTAACTTCAAACAGTGTTAATGTGCTCGACAATAAATTTAAAGATGGTATTTTTGATTATTCTCATGATGAATTGGGTGAAACAATAGCCTCTATTAAAGAAATTGAAAGTAATTTATCTGCATTGACATTTAGTTATTTAACATCACATGACGTGCTTGGTT  >  CP000730/1616994‑1617268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑