Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,797 G→A 43.4% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,7970GA43.4% 69.2 / 128.3 121intergenic (‑880/‑288)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/36);  new base A (22/30);  total (55/66)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTC  >  CP000730/1929660‑1929931
                                                                                                                                         |                                                                                                                                           
 tCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCAga                                                                                                                                         >  2:688181/1‑141 (MQ=255)
   ataataAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGTGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatg                                                                                                                                       <  2:829607/141‑1 (MQ=255)
          aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataat                                                                                                                                >  1:208213/1‑141 (MQ=255)
            tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                              >  2:582428/1‑141 (MQ=255)
            tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                              >  1:371496/1‑141 (MQ=255)
            tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTAACTGTGCTTGTATATTTGCCGATGATATAAaag                                                                                                                              >  1:371350/1‑141 (MQ=255)
              gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAGGCAAGTGACTGTGCTTGTATATTTGCCGATGAATTAAtagtg                                                                                                                            >  2:301731/1‑140 (MQ=255)
              gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                            >  1:820416/1‑141 (MQ=255)
              gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                            >  2:504010/1‑141 (MQ=255)
               ccTAGTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTGTCAGAAGCAAGTGACTGTGCCTGTATATTTGCCGATAAGAGAAtagtag                                                                                                                           >  2:551202/1‑141 (MQ=25)
                  aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                        <  1:270173/141‑1 (MQ=255)
                  aCTTTCTTAAACTAACAATATCTATTCTCATATAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTAAGCTTAGATATTTGCCGATGATATAATAGTAGAGt                                                                                                                        <  2:377837/141‑1 (MQ=37)
                      tCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGcc                                                                                                                    <  2:255910/141‑1 (MQ=255)
                       cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                   >  1:714853/1‑141 (MQ=255)
                       cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                   >  1:714883/1‑141 (MQ=255)
                       cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAACTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCAACTGATATAATAGTAGAGTCGCCt                                                                                                                   <  2:457781/141‑1 (MQ=255)
                           aaTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTAGCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtct                                                                                                               >  1:555796/1‑141 (MQ=255)
                               aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTCGAGTCGCCTATCTCTCa                                                                                                           <  2:603149/141‑1 (MQ=255)
                                  aaTATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGc                                                                                                        >  2:753867/1‑141 (MQ=255)
                                    tatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                      <  1:219668/141‑1 (MQ=255)
                                          ttCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                <  1:322815/141‑1 (MQ=255)
                                          ttCCCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                <  2:258342/141‑1 (MQ=255)
                                               aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACg                                                                                           <  1:726022/141‑1 (MQ=255)
                                                tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGATTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGc                                                                                          >  2:239109/1‑141 (MQ=255)
                                                  gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                        <  1:57996/141‑1 (MQ=255)
                                                  gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                        <  1:502200/141‑1 (MQ=255)
                                                  gAATTTGTCCAATTAAGTGTAGACGATTCAATCGATCAGGTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGGGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                        <  2:435133/141‑1 (MQ=255)
                                                   actttGTGCAATTACGCGTAGACGCGCCAATCCATCAGCTAGAATCATATAACTTACCCGAAACACGTGCCTGTGCTAGTCTATACCCCGATCATATAATAGTAGAGTCGCATACTGCTCCAGCGTCAGTTCAGCCGCaga                                                                                       >  2:34265/3‑141 (MQ=255)
                                                   aaattGTCCAATTAAGTGTAGACGATTAAATCTATCAGCTTGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGGCAATTTAGACGCaga                                                                                       <  2:785526/138‑1 (MQ=255)
                                                    aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                      <  1:794918/141‑1 (MQ=255)
                                                      ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                    >  2:16187/1‑141 (MQ=255)
                                                          ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGgggagg                                                                                >  2:220951/1‑141 (MQ=255)
                                                          ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGaggagg                                                                                >  1:536532/1‑141 (MQ=255)
                                                          ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACGCCTATCTCTCGGGCGTCAATGTAGACGCcgagagacgg                                                                                >  1:233739/1‑137 (MQ=255)
                                                               tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGtat                                                                         >  1:332680/1‑141 (MQ=255)
                                                                  gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                     <  1:233854/141‑1 (MQ=255)
                                                                           aTTCAAGCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                          <  1:19522/141‑1 (MQ=255)
                                                                             tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                        <  2:820416/141‑1 (MQ=255)
                                                                             tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                        <  2:857778/141‑1 (MQ=255)
                                                                             tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                        <  1:504010/141‑1 (MQ=255)
                                                                             tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                        <  1:301731/141‑1 (MQ=255)
                                                                              cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtt                                                       >  1:828033/1‑141 (MQ=255)
                                                                                aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGTGGTGTATAAGGTGATGCTTAtttt                                                     <  1:582428/141‑1 (MQ=255)
                                                                                aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGGTGCGTAtttt                                                     >  1:324975/1‑141 (MQ=255)
                                                                                   tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGt                                                  >  1:140705/1‑141 (MQ=255)
                                                                                            gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCActtcat                                         <  2:555796/141‑1 (MQ=255)
                                                                                             aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTGTATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGGTCACATCATa                                        >  1:196502/1‑141 (MQ=255)
                                                                                                   ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCa                                  >  1:815784/1‑141 (MQ=255)
                                                                                                     aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGt                                <  2:283593/141‑1 (MQ=255)
                                                                                                           tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                          >  2:459763/1‑141 (MQ=255)
                                                                                                              atcggaattagatatgCATGTACATTCACAGATGCTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                      <  1:458780/127‑1 (MQ=255)
                                                                                                              atcggaagtatatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTTCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                      <  1:435092/127‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATATAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                      <  1:115700/127‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                      <  1:242005/127‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                      <  2:616381/127‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCATATCATAGCACCAGTCATCAGTGGc                      >  2:568844/15‑141 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGCTCAAGACATAGCACCAGTCATCAGCGGc                      >  2:560697/15‑141 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                     >  2:567492/14‑141 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                     >  1:112339/14‑141 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                     >  1:259999/14‑141 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCGTTACTTTCCTCATCGCTTTCGTTCACATCATAGCACCAGTCATCAGCGGCt                     >  1:616651/14‑141 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATCGTATAATAATAGAGTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                     >  1:653607/14‑141 (MQ=255)
                                                                                                               tcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCt                     >  2:199155/14‑141 (MQ=255)
                                                                                                                gggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                    <  2:436160/129‑1 (MQ=255)
                                                                                                                aggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGACGCCTATCTCTCAGGCGTCAA‑‑TGGACGAAGGGAGGAGGTGCATTACTCTGCTAAGCATCTCCGTTCACCTCATAGCCCCAGTGATCAGTGCCtg                    >  2:154804/13‑141 (MQ=255)
                                                                                                                 ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                   <  1:606532/130‑1 (MQ=255)
                                                                                                                 ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                   <  2:336666/130‑1 (MQ=255)
                                                                                                                   aagtagatatgCTTGTTTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGc                 <  1:356595/132‑1 (MQ=255)
                                                                                                                   aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                 <  2:638240/132‑1 (MQ=255)
                                                                                                                   aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                  >  1:189934/1‑141 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGGCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATGATAGCACCAGTCATCATTGGCTGTGctg               >  1:515352/8‑139 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTATGctg               >  1:725748/8‑139 (MQ=255)
                                                                                                                       gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATATTACGAGGCATCAGTGGCTGTGCCAt              <  2:646762/141‑1 (MQ=255)
                                                                                                                       gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt              <  1:691294/141‑1 (MQ=255)
                                                                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt             >  1:559027/6‑137 (MQ=255)
                                                                                                                        gatatgCTTGTATTTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg            >  1:572585/5‑136 (MQ=255)
                                                                                                                        gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg            >  1:556839/5‑136 (MQ=255)
                                                                                                                        gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg            <  1:882442/137‑6 (MQ=255)
                                                                                                                          tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCCATTGc           <  2:779727/141‑1 (MQ=255)
                                                                                                                          tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGCCATTGc           >  1:122779/1‑141 (MQ=255)
                                                                                                                          tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCg          >  1:208487/3‑141 (MQ=255)
                                                                                                                          tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACAAGTCATCATTGGCTGTGCTGTTGCg          >  2:226741/3‑141 (MQ=255)
                                                                                                                           atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt         <  1:637536/140‑1 (MQ=255)
                                                                                                                           atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt         <  1:633230/140‑1 (MQ=255)
                                                                                                                            tgCTTGTATATTCAAAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGGATTACTTTGCGAATCATTTTAGTTCACATCATAGCACCAGTCATCAGTTGCTGtgcttttgcttt        >  2:378795/1‑141 (MQ=255)
                                                                                                                              cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt      <  1:110514/141‑4 (MQ=255)
                                                                                                                              cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt      <  1:383506/141‑4 (MQ=255)
                                                                                                                              cTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTTTGAGGCGTCAAGTT‑‑ACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCTGTCATCAATGGCTGTGCTGTTGCGtatt      >  1:447839/1‑141 (MQ=255)
                                                                                                                               ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtccga     <  2:8910/141‑5 (MQ=255)
                                                                                                                               ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta     >  1:821819/1‑140 (MQ=255)
                                                                                                                               ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta     <  1:251866/141‑5 (MQ=255)
                                                                                                                               ttGTATATTCACAGAAGGTATAATATGAGAGTCGCCTAGCTCTCAGGCTTCAAGTT‑‑ACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGGCAGCAGTGGCTGTGCTGTTGCGtagta     <  2:166106/141‑5 (MQ=255)
                                                                                                                                tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGttttat    <  2:631480/141‑3 (MQ=255)
                                                                                                                                tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat    <  2:138253/141‑6 (MQ=255)
                                                                                                                                  tataTTTGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt   <  2:718397/141‑1 (MQ=255)
                                                                                                                                  tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac  <  2:307152/141‑5 (MQ=255)
                                                                                                                                  tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac  <  1:194973/141‑8 (MQ=255)
                                                                                                                                  tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac  <  2:429289/141‑8 (MQ=255)
                                                                                                                                   atatTTGCCGATGATATAATAGTAGAGTGGCATATCTCTCAGGCGTCAAGTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCCATTGCGTTTTTTTc  >  1:264965/1‑141 (MQ=255)
                                                                                                                                   atagtagCCTATTATATAATAGTGGAGTCGCCGATGTCTCAGGCGTCAATTTAGACGCGGAGAAGAGGTGTATAAGGTGATGCTTGTTTAAGTTCACCAAAAACAGCCAGTCATCCGTGGTTGTGCGATTGCGATttcttc  >  2:639315/7‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTC  >  CP000730/1929660‑1929931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: