BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,804	A→G	42.9%	intergenic (‑887/‑281)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,804	0	A	G	42.9%	29.2 / 141.0	120	intergenic (‑887/‑281)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (33/35); new base G (22/29); total (55/65)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-01

Only 100 of 133 total aligned reads displayed.

AATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAA  >  CP000730/1929670‑1929942

aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataat                                                                                                                                           >  1:208213/1‑141 (MQ=255)

  tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                                         >  1:371496/1‑141 (MQ=255)

  tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTAACTGTGCTTGTATATTTGCCGATGATATAAaag                                                                                                                                         >  1:371350/1‑141 (MQ=255)

    gCCTACTTTCTTAAATTAACAATATCTATTCTCCTTACACTTGTCTGGTTGACTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                       <  1:295617/141‑1 (MQ=38)

    gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAGGCAAGTGACTGTGCTTGTATATTTGCCGATGAATTAAtagtg                                                                                                                                       >  2:301731/1‑140 (MQ=255)

    gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                       <  2:461480/141‑1 (MQ=255)

    gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                       >  1:820416/1‑141 (MQ=255)

     ccTAGTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTGTCAGAAGCAAGTGACTGTGCCTGTATATTTGCCGATAAGAGAAtagtag                                                                                                                                      >  2:551202/1‑141 (MQ=25)

        aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                                   <  1:270173/141‑1 (MQ=255)

        aCTTTCTTAAACTAACAATATCTATTCTCATATAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTAAGCTTAGATATTTGCCGATGATATAATAGTAGAGt                                                                                                                                   <  2:377837/141‑1 (MQ=37)

            tCTTAAATTAACAATATCTATTCTCATAGAATGTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGcc                                                                                                                               <  2:386968/141‑1 (MQ=255)

             cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                              >  1:714883/1‑141 (MQ=255)

             cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAACTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCAACTGATATAATAGTAGAGTCGCCt                                                                                                                              <  2:457781/141‑1 (MQ=255)

                     aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTCGAGTCGCCTATCTCTCa                                                                                                                      <  2:603149/141‑1 (MQ=255)

                        aaTATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGc                                                                                                                   >  2:753867/1‑141 (MQ=255)

                          tatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                                 <  1:219668/141‑1 (MQ=255)

                                ttCCCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                           <  2:258342/141‑1 (MQ=255)

                                      tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGATTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGc                                                                                                     >  2:239109/1‑141 (MQ=255)

                                        gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                   <  1:57996/141‑1 (MQ=255)

                                        gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                   <  1:502200/141‑1 (MQ=255)

                                        gAATTTGTCCAATTAAGTGTAGACGATTCAATCGATCAGGTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGGGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                   <  2:435133/141‑1 (MQ=255)

                                         actttGTGCAATTACGCGTAGACGCGCCAATCCATCAGCTAGAATCATATAACTTACCCGAAACACGTGCCTGTGCTAGTCTATACCCCGATCATATAATAGTAGAGTCGCATACTGCTCCAGCGTCAGTTCAGCCGCaga                                                                                                  >  2:34265/3‑141 (MQ=255)

                                         aaattGTCCAATTAAGTGTAGACGATTAAATCTATCAGCTTGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGGCAATTTAGACGCaga                                                                                                  <  2:785526/138‑1 (MQ=255)

                                          aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                                 <  1:794918/141‑1 (MQ=255)

                                              gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgagga                                                                                             >  2:180522/1‑141 (MQ=255)

                                                ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGaggagg                                                                                           >  1:536532/1‑141 (MQ=255)

                                                ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACGCCTATCTCTCGGGCGTCAATGTAGACGCcgagagacgg                                                                                           >  1:233739/1‑137 (MQ=255)

                                                     tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑ta‑t                                                                                    >  1:332680/1‑141 (MQ=255)

                                                        gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑g                                                                                <  1:233854/141‑1 (MQ=255)

                                                         tgtAGACGATTCAAGCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCTCTCCGGCGTCAATTTAGACGCAGAGGGGAGGTG‑TA‑TAA‑g‑g                                                                              >  1:504572/1‑141 (MQ=255)

                                                               cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGc                                                                       <  1:79055/141‑1 (MQ=255)

                                                                   tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTAt                                                                   <  1:301731/141‑1 (MQ=255)

                                                                   tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTAt                                                                   <  2:820416/141‑1 (MQ=255)

                                                                    cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTAtt                                                                  >  1:828033/1‑141 (MQ=255)

                                                                      aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGTGGTG‑TA‑TAA‑G‑G‑TGATGCTTAtttt                                                                <  1:582428/141‑1 (MQ=255)

                                                                      aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGGTGCGTAtttt                                                                >  1:324975/1‑141 (MQ=255)

                                                                                  gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCActtcat                                                    <  2:555796/141‑1 (MQ=255)

                                                                                  gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCAcatcat                                                    <  1:16187/141‑1 (MQ=255)

                                                                                   aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTGTATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGGTCACATCATa                                                   >  1:196502/1‑141 (MQ=255)

                                                                                           aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGt                                           <  2:283593/141‑1 (MQ=255)

                                                                                                 tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                     >  2:459763/1‑141 (MQ=255)

                                                                                                    atcggaattagatatgCATGTACATTCACAGATGCTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                 <  1:458780/127‑1 (MQ=255)

                                                                                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATATAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                 <  1:115700/127‑1 (MQ=255)

                                                                                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                 <  2:616381/127‑1 (MQ=255)

                                                                                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                 >  2:618088/15‑141 (MQ=255)

                                                                                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                 <  1:242005/127‑1 (MQ=255)

                                                                                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCATATCATAGCACCAGTCATCAGTGGc                                 >  2:568844/15‑141 (MQ=255)

                                                                                                     tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                >  1:259999/14‑141 (MQ=255)

                                                                                                     tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                >  1:112339/14‑141 (MQ=255)

                                                                                                     tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                >  2:567492/14‑141 (MQ=255)

                                                                                                     tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCGT‑TAC‑T‑T‑TCCTCATCGCTTTCGTTCACATCATAGCACCAGTCATCAGCGGCt                                >  1:616651/14‑141 (MQ=255)

                                                                                                     tcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCt                                >  2:199155/14‑141 (MQ=255)

                                                                                                      aggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGACGCCTATCTCTCAGGCGTCAA‑‑TGGACGAAGGGAGGAGGTG‑CATTAC‑T‑C‑TGCTAAGCATCTCCGTTCACCTCATAGCCCCAGTGATCAGTGCCtg                               >  2:154804/13‑141 (MQ=255)

                                                                                                      aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                >  1:559205/1‑141 (MQ=255)

                                                                                                       ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                              <  1:343615/130‑1 (MQ=255)

                                                                                                       ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                              <  1:606532/130‑1 (MQ=255)

                                                                                                         aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                            <  2:638240/132‑1 (MQ=255)

                                                                                                         aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                             >  1:189934/1‑141 (MQ=255)

                                                                                                           gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGGCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATGATAGCACCAGTCATCATTGGCTGTGctg                          >  1:515352/8‑139 (MQ=255)

                                                                                                           gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTATGctg                          >  1:725748/8‑139 (MQ=255)

                                                                                                             gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATATTACGAGGCATCAGTGGCTGTGCCAt                         <  2:646762/141‑1 (MQ=255)

                                                                                                             gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                         <  1:691294/141‑1 (MQ=255)

                                                                                                             agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                        >  1:559027/6‑137 (MQ=255)

                                                                                                             agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                        <  2:245269/136‑5 (MQ=255)

                                                                                                              gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                       <  1:882442/137‑6 (MQ=255)

                                                                                                              gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                       >  1:556839/5‑136 (MQ=255)

                                                                                                                tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGCCATTGc                      >  1:122779/1‑141 (MQ=255)

                                                                                                                tatgCTTGTATATTCACATATTGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                     <  2:318809/139‑1 (MQ=255)

                                                                                                                tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                     >  1:208309/3‑141 (MQ=255)

                                                                                                                tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACAAGTCATCATTGGCTGTGCTGTTGCg                     >  2:226741/3‑141 (MQ=255)

                                                                                                                gatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTGTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTCAGCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                     <  2:56404/139‑1 (MQ=255)

                                                                                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                    <  1:637536/140‑1 (MQ=255)

                                                                                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                    >  2:810351/2‑141 (MQ=255)

                                                                                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                    <  1:633230/140‑1 (MQ=255)

                                                                                                                    cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                 <  1:110514/141‑4 (MQ=255)

                                                                                                                    cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                 <  1:383506/141‑4 (MQ=255)

                                                                                                                    cTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTTTGAGGCGTCAAGTT‑‑ACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCTGTCATCAATGGCTGTGCTGTTGCGtatt                 >  1:447839/1‑141 (MQ=255)

                                                                                                                    cTTGAATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                 <  1:698023/141‑4 (MQ=255)

                                                                                                                     ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtccga                <  2:8910/141‑5 (MQ=255)

                                                                                                                     ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                >  1:821819/1‑140 (MQ=255)

                                                                                                                     ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                <  1:251866/141‑5 (MQ=255)

                                                                                                                     ttGTATATTCACAGAAGGTATAATATGAGAGTCGCCTAGCTCTCAGGCTTCAAGTT‑‑ACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGGCAGCAGTGGCTGTGCTGTTGCGtagta                <  2:166106/141‑5 (MQ=255)

                                                                                                                      tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat               <  2:138253/141‑6 (MQ=255)

                                                                                                                        tataTTTGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt              <  2:718397/141‑1 (MQ=255)

                                                                                                                        tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac             <  2:429289/141‑8 (MQ=255)

                                                                                                                        tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac             <  2:307152/141‑5 (MQ=255)

                                                                                                                        tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac             <  1:194973/141‑8 (MQ=255)

                                                                                                                         atattgacagatggtATAATAATAGAGTCGCCTAGCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact            <  2:26993/132‑6 (MQ=255)

                                                                                                                         atagtagCCTATTATATAATAGTGGAGTCGCCGATGTCTCAGGCGTCAATTTAGACGCGGAGAAGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTGTTTAAGTTCACCAAAAACAGCCAGTCATCCGTGGTTGTGCGATTGCGATttcttc             >  2:639315/7‑141 (MQ=255)

                                                                                                                            ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCAATCACATCATAGCACCAGTCATCACTGTCAGTGCTGTTGCGTATTATACTTAt         >  2:145154/7‑141 (MQ=255)

                                                                                                                              cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg       >  1:95288/5‑141 (MQ=255)

                                                                                                                              cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATAAGTGGCTGGGCTGTTGCGTATTATACTTATTg       >  2:91759/5‑141 (MQ=255)

                                                                                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc     <  2:104301/139‑1 (MQ=255)

                                                                                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc     <  1:766080/139‑1 (MQ=255)

                                                                                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc     <  2:914863/139‑1 (MQ=255)

                                                                                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc     <  2:337430/139‑1 (MQ=255)

                                                                                                                                 cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc     <  1:723492/141‑1 (MQ=255)

                                                                                                                                 agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCCATT‑‑GACGAAGGGAGGAGGTG‑CA‑TTT‑CTT‑TGCTAGTCATTTTCGTTGACATCGTAGGACCGGTCGTCGGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt    <  2:667089/140‑1 (MQ=255)

                                                                                                                                  gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt   <  2:559027/141‑2 (MQ=255)

                                                                                                                                    tgatATAATAGTAGAGTCGCCTATCTCTCAGGAGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa  >  1:653723/1‑141 (MQ=255)

AATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAA  >  CP000730/1929670‑1929942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`