Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,274,436 A→G 5.0% T454T (ACA→ACG mtlF → PTS family fructose/mannitol (fru) porter component IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,274,4360AG 5.0% 218.7 / 3.2 80T454T (ACA→ACGmtlFPTS family fructose/mannitol (fru) porter component IIBC
Reads supporting (aligned to +/- strand):  ref base A (43/32);  new base G (2/2);  total (46/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01

GAAGATGTTGATGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA  >  CP000730/2274296‑2274574
                                                                                                                                            |                                                                                                                                           
gAAGATGTTGATGCACACAATTACAATAATATAAATCATGTTCTTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACa                                                                                                                                             <  2:833410/141‑1 (MQ=255)
     tGTTGATGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATaa                                                                                                                                       >  2:79529/1‑140 (MQ=255)
     tGTTGATGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATAc                                                                                                                                       >  1:771171/1‑141 (MQ=255)
           tGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGAt                                                                                                                                 <  1:696784/141‑1 (MQ=255)
            gCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGAtt                                                                                                                                <  2:207326/141‑1 (MQ=255)
                acaAATACAATCATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTGCTAGTGCAAGGAGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGTGCATGAATGATATGACAGTGACAACTGCAGCGAGTAAta                                                                                                                            >  2:555988/1‑140 (MQ=255)
                 caATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCa                                                                                                                           >  1:288198/1‑141 (MQ=255)
                 caATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGAGGGTATGGGTTAAAAAGCAATGGGTGCACGAATCTCCAACGCGAGGGTTAGAAAAACAGACATTAATGATATTACAGTTACAAATACTGCGATTAATCa                                                                                                                           >  2:877515/1‑141 (MQ=255)
                  gaTTATAATCATATAAATCATGTTATTATTAACTTCGATGTGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTGCAGTTACAAATACTGCGATTAATCaa                                                                                                                          >  1:669605/2‑141 (MQ=255)
                     tACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGAAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATAATGCGATTAATCAATTg                                                                                                                       >  2:97843/1‑141 (MQ=255)
                      aCAATAATATAAATCATGTTATTTTTTTTTTTTTTTCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGTGCATTAATGATATTACACACACAAATACTACGATTAATATATTGc                                                                                                                      >  2:711106/1‑141 (MQ=255)
                      aCAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTTCAGTTACAAATACTGCGATTAATCAATTGc                                                                                                                      >  1:67643/1‑141 (MQ=255)
                      aCAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGc                                                                                                                      <  2:65673/141‑1 (MQ=255)
                       aaataacAAAAATCATGTTACTTTGGCGTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAACGATATTACAGTTACAAATACTGCGATTAATCAATTGcc                                                                                                                     <  1:93351/140‑1 (MQ=255)
                        aataatATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCa                                                                                                                    <  1:212644/141‑1 (MQ=255)
                          taataTAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCGTGTTACGTACTAAATTTAAAAAGGCGGACATTAAGGATATTACATTTACAAATGCTGCGATTCATTAATTGCCaaa                                                                                                                  >  2:284929/1‑141 (MQ=255)
                             tataAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGa                                                                                                               >  1:490337/1‑141 (MQ=255)
                              ataATTCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGAt                                                                                                              <  1:419207/141‑1 (MQ=255)
                                 aaTCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGAGGCt                                                                                                           >  1:414152/1‑141 (MQ=255)
                                   tCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCGAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCa                                                                                                         <  2:72923/141‑1 (MQ=255)
                                   tCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAGTACTGCGATTAATCAATTGCCAAAAGATGCTCa                                                                                                         >  2:215946/1‑141 (MQ=255)
                                    cATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCaa                                                                                                        <  1:306422/141‑1 (MQ=255)
                                     aTGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTCCAAATAATGCGATTAATCAATTGCCAAAAGATGCTCAAt                                                                                                       >  2:804868/1‑141 (MQ=255)
                                         tATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGt                                                                                                   >  1:109692/1‑141 (MQ=255)
                                         tATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGt                                                                                                   >  1:908536/1‑141 (MQ=255)
                                         tATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGt                                                                                                   >  1:653721/1‑141 (MQ=255)
                                         tATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGt                                                                                                   >  1:702298/1‑141 (MQ=255)
                                           tttttGCCTGCGATGCGGGTATGGGTACTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATGTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCACAATTAGTTa                                                                                                 >  2:545147/1‑141 (MQ=255)
                                               tGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCAc                                                                                             >  1:31474/1‑141 (MQ=255)
                                               tGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCAc                                                                                             >  2:44931/1‑141 (MQ=255)
                                                gCCTGCGATGCGGGTATGGGTTATAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGACTAATCAATTGCCAAAAGATGCCCACTTAGTCATCAcc                                                                                            >  2:128342/1‑140 (MQ=255)
                                                 ccTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAACAGATGCTCAACTAGCTAACACta                                                                                           >  2:680775/1‑140 (MQ=255)
                                                   tGCGAGGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGAATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAg                                                                                         <  2:314657/141‑1 (MQ=255)
                                                      gATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGaaa                                                                                      >  1:763257/1‑141 (MQ=255)
                                                           gggTTTGGTTTCTAGTGCAATGGGTGCACGAATGTTACGTAATAAATTGAAAAAAGCGGGCATTAATGATAATCCAGTTATAAATACTGCGATTAATCAATTTCCAAAAGATGCTAAATTAGTTAACAAACAGAAAAaact                                                                                 <  2:85662/141‑1 (MQ=255)
                                                           gggTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAaact                                                                                 >  1:18732/1‑141 (MQ=255)
                                                            ggTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACGAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAaacta                                                                                >  1:790236/1‑141 (MQ=255)
                                                            ggTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACGAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAaacta                                                                                >  1:772644/1‑141 (MQ=255)
                                                             gTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGGTATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCGATTAGTTATGACTCAGATAAacctaa                                                                               >  2:91355/1‑141 (MQ=255)
                                                               aTGGGTTCTAGTGCGATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAaactaact                                                                             <  2:868035/141‑1 (MQ=255)
                                                                  ggTTCTAGTGCAATGGATGCAAACATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATACTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGAAAAAAAAAAAAAAAAACTAACTGAt                                                                          <  2:788997/141‑1 (MQ=255)
                                                                      ttAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTg                                                                      <  2:713050/140‑1 (MQ=255)
                                                                      cTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTg                                                                      >  2:795538/1‑141 (MQ=255)
                                                                       tAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGc                                                                     <  1:911679/141‑1 (MQ=255)
                                                                         gcgCAATGGGTGCAAGCATGTTACGTAACAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTa                                                                   <  1:274977/139‑1 (MQ=255)
                                                                                 ggTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACGAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTaaacaa                                                           <  2:772644/141‑1 (MQ=255)
                                                                                 ggTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACGAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTAGTaaacaa                                                           <  2:790236/141‑1 (MQ=255)
                                                                                  gtacAATCATGTTACGTAATAAACTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTaaacaaa                                                          <  2:503007/138‑1 (MQ=255)
                                                                                  gggaaaGTATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATTATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAATTAGCTTATCGTGCTATTaaacaaa                                                          <  2:195784/137‑1 (MQ=255)
                                                                                    gCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAGTTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAaaaaaaca                                                        >  2:679045/1‑141 (MQ=255)
                                                                                     cAAGCATGTTACGTAATAAATTTAAAAAGGCGGGGATTAAGGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAacac                                                       <  2:705679/141‑1 (MQ=255)
                                                                                      aaGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACAcc                                                      <  1:724286/141‑1 (MQ=255)
                                                                                        acATGTTACGTAATAAATTTAAAAAGTCTGGCATTAATGATATCACAGTTACAAATACTGCGATTAACCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACGAACTGACCCTGCTACTAAACAAACACCaa                                                    <  2:8099/140‑1 (MQ=255)
                                                                                         cATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCaaa                                                   >  1:801687/1‑141 (MQ=255)
                                                                                         cATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCaaa                                                   <  2:18732/141‑1 (MQ=255)
                                                                                          aTGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAAt                                                  <  1:847985/141‑1 (MQ=255)
                                                                                                cGTAATAAAATTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGccatc                                            >  1:667757/1‑141 (MQ=255)
                                                                                                 gTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGccatcc                                           <  2:771171/141‑1 (MQ=255)
                                                                                                   aataaATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGccatccat                                         <  2:442934/141‑1 (MQ=255)
                                                                                                    ataaATTTAAAAAGGCGGTCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCata                                        <  2:141618/141‑1 (MQ=255)
                                                                                                            aaaaaGGCGGGCATTAATGATATTACAGTTCCCACTTACATCATGAACCAATAGACAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCGATTAAACAAACGCCAAATGCCATCCATCTTTCAGTg                                >  1:436396/1‑141 (MQ=255)
                                                                                                            aaaaaGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTCTTAAACAAACACCAAATGCCATCCATATTTCAGTg                                >  1:73870/1‑141 (MQ=255)
                                                                                                             aaaaGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTgg                               >  1:882769/1‑141 (MQ=255)
                                                                                                             aaaaGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTgg                               >  1:278529/1‑141 (MQ=255)
                                                                                                             aaaaGGCGGGCAGTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTgg                               <  2:156582/141‑1 (MQ=255)
                                                                                                                   cGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAAtt                         <  1:369607/141‑1 (MQ=255)
                                                                                                                   cGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAAtt                         >  2:629434/1‑141 (MQ=255)
                                                                                                                   cGGACACTAATGATAGTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGATATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAAtt                         <  2:218149/141‑1 (MQ=255)
                                                                                                                     ggCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTc                       >  2:455964/1‑141 (MQ=255)
                                                                                                                      gCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTcc                      >  1:499325/1‑141 (MQ=255)
                                                                                                                       cATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCt                     <  1:514323/141‑1 (MQ=255)
                                                                                                                        aTTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATACCACCCATATTTCAGTGGATAATTTCCtt                    >  2:426657/1‑141 (MQ=255)
                                                                                                                          tAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTaa                  >  2:699334/1‑141 (MQ=255)
                                                                                                                           aaTGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAACCACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAAt                 <  1:480184/141‑1 (MQ=255)
                                                                                                                           aaTGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAAt                 <  1:877515/141‑1 (MQ=255)
                                                                                                                           aaTGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAAt                 >  1:53101/1‑141 (MQ=255)
                                                                                                                            aTGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAAtt                >  1:635546/1‑141 (MQ=255)
                                                                                                                               atatTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAAttaca             >  1:377640/1‑138 (MQ=255)
                                                                                                                               atatTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCAc             >  1:491049/1‑141 (MQ=255)
                                                                                                                                      aGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGata      >  1:760856/1‑141 (MQ=255)
                                                                                                                                          aCAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATgaa  <  1:490460/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GAAGATGTTGATGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAACAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA  >  CP000730/2274296‑2274574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: