Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6980CA91.4% 306.8 / 24.7 105coding (1216/1260 nt)USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (42/52);  minor base . (7/2);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 8.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*CP0007302,455,6990AT98.0% 296.2 / ‑5.4 105H406L (CAT→CTT) USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (48/51);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 105 total aligned reads displayed.

AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  CP000730/2455568‑2455825
                                                                                                                                  ||                                                                                                                                
aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaaaa                                                                                                                       >  1:326947/1‑141 (MQ=255)
      aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGCGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaa                                                                                                                >  1:319968/1‑141 (MQ=255)
       cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaag                                                                                                               <  1:51484/141‑1 (MQ=255)
       cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaag                                                                                                               <  1:704739/141‑1 (MQ=255)
       cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaag                                                                                                               >  1:613118/1‑141 (MQ=255)
       cAAGAAGCATAAAGTGACGATATAACAAAAAATACAAATGAAAATGAAATCGAGCAATCAGTAAAAGAACCTGCGACTTACAAAGACGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTgaag                                                                                                               <  2:632848/141‑1 (MQ=255)
        aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaaga                                                                                                              <  2:504721/141‑1 (MQ=255)
        aaGCACCACAAAGTGACGATTAAAAAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAATATAGAAAAAACTgaaga                                                                                                              <  2:10007/141‑1 (MQ=255)
          gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAAc                                                                                                            >  1:713806/1‑141 (MQ=255)
          gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAAc                                                                                                            <  1:843822/141‑1 (MQ=255)
           caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAGAAATTATCGAAAAAACTGAAGAACa                                                                                                           >  2:604766/1‑141 (MQ=255)
           caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCACTAAAAGCACCTGCGACGTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACa                                                                                                           >  1:180670/1‑141 (MQ=255)
               acaAAGTGACGATTTAACAAACTATTCAAATGAAGATAAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                       <  1:176841/141‑1 (MQ=255)
               acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTTCAGAAAAAACTGAAGGACAATCt                                                                                                       >  1:18077/1‑141 (MQ=255)
                  aagcaacaaTTTAACAAACGATTCAAATTAAGATGAAATAGAGCAATCAGTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCa                                                                                                    <  2:86924/133‑1 (MQ=255)
                  aaGTGACGATTAAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCa                                                                                                    <  2:159905/141‑1 (MQ=255)
                   attGACGAGCTAACAAACGGTGCAGATGAAAATGAAATTGAGCAATGATTAAAAGAACCTGCGACTTATAAAGAATACAGACTTTCACCAGTTTTAATTGATGCAAAAAAATGATCGAAAAAACTGAAGAACAATCTTCAg                                                                                                   <  2:25743/139‑1 (MQ=255)
                   aGTGACGAATTAACAAAAGATTAAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGAAAAAAAAAAAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg                                                                                                   <  2:462890/141‑1 (MQ=255)
                    gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                  <  1:489446/141‑1 (MQ=255)
                     tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                 <  1:346334/141‑1 (MQ=255)
                      caCGATTAAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATa                                                                                                <  1:890175/140‑1 (MQ=255)
                        cGATTTAACAAACGATTGAAGTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                              <  2:825574/141‑1 (MQ=255)
                             tAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTAAGATAAAAACaa                                                                                         >  2:437739/1‑141 (MQ=255)
                                  aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaataat                                                                                    >  2:708698/1‑141 (MQ=255)
                                   aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata                                                                                   <  2:669186/141‑1 (MQ=255)
                                     gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACATATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc                                                                                 <  2:897316/141‑1 (MQ=255)
                                      aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa                                                                                <  1:332517/141‑1 (MQ=255)
                                       ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                               <  1:501733/141‑1 (MQ=255)
                                       atgaaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                               <  2:785216/138‑1 (MQ=255)
                                          aaaaGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc                                                                            <  1:723532/141‑1 (MQ=255)
                                          aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATAAGTTGTTATTGATGCAAAACATCTTCGAAAAATACTGAAGACAAATCTTGCGGGTAAAACAAATAATAAAAAAgc                                                                            >  1:658115/1‑141 (MQ=255)
                                           aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAgcg                                                                           >  2:394998/1‑141 (MQ=255)
                                           aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTAGAAAGAATACAGACGTTCATCATTTGTAATTGATGCAAAAAAATTATCGAAAAAACGGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                           <  2:444748/141‑1 (MQ=255)
                                                aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGc                                                                      <  2:13796/141‑1 (MQ=255)
                                                  aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTa                                                                    <  1:421932/141‑1 (MQ=255)
                                                   tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                   >  1:279364/1‑141 (MQ=255)
                                                    gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                  >  1:525681/1‑141 (MQ=255)
                                                      aaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGCTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACCATCTCCAGATAAGAACAAATAATAACAAAGCGCTAGCTAGCAc                                                                >  1:111734/1‑141 (MQ=255)
                                                       aTAGAGCAATCATTCAAAGCACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                               >  1:499868/1‑141 (MQ=255)
                                                       aTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                               >  1:423226/1‑141 (MQ=255)
                                                         agagCAAACATTAAAAGAACCTACGAGTTATACAGAAGACAGACGTTCATGAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAGCAAAGCGCTTGCTAGTACctc                                                             <  2:865797/141‑1 (MQ=255)
                                                          agggaaacATTAAAAAAACATACGACTTATAAAGAAGACAGACGTTCATTAGTTGTAATTGATGCAAAAAAATAATAGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                            <  2:6960/134‑1 (MQ=255)
                                                           agCAATCATTAAAAGAACCTGCGACTTGTAAACAAAACAGACGTGCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTAAGATAAAAAAAAATAAAAACAAAGCGCTTGCTAGTACCTCtt                                                           >  2:52352/1‑141 (MQ=255)
                                                           agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt                                                           >  2:782480/1‑141 (MQ=255)
                                                           agCAATCATTAAAAGAACCGGCGACTTATAAAGAGGACAGACGTTCATTAGTTGTAATTGAGGCGAAGAAATAAGCGAAAAAAGTGAAGAGCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCGAGTACCTCtt                                                           >  1:680993/1‑141 (MQ=255)
                                                             cAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAGCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa                                                         >  2:277828/1‑141 (MQ=255)
                                                              aaacATTAAAAGAACCTGCGACTTATAAAGAAAACAGCCATTAATACATAGTCATTGGTACGATAAAACTATCGAAAAAATCGAAGTACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  2:191623/138‑1 (MQ=255)
                                                              aaacATTAAAAGAACCTGCGACTTACAAAGAAGACAGACGTACATCAGTTGTAATAGATGCAAAAAAATTATCGAAAAAACTGAACAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  1:254408/138‑1 (MQ=255)
                                                              aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  2:302616/141‑1 (MQ=255)
                                                              aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  1:513771/141‑1 (MQ=255)
                                                               aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                       <  1:166715/141‑1 (MQ=255)
                                                               aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAGTAATGGAACAAAATTATCGAAAAAACTGAAGAACAATCTGCAGATAAAAACAAATAATAACAAAGCGCTTGATATGACCTCTTaaga                                                       >  1:253965/1‑139 (MQ=255)
                                                                tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa                                                      >  1:817113/1‑141 (MQ=255)
                                                                 cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                     <  2:14945/141‑1 (MQ=255)
                                                                  aTTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAga                                                    >  2:168243/1‑141 (MQ=255)
                                                                   ttAAGAGAACCTGCGACTTATAAAGAAGACCGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGATTGCTAGTAACTCTTAAAAAgac                                                   >  2:554784/1‑140 (MQ=255)
                                                                   ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAGCAAACTTCAGATAAAAACGAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat                                                   <  2:818051/141‑1 (MQ=255)
                                                                     aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCGAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTACAAAgatga                                                 >  1:395517/1‑141 (MQ=255)
                                                                       aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                               <  2:180670/141‑1 (MQ=255)
                                                                         gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                             <  1:379274/141‑1 (MQ=255)
                                                                          aaCCTACAACATATAAAGAAGACAGACGTTCATCAGTAGAAAAAAAAAAAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTa                                            <  2:609547/141‑1 (MQ=255)
                                                                           aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAGGCTGCTAg                                           >  2:859343/1‑141 (MQ=255)
                                                                            ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTGGc                                          >  2:749491/1‑141 (MQ=255)
                                                                            ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc                                          >  2:742796/1‑141 (MQ=255)
                                                                              tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGcca                                        >  1:398279/1‑139 (MQ=255)
                                                                              tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                        <  2:644744/141‑1 (MQ=255)
                                                                               gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                       <  2:768309/141‑1 (MQ=255)
                                                                                cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:845056/1‑141 (MQ=255)
                                                                                cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:648315/1‑141 (MQ=255)
                                                                                cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      >  1:712516/1‑141 (MQ=255)
                                                                                cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                      <  1:273517/141‑1 (MQ=255)
                                                                                  aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGCTATTAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    <  1:394998/141‑1 (MQ=255)
                                                                                  aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    >  2:701018/1‑141 (MQ=255)
                                                                                  aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                    >  2:815026/1‑141 (MQ=255)
                                                                                  aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTACCAAgcgc                                    >  1:73446/1‑141 (MQ=255)
                                                                                   cTTCTAAAGAAGACAGACGTTCAGCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                   <  1:764243/141‑1 (MQ=255)
                                                                                      ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                <  1:741196/141‑1 (MQ=255)
                                                                                      ataAAGAAGACAGACGTTCATCAGTTGTAATAGATACAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAGCGCtttt                                <  2:221500/141‑1 (MQ=255)
                                                                                       taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               >  2:168651/1‑141 (MQ=255)
                                                                                       taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               >  1:455535/1‑141 (MQ=255)
                                                                                       aaaaGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAACAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc                               <  2:722937/140‑1 (MQ=255)
                                                                                          agaagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCTAGCGCGTTTctat                            >  1:286962/1‑141 (MQ=255)
                                                                                           gaagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctata                           >  2:803081/1‑141 (MQ=255)
                                                                                            aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTATCAGGGGCttttttctat                          >  2:78996/1‑135 (MQ=255)
                                                                                            aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTCAAAAGATGATGCTAGCAAGCGCGTTTctatac                          >  2:629861/1‑141 (MQ=255)
                                                                                                 agagGTTCATCACTTGTAATTCATGCAAAAAAATTATCAAAAAAACTTAATAACAATCTTCAGATAAAAACAAATAACAACAAAGCGCTTGCTAGTACCTCTTAAATAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  2:387410/141‑1 (MQ=255)
                                                                                                 agacGTTTATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACAGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  1:2522/141‑1 (MQ=255)
                                                                                                 agacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     <  2:515001/141‑1 (MQ=255)
                                                                                                 agacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                     >  2:212396/1‑141 (MQ=255)
                                                                                                  gacGTTCATCAGTTGTGAGTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAGCGCTTTTCTATACtatata                    <  2:365630/141‑1 (MQ=255)
                                                                                                  gacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAGCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACcatata                    >  2:467448/1‑141 (MQ=255)
                                                                                                  gacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTCTATACtatata                    >  2:520137/1‑141 (MQ=255)
                                                                                                    cGTTCATCAGTTGTAATTGATGCAAAAAAATTGTCGAAAAAACTGACGAACAGTCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTGCCGGTGAAAAAGCAGATGCTAGCAGCCCCTCTTgtatacaatggatt                  >  2:475553/1‑136 (MQ=255)
                                                                                                    cGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTAAAtatt                  >  2:161574/1‑141 (MQ=255)
                                                                                                       tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt               <  2:606687/141‑1 (MQ=255)
                                                                                                         atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt             <  1:708698/141‑1 (MQ=255)
                                                                                                           caGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc           <  1:834976/141‑1 (MQ=255)
                                                                                                            aGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCCCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTCTATATTATTTTTCt          >  2:516928/1‑141 (MQ=255)
                                                                                                                gTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaaa      <  2:720161/141‑1 (MQ=255)
                                                                                                                    ttGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt  <  1:152395/141‑1 (MQ=255)
                                                                                                                                  ||                                                                                                                                
AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  CP000730/2455568‑2455825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: