Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT99.2% 380.6 / NA 119T15T (ACA→ACTmurNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (31/87);  minor base C (0/1);  total (31/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACT  >  CP000730/2532156‑2532441
                                                                                                                                              |                                                                                                                                               
ttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                           <  2:904520/149‑1 (MQ=255)
      gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                                     >  2:863023/1‑149 (MQ=255)
        gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  2:241909/149‑1 (MQ=255)
        gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  2:277323/149‑1 (MQ=255)
        gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGAGGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                           <  1:801950/141‑1 (MQ=255)
        gtATTTGTAAACAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGACCTTTGCAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                   <  2:314166/149‑1 (MQ=255)
         tATTTGTAATCAGTTTTTGATAAAAAAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                          <  1:936472/141‑1 (MQ=255)
          aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                                 <  2:556069/149‑1 (MQ=255)
           tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAt                                                                                                                                        <  1:119635/141‑1 (MQ=255)
                  tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGATTACTACACAGCATTTTTCACATTacaca                                                                                                                         <  2:483414/149‑1 (MQ=255)
                     gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                                      <  2:373487/149‑1 (MQ=255)
                         ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                  <  2:156795/149‑1 (MQ=255)
                         ttGATAAAATAAAAAAATAGCACTATTAATAAATTCAAAAATTATAGTACAATTCAGAGAGGGATGGGATAAAATGAACCTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                  <  2:79473/149‑1 (MQ=255)
                          tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAGTGCGTTTATTACAAAGAATTTTTCACATTataaa                                                                                                                         >  1:563294/1‑137 (MQ=255)
                           gaaaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                                <  2:382054/146‑1 (MQ=255)
                           gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                        >  1:343136/1‑141 (MQ=255)
                           gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                                <  2:382010/149‑1 (MQ=255)
                           aataaaataaaAAAACAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                        <  1:773001/140‑1 (MQ=255)
                            ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                                       >  1:1041167/1‑141 (MQ=255)
                             taaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                                      >  1:415574/1‑141 (MQ=255)
                              aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGTGATAACATGAACGTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                                     <  1:933725/141‑1 (MQ=255)
                                  aaaaaaaaGAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAtt                                                                                                         <  2:433138/148‑1 (MQ=255)
                                    aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACAATCacata                                                                                                               >  1:375675/1‑137 (MQ=255)
                                     aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa                                                                                                      <  2:343256/149‑1 (MQ=255)
                                      aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                                     <  2:720781/149‑1 (MQ=255)
                                       aaaTAGCATTATTAATAAATTCAAACATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGATTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  2:541736/149‑1 (MQ=255)
                                       aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTGTTCATTACAAt                                                                                                    >  2:911595/1‑149 (MQ=255)
                                       aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  2:429347/149‑1 (MQ=255)
                                       aaaTAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  2:230896/149‑1 (MQ=255)
                                       aaaTAGCATTATTAACAACTTCAACAATTAGACTTCAATTCCGAGAGCGATGTGATAAAATGAACTTTGTAACGTTGACCTCGGATCCGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  2:541778/149‑1 (MQ=255)
                                        aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                           >  1:1063939/1‑141 (MQ=255)
                                        aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAAAATCATACACAATCAGCTATTCa                                                                                                           <  1:849012/141‑1 (MQ=255)
                                          aaGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAt                                                                                                 <  2:526790/148‑1 (MQ=255)
                                               ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCTGCTCTTCATTACAAt                                                                                                    <  1:1182603/141‑1 (MQ=255)
                                               ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:412917/141‑1 (MQ=255)
                                               ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAACATAGAGt                                                                                            >  2:347550/1‑149 (MQ=255)
                                               tgagtaataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                    <  1:137604/137‑1 (MQ=255)
                                                tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                           <  2:664548/149‑1 (MQ=255)
                                                 attaATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCa                                                                                                  <  1:616631/141‑1 (MQ=255)
                                                   taataaATTCAAAAATTATAGGTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                                <  1:409158/141‑1 (MQ=255)
                                                    aataaATTCAAAAATTATAGTTCCATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAtt                                                                                       <  2:458243/149‑1 (MQ=255)
                                                     ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGCAAAGGTAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTATTTACAAACATAGAGTTGAttt                                                                                      >  2:451459/1‑149 (MQ=255)
                                                      aaaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                                     <  2:715741/148‑1 (MQ=255)
                                                      aaaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                                     <  2:777706/148‑1 (MQ=255)
                                                       aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAGTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                                    <  2:165036/149‑1 (MQ=255)
                                                         aTTCAAAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCGATGTATTACAATCATAGAGTTGATTTaaaa                                                                                  <  2:224796/149‑1 (MQ=255)
                                                              aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATCAACTTAGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTGTTCATTCCACTCATAGAGTTGCTTTa                                                                                     >  1:635771/1‑141 (MQ=255)
                                                                aaTTATAGTTCAATCCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGCTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa                                                                                   <  1:933677/141‑1 (MQ=255)
                                                                   tataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAgg                                                                                <  1:840236/141‑1 (MQ=255)
                                                                     taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                      <  2:836796/149‑1 (MQ=255)
                                                                     taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                      <  2:47022/149‑1 (MQ=255)
                                                                     aaGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                      <  2:297848/148‑1 (MQ=255)
                                                                         tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  >  2:125801/1‑149 (MQ=255)
                                                                         tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                                  >  2:852968/1‑149 (MQ=255)
                                                                          cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTACAAGGCGAtgtg                                                                         <  1:731811/141‑1 (MQ=255)
                                                                             ttCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATCGGAATTCGTGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                              <  2:223013/149‑1 (MQ=255)
                                                                             ttCAGAGAGGGATGGGATAAAATGAACTATGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGACTTAAAAGGCGATGTGCATCTTGTAgg                                                              >  2:565850/1‑149 (MQ=255)
                                                                             ctccgggagGGAGTGGAGATAATGAACTGTGTCACTTTGGCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCAAATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAACAGGCGATGTGCAt                                                                      <  1:476639/135‑1 (MQ=255)
                                                                               ccggtagGGATGGGATAAAAAGACCTTTGTAACGTTGTCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  2:577945/144‑1 (MQ=255)
                                                                               cAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                            <  2:316791/149‑1 (MQ=255)
                                                                                agagagGGATGGGATAAAATGAAGTTTGTAACGTTGACTTCGGATGAGTCCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                                   <  1:76865/141‑1 (MQ=255)
                                                                                agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTTCAAGCATAGAGTTGATTTGAAAGGCGATGTGCATGTTGTAGGGGt                                                           >  2:374430/1‑149 (MQ=255)
                                                                                agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTCGGGGt                                                           >  2:489899/1‑149 (MQ=255)
                                                                                 gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCATTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAATGAGTTGATTTAAAACGCGATGACCATCtgt                                                                  >  1:355471/1‑139 (MQ=255)
                                                                                   gaggcttgggatAAAATGAACTTTGCCTCACATACTCCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                        <  2:438849/143‑1 (MQ=255)
                                                                                   gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                        <  2:20053/149‑1 (MQ=255)
                                                                                   gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATCAGTTCAATGCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                        <  2:268786/149‑1 (MQ=255)
                                                                                    agGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaaa                                                       <  2:875564/149‑1 (MQ=255)
                                                                                        attggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACCACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                           <  1:707996/138‑1 (MQ=255)
                                                                                        atgggatAAAATGAAGTTTGTAACGTTGACTTCGTATGAGTTTATTTAGTTTACTACAAAGCTTTTTGCACATTAGACACAATCAGGTATTTATTACAATCAGAGAGTTGATTTAAAAGGCGATGTGCAGCTTGTAGGGGt                                                           <  1:240030/141‑1 (MQ=255)
                                                                                        atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  2:106401/149‑1 (MQ=255)
                                                                                        atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  2:711734/149‑1 (MQ=255)
                                                                                        atgggatAAAATGAACTTTGTAAAGTTGAATTCGGATGAGTTCAATGAGTTTAGTACAAAGCATTTTTGACATTACACACAATCAGCTATTAATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATATTGTAGGGGt                                                           >  1:1083889/1‑141 (MQ=255)
                                                                                         tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                          >  1:831418/1‑141 (MQ=255)
                                                                                         tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  2:522353/149‑1 (MQ=255)
                                                                                         tgggatAAAATGAACCTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                                  <  2:217616/149‑1 (MQ=255)
                                                                                          gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAc                                                 <  2:238820/149‑1 (MQ=255)
                                                                                          gggatAAAATGAACGTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAc                                                 <  2:474699/149‑1 (MQ=255)
                                                                                             atAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                              <  2:378000/149‑1 (MQ=255)
                                                                                              tAAAAGGAGCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGa                                                     <  1:338337/141‑1 (MQ=255)
                                                                                                aaaGGAACTTTGTAACGTTGCCTTCGGATGAGTTCAACGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCACTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                   <  1:355342/141‑1 (MQ=255)
                                                                                                  aTGGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                         <  2:331664/149‑1 (MQ=255)
                                                                                                      actttgatccatggACTTCGGATGACTTCAATGCGTTTACTACAAAGCATTTTTCAAATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                                             <  1:591745/128‑1 (MQ=255)
                                                                                                      aCTTTGTAACGTTGACTTCGGATCAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                                             >  1:853671/1‑141 (MQ=255)
                                                                                                       cTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg                                            <  1:501430/141‑1 (MQ=255)
                                                                                                          tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                         >  1:875437/1‑141 (MQ=255)
                                                                                                            tAACGTTGACTTCGGATGAGTTCAATGCGTTTGCTACAAAGCATTTTTCACATTACACACAATCTGCTATTCATTACAATCATAGAGTTGTTTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAGAATGGTCAAg                                       >  1:284615/1‑141 (MQ=255)
                                                                                                                gTTGACTTCGGATGAGTTCAATGCGTTCACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                                   <  1:745493/141‑1 (MQ=255)
                                                                                                                   ggcTTCGGATGGGTGCAATGCGTTTACTACAAAGCATTTTTTACATTAAACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAGGACAATGGTCAAGTGATTGc                                <  1:1090274/139‑1 (MQ=255)
                                                                                                                    aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                               >  1:1105369/1‑141 (MQ=255)
                                                                                                                        cGGATGAGTTCAATGCGGTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                           <  1:590122/141‑1 (MQ=255)
                                                                                                                         ggATGAGTTCAATGCGTTTACTACAAAGCATGTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                  <  2:352229/149‑1 (MQ=255)
                                                                                                                               gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAACGATGACAATGGTCAAGTGATTACAGGATGCTTAtt                    >  1:630462/1‑141 (MQ=255)
                                                                                                                                 tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                  >  1:915433/1‑141 (MQ=255)
                                                                                                                                    aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg               <  1:948973/141‑1 (MQ=255)
                                                                                                                                    aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgc       <  2:799676/149‑1 (MQ=255)
                                                                                                                                    aTGCGTTGACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAGCATAGAGTTGATTTAAAAGGCTATGTGCATCTTGTATGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg               <  1:163470/141‑1 (MQ=255)
                                                                                                                                        gTTTACTTCAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  2:166820/149‑1 (MQ=255)
                                                                                                                                        gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCacac   <  2:906734/149‑1 (MQ=255)
                                                                                                                                         tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACt  <  2:714368/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACT  >  CP000730/2532156‑2532441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: