Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 59.9% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC59.9% 143.0 / 290.6 310intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (78/45);  new base C (94/89);  total (173/137)
Fisher's exact test for biased strand distribution p-value = 4.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 315 total aligned reads displayed.

TATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAA  >  CP000730/76155‑76429
                                                                                                                                            |                                                                                                                                      
tataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                                                                                                                                        <  1:341334/141‑1 (MQ=38)
   aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                                                                                                                                     <  1:307768/141‑1 (MQ=2)
    ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCGGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                                                                                                                                    >  1:323099/1‑141 (MQ=25)
     gTTCTGTTGCAAAGTTGAATATATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCg                                                                                                                                   >  1:218156/1‑141 (MQ=12)
               aaaGTTGAATCGATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATAAAAACAATTTAACATGGATGTTATCACTGTAGCTGTTGGCTACTATCTAGGTTATGCTTTTAGTTATCGTGatattttt                                                                                                                         >  1:158931/1‑137 (MQ=11)
                 aGTGGAATATATAGTATACTTTTAACAAAAAGGAAACTTCAAAAAGAAATCTATCAGTTATGAACAAATTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                                                       <  2:343648/141‑1 (MQ=11)
                  gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa                                                                                                                      <  1:16055/141‑1 (MQ=35)
                      cagttataGTATACTTTTAACAAAAACGAGTCTTCTGTATGAACTATTTCCGATATAACCAATTTAACAAGGATGTTATCACTGTCGCTGTTGGCTACTCTCTAAGATATGCATTGAGTTATCGTGATATATATGCAatat                                                                                                                  >  2:159164/5‑141 (MQ=11)
                       atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                 >  2:247797/4‑141 (MQ=0)
                       atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                 >  2:213473/4‑141 (MQ=0)
                       aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATCTATCTGAAATAtt                                                                                                                 >  1:6792/1‑141 (MQ=25)
                       aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt                                                                                                                 >  1:130160/1‑141 (MQ=34)
                        tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGCTATATCTGAAATATTa                                                                                                                >  2:355798/3‑141 (MQ=2)
                        tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                                                >  1:334938/3‑141 (MQ=2)
                           ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                                             >  1:153176/1‑141 (MQ=32)
                             aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                                           <  1:124471/139‑1 (MQ=12)
                               tataCTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAc                                                                                                         >  2:248313/1‑141 (MQ=12)
                                ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACg                                                                                                        >  1:157151/1‑141 (MQ=17)
                                  aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                      >  1:333132/1‑141 (MQ=17)
                                     tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCAGAAATATTAAAGGAACgtggtg                                                                                                   >  1:62251/1‑141 (MQ=17)
                                      ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                                                  <  1:356396/141‑1 (MQ=12)
                                        aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCTTGATATAGCTGAAATATAAAGGGAACGTGGTGTaa                                                                                                >  2:276531/1‑141 (MQ=11)
                                        aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                >  1:110039/1‑141 (MQ=17)
                                        aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                <  2:188673/141‑1 (MQ=17)
                                        aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                >  1:257763/1‑141 (MQ=12)
                                        aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCGAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                >  2:62109/1‑141 (MQ=12)
                                         aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaga                                                                                               >  1:269845/1‑139 (MQ=12)
                                          cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATCAAGGGAACGTGGGGTaaca                                                                                              >  2:139895/1‑139 (MQ=255)
                                             aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                                           <  1:171109/141‑1 (MQ=12)
                                              aagtattcctctgtctgAACTATTTCCGATATAAACAATTTAACCAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                          <  1:4629/132‑1 (MQ=11)
                                              aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                          >  1:273584/1‑141 (MQ=17)
                                              aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                          <  1:95371/141‑1 (MQ=12)
                                                 gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGGGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                       >  1:207723/1‑141 (MQ=12)
                                                         tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACg                                                                               >  1:318873/1‑141 (MQ=17)
                                                          gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                              <  1:150627/141‑2 (MQ=12)
                                                             tGAATTTTTTGAGATATAAACAATTTAACAAGGATGTTAGCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAACGTGATATACCTGAAATATTAAGGGCACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                           <  2:11705/141‑1 (MQ=11)
                                                                  tagctCAGAAATAGACAAGTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAACGTGATATATCTGAAATAGTAAGGGAACGTGGTGTAATCGTTCATCATTCAACGGTCTACCGt                                                                      <  1:134638/137‑1 (MQ=11)
                                                                  tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                      <  1:52169/141‑1 (MQ=12)
                                                                  tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                      <  2:70224/141‑1 (MQ=255)
                                                                  tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                      <  1:345572/141‑1 (MQ=255)
                                                                   aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                     <  1:40864/141‑1 (MQ=12)
                                                                    tttCAGATATAAAAAATTTAACAAGGATGTTAACACTGTAGCCGTTGGCTACTATCTAAGAAATGCATTGAGATATCGTAATATATCTGAAATATTAAGAGAACGTGTTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                    <  2:147920/141‑1 (MQ=11)
                                                                      tCAGGTATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                  >  1:244583/1‑141 (MQ=17)
                                                                      tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                  >  1:77351/1‑141 (MQ=17)
                                                                      tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                  <  2:130160/141‑1 (MQ=32)
                                                                      tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                  >  1:106265/1‑141 (MQ=255)
                                                                        aGATATACAACATTTAACAAGGATGTTATCACTGTAGCCGTTAGCGACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                >  2:121147/1‑141 (MQ=11)
                                                                        aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTACACGTTCATCATTCAACGATCTACCGTGGGGtt                                                                >  2:315976/1‑141 (MQ=11)
                                                                        aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                >  1:281471/1‑141 (MQ=12)
                                                                             taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                           <  1:248313/141‑1 (MQ=17)
                                                                             aaaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                           <  2:184803/140‑1 (MQ=255)
                                                                              aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                                          >  1:18537/1‑141 (MQ=17)
                                                                                aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTGGGTTCAAGAata                                                        >  1:290760/1‑141 (MQ=17)
                                                                                 cAATTTAACAAGGATGTTATCACTGTAACCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                       >  1:191654/1‑141 (MQ=11)
                                                                                    tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                    <  2:143516/141‑1 (MQ=17)
                                                                                    tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                    <  2:204315/141‑1 (MQ=255)
                                                                                      tAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                  >  1:215348/1‑141 (MQ=17)
                                                                                       aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                 >  2:306337/1‑141 (MQ=32)
                                                                                        aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                >  1:101635/1‑141 (MQ=255)
                                                                                        aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                <  2:281471/141‑1 (MQ=255)
                                                                                        aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                >  1:203002/1‑141 (MQ=255)
                                                                                         cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                     <  2:102099/119‑1 (MQ=255)
                                                                                         cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                               <  2:290754/141‑1 (MQ=255)
                                                                                          aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                              >  2:114818/1‑141 (MQ=32)
                                                                                             gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                           <  1:299420/141‑1 (MQ=255)
                                                                                               tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGCAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTAGGTTCAAGAATATGGCCCAATTTtata                                         >  1:330457/1‑141 (MQ=255)
                                                                                               tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                         <  2:293937/141‑1 (MQ=255)
                                                                                                gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTGCAAGAATATGCCCCAATTTTatat                                        >  1:163872/1‑141 (MQ=255)
                                                                                                cttATCACTTTAGCTTTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATACCCGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                        <  1:116250/140‑1 (MQ=11)
                                                                                                 ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                       >  1:249917/1‑141 (MQ=255)
                                                                                                 ttATCACTGGAGCTGTTGGCTACTATCTAAGATATGCATTGATTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                       <  1:227537/141‑1 (MQ=255)
                                                                                                  tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                      <  1:54602/141‑1 (MQ=25)
                                                                                                    tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCAGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                    >  2:260928/1‑141 (MQ=255)
                                                                                                     cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                   >  2:192826/1‑141 (MQ=255)
                                                                                                      aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt                                  >  2:94364/1‑141 (MQ=25)
                                                                                                      aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATAAAAtt                                  >  1:296649/1‑141 (MQ=255)
                                                                                                         gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                               >  2:39994/1‑141 (MQ=255)
                                                                                                         gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                               <  1:233886/141‑1 (MQ=255)
                                                                                                          tAGGCGTTGGCTAGTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACTGTTGGGTTCAAGAATATGTCCCAATTTTATTTCAAATTTGga                              <  2:32155/141‑1 (MQ=11)
                                                                                                          tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaaaaaaaa                              <  2:273584/141‑7 (MQ=11)
                                                                                                          tAGCCGTTGGCTACTACCTAAGATATGCATTGAGGTCTCGTGATATATCTGAAATATGAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTGCAAGAATAGGCCCCAAGTTTATATCAAAtcagga                              >  2:332890/1‑136 (MQ=11)
                                                                                                             ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                           >  2:16003/1‑141 (MQ=255)
                                                                                                             ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgagaa                           >  2:58262/1‑138 (MQ=11)
                                                                                                              cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGCACGTGGTGTAAACCTTCATCATTCAACGATCTACCGTTGGATTAAAGAATATGCCCCAATTTTANATCAAATTTGcaataa                          >  1:276661/1‑141 (MQ=11)
                                                                                                                ttGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATACCATTTGgaaacaaa                        >  2:312649/1‑136 (MQ=11)
                                                                                                                   gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATGTCAAATTTGGAAGAAAAAGc                     <  1:84664/141‑1 (MQ=25)
                                                                                                                   gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                     <  2:219407/141‑1 (MQ=35)
                                                                                                                   gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAga                     >  2:48702/1‑140 (MQ=255)
                                                                                                                    acactaTCAAATAAATGGATTGAGGTATCGTGATATAACTGAAAAAAAAAAAAAAAATAGTGTAAACGTTCAACAGACAACGATCTACCGCTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGaaaaaaaaaaa                    <  2:80756/139‑4 (MQ=2)
                                                                                                                     tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                   >  1:246925/1‑141 (MQ=255)
                                                                                                                       ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                 <  2:352030/141‑1 (MQ=255)
                                                                                                                         aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa               >  1:36556/1‑141 (MQ=35)
                                                                                                                          tCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa              <  2:330841/141‑1 (MQ=35)
                                                                                                                              aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct          >  2:238296/1‑137 (MQ=11)
                                                                                                                                atatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAGGCATAAAAAGCTAAt        >  2:178061/1‑141 (MQ=255)
                                                                                                                                 tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat       >  1:91784/1‑134 (MQ=1)
                                                                                                                                 tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat       >  2:255304/1‑134 (MQ=11)
                                                                                                                                 tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat       <  1:287958/141‑8 (MQ=1)
                                                                                                                                   tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTaa     >  1:181012/1‑140 (MQ=40)
                                                                                                                                      aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaaa  >  2:165513/1‑141 (MQ=39)
                                                                                                                                            |                                                                                                                                      
TATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAA  >  CP000730/76155‑76429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: