Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 59.3% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA59.3% 111.9 / 297.4 280intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (65/49);  new base A (81/84);  total (146/134)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 286 total aligned reads displayed.

ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAA  >  CP000730/76182‑76461
                                                                                                                                            |                                                                                                                                             
  aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                                                                             >  1:130735/3‑141 (MQ=12)
  aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                                                             <  2:215482/141‑1 (MQ=17)
    tataCTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAc                                                                                                                                           >  2:248313/1‑141 (MQ=12)
    tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAc                                                                                                                                           >  1:332509/1‑141 (MQ=17)
     ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                                                          >  1:48312/1‑141 (MQ=17)
      taATTTTAACAACAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                                                         >  1:186133/1‑141 (MQ=17)
      taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                                                         <  1:307232/141‑1 (MQ=12)
      taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTAAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTCATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                                                         >  1:240126/1‑141 (MQ=12)
         ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAGCATTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGGGCTTTCGTGATATATCTGAAATATTAAGAGAAggtggt                                                                                                                                      >  2:195417/1‑141 (MQ=11)
         ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTAAGTTATCGTGATATATCTGAAATATTAAGGGAAAgtggt                                                                                                                                      >  1:145649/1‑141 (MQ=12)
            tAACAAAAATGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                                                   <  2:191154/141‑1 (MQ=17)
               cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGCACGTGGTGTAAAc                                                                                                                                >  2:93655/1‑141 (MQ=12)
               cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATCAAGGGAACGTGGGGTaaca                                                                                                                                >  2:139895/1‑139 (MQ=255)
                  aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGtt                                                                                                                             >  1:117007/1‑141 (MQ=17)
                   aaGGAGTCTTCTGTCTGATCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtc                                                                                                                            <  1:256156/141‑1 (MQ=12)
                   aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAGGTGGTGTAAACGTtc                                                                                                                            >  2:210294/1‑141 (MQ=17)
                   aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                                                            <  1:95371/141‑1 (MQ=12)
                      gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                                                         <  1:343227/141‑1 (MQ=17)
                       aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAAGGTtcatat                                                                                                                        >  2:109875/1‑139 (MQ=255)
                        gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                                                                                       <  1:61663/141‑1 (MQ=17)
                        gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATCAAGGGAACGTGGTGTAAACGTTcatcat                                                                                                                       >  1:68517/1‑141 (MQ=17)
                         tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATAGACTAAACGTGATATATCTGAAATAGTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                                                      >  1:259263/1‑141 (MQ=11)
                           ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCa                                                                                                                    <  1:52921/141‑1 (MQ=17)
                            tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa                                                                                                                   >  1:299251/1‑141 (MQ=32)
                               gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                                <  1:273409/141‑2 (MQ=12)
                                tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                                                                               >  2:355688/1‑139 (MQ=12)
                                 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                              <  1:20452/141‑1 (MQ=17)
                                  tGAATTTTTTGAGATATAAACAATTTAACAAGGATGTTAGCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAACGTGATATACCTGAAATATTAAGGGCACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                                                             <  2:11705/141‑1 (MQ=11)
                                    accTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                           <  2:283895/139‑1 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                           >  1:13745/1‑141 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAGGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                           >  1:163188/1‑141 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                           >  2:323810/1‑141 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTAc                                                                                                           >  2:33898/1‑141 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAACTGAAAAATTAAGGGAACGTGGTGTAAACGTTAATCATTCAACGGTCTAc                                                                                                           >  2:94879/1‑141 (MQ=12)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                           >  2:291938/1‑141 (MQ=12)
                                        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                       <  2:46129/141‑1 (MQ=12)
                                         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAATGGGTGAAAACGTTCATCATTCAACGGTCTACCGTTg                                                                                                      >  1:8753/1‑141 (MQ=11)
                                         tttCAGATATAAAAAATTTAACAAGGATGTTAACACTGTAGCCGTTGGCTACTATCTAAGAAATGCATTGAGATATCGTAATATATCTGAAATATTAAGAGAACGTGTTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                      <  2:147920/141‑1 (MQ=11)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                    >  2:57752/1‑141 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                    >  1:77351/1‑141 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                    <  2:130160/141‑1 (MQ=32)
                                             aGATATACAACATTTAACAAGGATGTTATCACTGTAGCCGTTAGCGACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                  >  2:121147/1‑141 (MQ=11)
                                             aGATATAAACACTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                  >  1:114609/1‑141 (MQ=11)
                                             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTACACGTTCATCATTCAACGATCTACCGTGGGGtt                                                                                                  >  2:315976/1‑141 (MQ=11)
                                             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTACACGTTCATCATTAAACGATGTATCGTTGGGtt                                                                                                  >  1:230578/1‑141 (MQ=11)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCaa                                                                                               <  1:349564/141‑1 (MQ=255)
                                                  taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                             <  1:248313/141‑1 (MQ=17)
                                                    aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                           >  1:320538/1‑141 (MQ=12)
                                                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                         <  1:151245/141‑1 (MQ=255)
                                                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                         >  1:36470/1‑141 (MQ=12)
                                                       aaTTTAACAAAGATGTTATCACTGTAGCCGTGTGCTACCATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                        <  2:7415/141‑1 (MQ=11)
                                                        aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                                                                                       >  2:178143/1‑141 (MQ=12)
                                                         tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                      <  2:143516/141‑1 (MQ=17)
                                                          ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAGTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                     <  1:107113/141‑1 (MQ=17)
                                                          ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                     >  2:349399/1‑141 (MQ=255)
                                                           tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                    >  2:238936/1‑141 (MQ=255)
                                                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                  >  1:101635/1‑141 (MQ=255)
                                                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                  >  2:18726/1‑141 (MQ=255)
                                                              cAAGTATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                 <  1:21114/141‑1 (MQ=255)
                                                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCACCATTGAACGATCTACCGTTGGGATCAGGAATATACCCCAAtt                                                                                >  2:236308/1‑141 (MQ=11)
                                                                 ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                              >  2:158449/1‑141 (MQ=255)
                                                                   aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                            <  2:332509/141‑1 (MQ=255)
                                                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                           <  2:293937/141‑1 (MQ=255)
                                                                     gTTATTACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                          <  1:281402/141‑1 (MQ=255)
                                                                      ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                         >  1:249917/1‑141 (MQ=255)
                                                                      ttATCACTGGAGCTGTTGGCTACTATCTAAGATATGCATTGATTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                         <  1:227537/141‑1 (MQ=255)
                                                                       tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGACTATGCCCCAATTTTATATCa                                                                        >  2:276819/1‑141 (MQ=21)
                                                                       tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                        >  2:47773/1‑141 (MQ=255)
                                                                        aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                       >  1:39099/1‑141 (MQ=255)
                                                                         tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCAGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                      >  2:260928/1‑141 (MQ=255)
                                                                          cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                                                     >  2:275393/1‑141 (MQ=255)
                                                                           aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATAAAAtt                                                                    >  1:296649/1‑141 (MQ=255)
                                                                              gTAGCTGTTTGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                 <  2:61837/141‑1 (MQ=11)
                                                                               tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaaaaaaaa                                                                <  2:273584/141‑7 (MQ=11)
                                                                                 gCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                              >  2:169725/1‑141 (MQ=35)
                                                                                  ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                             >  2:16003/1‑141 (MQ=255)
                                                                                   tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGGGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATATGgaagaa                                                            >  1:192582/2‑141 (MQ=21)
                                                                                   cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                            <  1:292017/141‑1 (MQ=255)
                                                                                     ttGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                                                          >  1:176302/1‑141 (MQ=35)
                                                                                      tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                         >  1:334859/1‑141 (MQ=35)
                                                                                       ggCTACTATCTAAGATATGCATTGAGTTATCGGTATAGATCGGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                        <  1:153709/141‑1 (MQ=255)
                                                                                        gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                       <  1:274760/141‑1 (MQ=34)
                                                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                     >  1:246925/1‑141 (MQ=255)
                                                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                     <  1:43012/141‑1 (MQ=255)
                                                                                            ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTTGGTTCAATAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                  <  2:312778/141‑1 (MQ=25)
                                                                                             taTCTAACATAGGAATTTAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCGATTTTATATCAAACTTCGAAGAAAAAGCATaaa                                                 <  1:158870/141‑1 (MQ=11)
                                                                                              aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                >  1:124652/1‑141 (MQ=35)
                                                                                                 tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                                             >  2:306752/1‑139 (MQ=21)
                                                                                                     atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                         >  1:103303/1‑135 (MQ=11)
                                                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                        >  1:91784/1‑134 (MQ=1)
                                                                                                      tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                        <  1:287958/141‑8 (MQ=1)
                                                                                                      tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                        >  2:255304/1‑134 (MQ=11)
                                                                                                        tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTaa                                     >  1:181012/1‑140 (MQ=40)
                                                                                                          cATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAAGCTAATTACa                                    >  2:123323/1‑141 (MQ=1)
                                                                                                           aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaaa                                  >  2:165513/1‑141 (MQ=39)
                                                                                                                   aTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATAGGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAt                          <  2:6792/141‑1 (MQ=255)
                                                                                                                      ggtaaatatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATACGCAGCCGGAGTACAAATGGCGTATtg                        <  1:355520/136‑1 (MQ=11)
                                                                                                                            tatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGAc                 >  1:262605/1‑141 (MQ=38)
                                                                                                                               ttGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAACTGGCGTATTGATGAGACGTa              <  1:216849/140‑1 (MQ=255)
                                                                                                                                 gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACa            <  1:169725/141‑1 (MQ=40)
                                                                                                                                            |                                                                                                                                             
ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAA  >  CP000730/76182‑76461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: