Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,850 C→T 46.8% intergenic (+81/+27) rlmH → / ← tpn hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8500CT46.8% 30.7 / 66.3 59intergenic (+81/+27)rlmH/tpnhypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (18/13);  new base T (15/12);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTA  >  CP000730/35711‑35963
                                                                                                                                           |                                                                                                                  
tcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAcc                                                                                                                   <  1:104874/141‑1 (MQ=255)
  aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                                 >  2:133359/1‑141 (MQ=255)
  aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                                 <  1:241880/141‑1 (MQ=255)
          aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                                         >  2:309625/1‑141 (MQ=255)
                 aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACAACTAATTTATCATGTCa                                                                                                  >  1:123909/1‑141 (MQ=255)
                        aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                           >  2:29741/1‑141 (MQ=255)
                        aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                           <  1:142358/141‑1 (MQ=255)
                         aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑c                                                                                         >  2:175547/1‑141 (MQ=255)
                          gCTATTCAGTATAAAGTTTTTGGCGAGAAATGATTACTTGAACAAGAGCTAAAATAAATATGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTG‑Ct                                                                                        >  2:177437/1‑141 (MQ=255)
                          gCTATTCAGTATAAAGTTTTTGGAGAAAAATGATTACTTGAACTAGAGCTAAAATTAAATTGTGATTTAATAAAACTAGAAGGTTATGTTGCATAGTAATAGAATATAGCTAAGCACTAATTTATCATGTCGGTGTTCgat                                                                                         >  2:268054/1‑139 (MQ=255)
                           cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑Ctt                                                                                       >  2:275276/1‑141 (MQ=255)
                           cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑Ctt                                                                                       <  1:289245/141‑1 (MQ=255)
                             atgcAGTAGAAAGTTTTTGGCGACAAATGATTACTTGAACAAGAGCTAAAATAAAATCGGGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATCTAGCTACCCAATAATTGAACAGGGCAGCATACG‑CTTaa                                                                                     >  2:193245/4‑141 (MQ=25)
                                      aaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGc                                                                            >  1:121688/1‑141 (MQ=255)
                                               ggCGAAAAATGATTACTTGAACAAGAGCTACAATAAAATTGTGATATCATCAAAATCGACGATACTGTCGCAAAGTAAAAAAATATAGTTAACCACTAATTAATCAGGTCACGGTTCG‑CTTAACTGGCTAGCATGATGATa                                                                   >  2:262252/1‑141 (MQ=18)
                                                     aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCg                                                             >  2:118006/1‑141 (MQ=255)
                                                          cttACTTGAACACGAGCTAAAATAAAATTGTGATCTACTAAAAATAGAAGGCTGTGTGGCGAGGTCAACACATATACATAGCCAATAATTTATAAGGTTAGTGTTCG‑CTTAACTTCGTTGCATGACGCTAATTTctgggcc                                                        >  2:154662/2‑135 (MQ=255)
                                                               ttGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                                   <  2:276320/141‑1 (MQ=255)
                                                                       gagCTAAAATAAACATGTGATCTAATAAAAAAAGAAGGTTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAAATTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                           >  2:12374/1‑141 (MQ=17)
                                                                          cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAg                                        >  2:356805/1‑141 (MQ=255)
                                                                           taaaataaaaTTGTGATCCAATAAAAATAGAAGGTTCTGTTGCAAAATAAAAAAATATAGCTAGCCACTAATTTATCATGTCAGTGTTAG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                       >  1:158792/1‑141 (MQ=255)
                                                                            aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGTGCTACCATG‑TACCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                                      >  1:116326/1‑141 (MQ=17)
                                                                            aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                                      <  2:121688/141‑1 (MQ=255)
                                                                                 aaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGTCGAAAATCCACACATCTGAaga                               >  2:142730/13‑141 (MQ=11)
                                                                                   aagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                             <  1:289486/131‑1 (MQ=11)
                                                                                   aagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                             >  1:43498/11‑141 (MQ=11)
                                                                                    agattattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAATCTATCGATACTATTTTATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  2:343690/132‑1 (MQ=2)
                                                                                    agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagaggc                            >  1:97609/10‑139 (MQ=11)
                                                                                    agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            >  1:68271/10‑141 (MQ=11)
                                                                                    agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  2:156940/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  2:191624/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTACTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            >  2:195761/10‑141 (MQ=11)
                                                                                     gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                           <  2:346702/133‑1 (MQ=11)
                                                                                     gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                           <  2:336269/133‑1 (MQ=11)
                                                                                      tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  1:246851/141‑1 (MQ=255)
                                                                                      tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  1:29741/141‑1 (MQ=255)
                                                                                      tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAAAATTGTTTCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                            <  1:352139/141‑1 (MQ=14)
                                                                                      agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                          <  2:42724/134‑1 (MQ=11)
                                                                                      agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGCCGAAAAGCCGTAGATCTGAAGAGACCt                          <  2:164678/134‑1 (MQ=11)
                                                                                       gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                         <  1:83195/135‑1 (MQ=11)
                                                                                       gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                         >  1:166320/7‑141 (MQ=11)
                                                                                        tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATGACTAATATATAATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAGAGCCGTAGATCTGAAGTGACATgg                        >  2:271487/6‑140 (MQ=11)
                                                                                        tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                        <  1:295848/136‑1 (MQ=11)
                                                                                         attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                       >  1:254896/5‑141 (MQ=11)
                                                                                          ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                      >  2:184893/4‑141 (MQ=11)
                                                                                          ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                      <  1:140873/138‑1 (MQ=11)
                                                                                          ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGACCTGAAGAGACCTGCgg                      >  1:86939/4‑141 (MQ=11)
                                                                                           tttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                     >  1:229285/3‑141 (MQ=11)
                                                                                           tCTAATAAAAATAGAAGGTTTTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                       <  2:1698/141‑1 (MQ=255)
                                                                                           tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                       <  1:106863/141‑1 (MQ=255)
                                                                                            ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCGCTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                    >  2:269101/2‑141 (MQ=11)
                                                                                            ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCTTGTTAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGTAAATTCGTAGGTTTGATGAGACCTGCgggt                    >  2:296495/2‑139 (MQ=11)
                                                                                            ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTGGATCTGAAGAGACCTGCGGtt                    >  2:144807/2‑141 (MQ=11)
                                                                                            ttaataaAAAT‑GATGGTTCTGTTACAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                    >  2:319691/2‑141 (MQ=11)
                                                                                             taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑TTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                   <  2:295532/141‑1 (MQ=11)
                                                                                                      ttaaaGTTTCTGATGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat            <  2:134037/138‑1 (MQ=0)
                                                                                                      tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat            >  2:152680/1‑141 (MQ=32)
                                                                                                        gctgggtCTGTTGCAAAGGAAAAAAATATAGCTAACCACTAATTTATCATATCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat          >  2:263720/7‑141 (MQ=0)
                                                                                                        aaaGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtttata         <  2:217198/140‑1 (MQ=2)
                                                                                                                tgttaaaaaGTAAAAAAATATAGCTAACCACTAATTTAGCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTa  <  2:114869/135‑1 (MQ=0)
                                                                                                                                           |                                                                                                                  
TCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCG‑CTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTA  >  CP000730/35711‑35963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑