Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 990,792 A→G 11.4% A228A (GCA→GCG)  oppD1 → oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730990,7920AG11.4% 82.4 / 4.7 39A228A (GCA→GCG) oppD1oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (17/17);  new base G (3/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01

ACCAACGACTGCATTAGACGTAACGATGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACACCATTACTAGCGATT  >  CP000730/990654‑990930
                                                                                                                                          |                                                                                                                                          
aCCAACGACTGCATTAGACGTAACGATGCAGGCACAAATTTTAGATTTAGTGAAGGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGt                                                                                                                                          >  2:43016/1‑141 (MQ=255)
aCCAACGACTGCATTAGACGTAACGATGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCAAGATTTAGGGGTAGTTGCGAATATTGCTGATAGAGAGGCAGt                                                                                                                                          >  2:49716/1‑141 (MQ=255)
        cTGCATTAGACGTAACGATGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTtatgtatg                                                                                                                                  <  1:283944/141‑1 (MQ=255)
                         aTGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTAtggtgg                                                                                                                              >  1:340390/1‑128 (MQ=255)
                         aTGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTAtggtgg                                                                                                                              <  2:340390/128‑1 (MQ=255)
                          tGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATCTAGCTCATTGAGTGGCGGTTATGTATGGTGGTCAAATGGTTGaaa                                                                                                                >  1:154211/1‑141 (MQ=255)
                           gCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAAc                                                                                                               <  2:80559/141‑1 (MQ=255)
                                  caAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGAt                                                                                                        <  1:18355/141‑1 (MQ=255)
                                   aaattaaatagttATTGAAAGAACTACAAAAAAAAGTCGATACAGCAATTTTTTTTATAACGAATGATTTAGGGGTTCTTTCGAATATGGCTGAGAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACATGAGATg                                                                                                       <  2:12565/130‑1 (MQ=255)
                                      ttttAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCGGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTa                                                                                                    >  1:192646/1‑141 (MQ=255)
                                      ttttAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCGGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTa                                                                                                    >  1:284227/1‑141 (MQ=255)
                                          aGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGGTATGTATGGTGGGCAAATGGTTGAAACGGGAGATGTTAACGa                                                                                                >  1:24061/1‑141 (MQ=255)
                                                aaTGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGCGTTGCAGAGGGGTATGGTGGTCAAAGGGTTGAAACAGGAGATGTTAACGAAATAtt                                                                                          >  1:169110/1‑141 (MQ=255)
                                                 aTGAAAGAACCACAACAAAAAATCGATACGGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATAttt                                                                                         <  2:114273/141‑1 (MQ=255)
                                                          cTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCa                                                                                >  1:199010/1‑141 (MQ=255)
                                                           taacacaaAAAATCGACACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCaa                                                                               <  1:238042/137‑1 (MQ=255)
                                                           tACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCGGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCaa                                                                               <  2:192646/141‑1 (MQ=255)
                                                           tACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCGGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCaa                                                                               <  2:284227/141‑1 (MQ=255)
                                                           aaacacaaAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCaa                                                                               <  1:103414/137‑1 (MQ=255)
                                                           aaaaaaaaaaaaTCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCaa                                                                               <  2:68334/135‑1 (MQ=255)
                                                                caaAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGGGATGTTAACGAAATAGTTTATGATTCAAAGCaa                                                                          >  2:226613/1‑140 (MQ=255)
                                                                    aaaTCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCat                                                                      <  1:6657/141‑1 (MQ=255)
                                                                       tCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCAgata                                                                   >  1:301576/1‑141 (MQ=255)
                                                                         gATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACa                                                                 >  1:308604/1‑141 (MQ=255)
                                                                         gATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACa                                                                 >  1:27834/1‑141 (MQ=255)
                                                                                   tgctttttAAAACGCATGATTTAGGGGTTGCTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGCTCCAAAGCAACCATATACATGGGGattat                                                       <  2:289798/138‑1 (MQ=255)
                                                                                      tttttATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATtattat                                                    <  2:199010/141‑1 (MQ=255)
                                                                                         ttATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGt                                                 >  1:153258/1‑141 (MQ=255)
                                                                                            taacgattgaagtAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCaa                                              <  2:249928/129‑1 (MQ=255)
                                                                                              aCGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCGATg                                            >  1:13918/1‑141 (MQ=255)
                                                                                                     aTTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGAtt                                     >  1:15572/1‑141 (MQ=255)
                                                                                                        tAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAGGCATGAATATACAAGGGGAGTATTATCGTCAATGCCTGATTTAt                                  >  2:195333/1‑141 (MQ=255)
                                                                                                           gggTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATACATATACATGGCGACTATTATCGTAAAGCCCTGATTTCTcaa                               >  2:316909/1‑141 (MQ=255)
                                                                                                            ggttgcttggAATATTGCTGAGAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATTATCCAAATCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATcaac                              <  2:2044/132‑1 (MQ=255)
                                                                                                                           tggtGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACAcc               <  2:154211/138‑1 (MQ=255)
                                                                                                                           tGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACAcc               <  2:24061/141‑1 (MQ=255)
                                                                                                                            gCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACACCa              >  1:216017/1‑141 (MQ=255)
                                                                                                                                      tGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACACCATTACTAGCGa    <  1:213582/141‑1 (MQ=255)
                                                                                                                                        gCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATACAAAGCATCCATATACATGGGGATTATTATGGTCAATGCCTGATTTATCAACAACAAATGACACACCATTACTAGCGAtt  >  1:162909/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACCAACGACTGCATTAGACGTAACGATGCAGGCACAAATTTTAGATTTAATGAAAGAACTACAACAAAAAATCGATACAGCAATTATTTTTATAACGCATGATTTAGGGGTTGTTGCGAATATTGCTGATAGAGTGGCAGTTATGTATGGTGGTCAAATGGTTGAAACAGGAGATGTTAACGAAATATTTTATGATCCAAAGCATCCATATACATGGGGATTATTATCGTCAATGCCTGATTTATCAACAACAAATGACACACCATTACTAGCGATT  >  CP000730/990654‑990930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑