Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,002,200 T→G 100% M173R (ATG→AGG)  mecA2 → competence negative regulator MecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,002,2000TG100.0% 50.4 / NA 16M173R (ATG→AGG) mecA2competence negative regulator MecA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/10);  total (6/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAAC  >  CP000730/1002063‑1002311
                                                                                                                                         |                                                                                                               
aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                              <  1:147397/141‑1 (MQ=255)
aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAAGCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCTTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                              <  2:2409/141‑1 (MQ=255)
aGGTGCTAAACGTCAAAAGTCTTCTGCACGGAAAAATACAAGAACAAGCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                              <  2:354349/141‑1 (MQ=255)
aGGTCCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                                              <  2:132350/141‑1 (MQ=255)
  gTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTg                                                                                                            >  1:278318/1‑141 (MQ=255)
     cTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTAGTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATg                                                                                                         <  1:346759/141‑1 (MQ=255)
         aCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTAc                                                                                                     >  2:334402/1‑141 (MQ=255)
                      tCAGCACGTAAAAATACAAGAACAATCATTGTCAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACACAGTTTCAAGATTTGTTATATAGGCTTGATGGTACTTATTATTATGCt                                                                                        <  2:187415/141‑1 (MQ=255)
                               aaaaaTACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtttt                                                                               >  2:282840/1‑141 (MQ=255)
                                aaaaTACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTg                                                                              <  2:284651/141‑1 (MQ=255)
                                aaaaGACAAGAATAATGATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTg                                                                              <  2:1649/141‑1 (MQ=255)
                                       aaGAACAATTATTGTGAAATTCAACGATTTAGAAGATGTTATTAATTATACCTTGCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCa                                                                       <  1:277662/141‑1 (MQ=255)
                                                                                  aattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGTTCCAGCGGCTCTTAAGTATAGTTCCGGTCAATCGCtt                            >  1:349932/1‑141 (MQ=255)
                                                                                                   cAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAac           >  2:315842/1‑141 (MQ=255)
                                                                                                        cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacagaca      <  1:284615/141‑1 (MQ=255)
                                                                                                            aaCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTGTCCAACAGacagaac  >  1:115583/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                               
AGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAAC  >  CP000730/1002063‑1002311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: