Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,105,9940TC100.0% 90.2 / ‑6.5 30T12T (ACT→ACCpdhDdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (14/14);  minor base A (1/0);  total (15/15)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTT  >  CP000730/1105867‑1106121
                                                                                                                               |                                                                                                                               
gACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                    <  1:132302/141‑1 (MQ=255)
  cAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCa                                                                                                                  <  1:98167/141‑1 (MQ=255)
    aaTTGGTGGTGCAACTGGCCAAAATACAATGAATCACACTAAATGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATCTCCCAATCGAAACAGATACCATCGTAATCGGAGCAgg                                                                                                                <  2:267543/141‑1 (MQ=255)
      ttGATGGTGCAACTTGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTc                                                                                                              <  2:101433/141‑1 (MQ=255)
       ttaTGTTGCAACTGGCCAAAATGGAATGAATCACAGTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAACCAGATACCATAGTAATCGGAGCAGGTcc                                                                                                             <  2:211871/139‑1 (MQ=255)
                     gCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTg                                                                                               <  2:271774/141‑1 (MQ=255)
                        aaaaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGAAAGAGAAGAACTTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcag                                                                                            <  1:260767/141‑1 (MQ=255)
                         aaaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcagc                                                                                           >  1:48317/1‑141 (MQ=255)
                                aTGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTCGCAGCAATTCGt                                                                                    >  2:105366/1‑141 (MQ=255)
                                 tGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCGTGGTGGATACGTTGCAGCAATTCGTg                                                                                   <  2:222646/141‑1 (MQ=255)
                                          ttAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAAt                                                                          <  1:79939/141‑1 (MQ=255)
                                             aaCGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAAGTTGAAGCAATTCGTTCAGcgccgttag                                                                       >  1:356096/1‑133 (MQ=255)
                                                         ataatCCAGAATTATTATTAAGGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGATCAATTAGGAAAAAAAGTaa                                                           >  1:158731/1‑141 (MQ=255)
                                                                      attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaa                                              >  2:326198/1‑141 (MQ=255)
                                                                      attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACAATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCGGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaa                                              >  2:347/1‑141 (MQ=255)
                                                                         attaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGGGCAGGTCCTGGTGGATACATTGCAGAAATTCGTGCAGCTCAATAAGGACAAAAAGTAACAATCGTTCAGCAAgg                                           >  2:356950/1‑141 (MQ=255)
                                                                          ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAACCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGCGAAAGGt                                          >  2:48114/1‑141 (MQ=255)
                                                                               ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCt                                     >  1:300348/1‑141 (MQ=255)
                                                                               ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCt                                     >  1:300309/1‑141 (MQ=255)
                                                                                   gggTAAAACATTGTAATTGGCGGTTTCCCAAATGAAACCTATACGATAGTAATCGGGGCAGGTCGTGGTGAATACGTTCCAGCAAGTCGCGCAGCTCAATGATGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggt                                 <  2:154039/141‑1 (MQ=255)
                                                                                      tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACGATCGTTGAGAAAGGTAATCTTggtggt                              >  2:104641/1‑141 (MQ=255)
                                                                                        aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGGGgtgt                            >  1:166784/1‑141 (MQ=255)
                                                                                        aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAAGCTTGgtggtgg                            >  2:242045/1‑140 (MQ=255)
                                                                                           cATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTg                         <  1:287649/141‑1 (MQ=255)
                                                                                           cATGCTAGTTGGTGCTTGCCTGACTGTAACAGATATCATAGTAAGCGGTGCCGGAACTGGTGGATACGTTGCAGCAATCCGTGCAGCTCCATTAGGCCAAATAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTg                         <  2:12434/141‑1 (MQ=255)
                                                                                            aTGGTAGTTTTAAGTTTCCAAAATGGAACAGATACCTCTGTTATCGGAATAATTCCTGTATTATACGATGCAGCAATTCGCGCAGCTCTATTAGGACAAAAAGTACCTATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                        <  2:267883/141‑1 (MQ=255)
                                                                                            aTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                        >  2:253865/1‑141 (MQ=255)
                                                                                                  attGGAGATTTTACTATTCAAACAGATACCAAAGAAATCGGAGCAGGTCCTGGTGGATAAGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAAc                  <  2:117430/140‑1 (MQ=255)
                                                                                                                 aTTGAAACAGATACCATAGTAATCGTAGCATGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCt   <  1:96397/141‑1 (MQ=255)
                                                                                                                  ttGAAACAGATACCATAGTACTCGGAACAGATCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTATTCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCtt  >  1:263854/1‑141 (MQ=255)
                                                                                                                               |                                                                                                                               
GACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTT  >  CP000730/1105867‑1106121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: