| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,194,918 | A→G | 11.7% | intergenic (+973/‑2819) | ftsQ → / → ylmE | cell division protein FtsQ/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,194,918 | 0 | A | G | 11.7% | 79.5 / 2.5 | 34 | intergenic (+973/‑2819) | ftsQ/ylmE | cell division protein FtsQ/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base G (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ACACTTCTTATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAATTATTA > CP000730/1194781‑1195058 | acacTTGTTATGAAACTGCTGAAAAAGTTAAACACCGATATGGTCATGCATTATATGAATCTGCTTCAGATCAAGATATATTCTCTGTTAAACAGGTTGATAGTGATAAAACAGAACAGTATACTCAAAAAAATTTTAGTg > 2:253619/1‑141 (MQ=255)acacTTCTGATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTCTCGTAGACTCAAAAAGATTTGAGTg < 1:85982/141‑1 (MQ=255) tGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGa < 2:303370/141‑1 (MQ=255) aaCTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAgc < 2:108558/141‑1 (MQ=255) tgAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCgtgt > 1:110360/1‑141 (MQ=255) ttAACCACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGaaa < 1:213805/141‑1 (MQ=255) aaaCACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAata < 1:209414/141‑1 (MQ=255) aaTATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAGATATTCTTCg > 1:118892/1‑141 (MQ=255) tatGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGaa > 1:93189/1‑141 (MQ=255) gCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATCCTGACTAAGTTGTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGAt > 1:260410/1‑141 (MQ=255) gCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGAt < 1:359002/141‑1 (MQ=255) ttCTATGATTCTGCTTCAGATCAAGATATCCTCACTGTTAGACAGTTGTATAGGGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGtt < 1:333956/141‑1 (MQ=255) cTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGtttt > 1:35913/1‑141 (MQ=255) aTTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAg < 1:54051/141‑1 (MQ=255) gCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTa > 1:118444/1‑141 (MQ=255) gCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGAGTTa > 1:311577/1‑141 (MQ=255) aTCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTaa > 2:228774/1‑141 (MQ=255) cAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACa > 2:243319/1‑141 (MQ=255) caGATATCTTCACTGTTGAACAGGTTCATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACaa < 1:216757/140‑1 (MQ=255) tatCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATGTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGt > 2:277394/1‑141 (MQ=255) tCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAAtg < 2:142245/141‑1 (MQ=255) cTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTCACAAAAGTAAAtggtg < 1:212541/141‑1 (MQ=255) ttGAACAGGTTGAGAGTGATGAACCAGTACAGTATAATCAAAGGGAATTGAGTGGTTTAAGAGAAGCGCGTGTAGAAGAAATATGCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGt < 2:74858/141‑1 (MQ=255) gTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTa < 2:311058/141‑1 (MQ=255) ttGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa > 1:113641/1‑141 (MQ=255) ttGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa > 1:198458/1‑141 (MQ=255) ttGATAGTGATGAAACAGTACAGTACACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa > 1:179598/1‑141 (MQ=255) aaCAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGc < 2:296638/141‑1 (MQ=255) caggacaggaTACTCAAAAAGATTTGAGGGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCaa > 2:263619/1‑141 (MQ=255) tacagtaTACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAact > 2:160143/1‑141 (MQ=255) tgcAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCg < 2:118892/139‑1 (MQ=255) aaaaaGATTTGGGTGTCTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGGaa < 2:311577/141‑1 (MQ=255) aaaaaGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTaa < 2:118444/141‑1 (MQ=255) aTTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAat > 1:295041/1‑141 (MQ=255) aGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAAttatta > 1:135048/1‑141 (MQ=255) | ACACTTCTTATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAATTATTA > CP000730/1194781‑1195058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |