Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,513,930 A→T 100% L413F (TTA→TTT)  USA300HOU_1379 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,513,9300AT100.0% 82.2 / NA 26L413F (TTA→TTT) USA300HOU_1379hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/10);  total (16/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAA  >  CP000730/1513807‑1514067
                                                                                                                           |                                                                                                                                         
tatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACTGCAGTTAGAGAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattatt                                                                                                                          <  1:155803/141‑1 (MQ=255)
        tattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACAAGCCTCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCat                                                                                                                  >  2:97841/1‑141 (MQ=255)
                      ggCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCa                                                                                                    >  1:315849/1‑141 (MQ=255)
                       gCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATGCTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCCTTATTTGGATTATCAc                                                                                                   >  1:157820/1‑141 (MQ=255)
                       gCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCAc                                                                                                   >  1:74412/1‑141 (MQ=255)
                         atggcatttGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATAGCGTAAAAAAACCAGCATCTATATCACCCATAACATGTTTTATTGTATTAGCATTATTAAGGCCATTATTTGGATTATCACtt                                                                                                 <  2:108093/135‑1 (MQ=255)
                           ggggtttgcgcaatGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCAGCTATAGCTCTCATATTTTGTTTTATTGTATTAGGTTTATTAATGCCATTATTTGGATGATCACTTAt                                                                                               <  2:139944/128‑1 (MQ=255)
                                ttGTCTCTTGGATAAAGCGTGCAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTGTATTGTATTAGGATTATTAATGCAATTATTTGGATTATCTCTTATCCTTg                                                                                          >  2:174971/1‑141 (MQ=255)
                                  ctctcTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTAGATTATCACTTATCCTTGTa                                                                                        <  1:218600/140‑1 (MQ=255)
                                            tAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTa                                                                                        <  2:310674/131‑1 (MQ=255)
                                            tAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTa                                                                                        >  1:310674/1‑131 (MQ=255)
                                              aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGat                                                                            >  1:171093/1‑140 (MQ=255)
                                              aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                            >  1:336770/1‑141 (MQ=255)
                                               aGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAt                                                                           >  1:261244/1‑141 (MQ=255)
                                                  gTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTaa                                                                        >  2:348916/1‑141 (MQ=255)
                                                       aacaaTACTCCAATAAAAGTACCACATCGCGTAAAATAACCATCATCTTTATCTCTTATAATTTGTTTTATTGTATTAGTATTATTAATGCCATTATTTTGATTATCTCTTATCCTTGTATTTATAATTGAATTAAtatta                                                                   <  2:171093/138‑1 (MQ=255)
                                                       aaaaaTACTGCAATAAAAGTAACACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCCCGTAGTTATAATTGAATTAAtatta                                                                   >  2:161058/1‑141 (MQ=255)
                                                                 cAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaaaa                                                         >  1:331296/1‑140 (MQ=255)
                                                                          tACCACCTCGCGTGAAAAAACCAGCATCTAGATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                <  2:319569/141‑1 (MQ=255)
                                                                                           aaaCCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACt                               <  1:202490/141‑1 (MQ=255)
                                                                                           aaaCCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGACATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACt                               <  1:28920/141‑1 (MQ=255)
                                                                                             aacagctccTAGATCCCTTATAATATTGTTTATTGTATTAGGATTATTAATCCCATTATTTGGCTTATCACTTATCCTTGTAGTTATAATTGAATTAATATTATATATTAAATACCATCCTGCTAAACAATAATGCACTTa                             <  2:92503/133‑1 (MQ=255)
                                                                                              ccAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTaa                            >  2:62407/1‑141 (MQ=255)
                                                                                               cAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTaaa                           >  2:162446/1‑141 (MQ=255)
                                                                                                                   atatGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTATAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt       >  1:151908/1‑141 (MQ=255)
                                                                                                                   atatGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt       >  1:234617/1‑141 (MQ=255)
                                                                                                                        ttttATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACaaa  <  1:183300/141‑1 (MQ=255)
                                                                                                                           |                                                                                                                                         
TATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAA  >  CP000730/1513807‑1514067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑