Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,680,793 A→G 11.5% I15I (ATT→ATC dgkA2 ← diacylglycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,680,7930AG11.5% 81.4 / 4.3 35I15I (ATT→ATCdgkA2diacylglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (16/15);  new base G (2/2);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TAAAGCTTCAACAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCAGTCATATGATCATAACCTAAT  >  CP000730/1680655‑1680933
                                                                                                                                          |                                                                                                                                            
tAAAGCTTCAACAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAAt                                                                                                                                            >  1:356165/1‑141 (MQ=255)
  aaGCTTCAACAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGGATTa                                                                                                                                          >  2:332427/1‑141 (MQ=255)
  aaGCTTCAACAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGGATTa                                                                                                                                          >  2:301748/1‑141 (MQ=255)
           cAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCa                                                                                                                                 >  1:128548/1‑141 (MQ=255)
                   aCTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCa                                                                                                                         >  2:51938/1‑141 (MQ=255)
                    cTAATGCAATAGCAATGGGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCaa                                                                                                                        <  1:29765/141‑1 (MQ=255)
                    cTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCaa                                                                                                                        <  1:206632/141‑1 (MQ=255)
                         gCAATAGCAATGAGTATAAATATCCACTCAATCTGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCa                                                                                                                   >  1:121138/1‑141 (MQ=255)
                            aTAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCatat                                                                                                                <  1:142953/141‑1 (MQ=255)
                             tAGCAATGAGTATAAATATCCACTCAATCCGATTAATGTTTAGTACGAGACCAAAGACAGTCTCAACAATCATAGCAGACGCATGTAATAGGAAGCTATGGTCTTTTGGATTTAAGAGTTTACGCCCATCACGTGCATAtt                                                                                                               >  2:8053/1‑141 (MQ=255)
                              aGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATAttt                                                                                                              >  2:286749/1‑141 (MQ=255)
                                      gTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACtttt                                                                                                      >  2:22690/1‑141 (MQ=255)
                                        ataAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCTTATTTAAACCTTTTc                                                                                                    >  2:191606/1‑141 (MQ=255)
                                                     cAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACACGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCg                                                                                       >  2:239718/1‑141 (MQ=255)
                                                       aTCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCgtg                                                                                     >  1:252815/1‑141 (MQ=255)
                                                          cGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAGACCTTTTCATAATTAGTCTCGTGTTa                                                                                  >  1:161826/1‑141 (MQ=255)
                                                            aTTAATATTTAGTACGAGACCAAAGACAATAGCAACCATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAAt                                                                                <  1:8856/141‑1 (MQ=255)
                                                             ttAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATc                                                                               <  1:242952/141‑1 (MQ=255)
                                                                     tAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCg                                                                       <  1:326005/141‑1 (MQ=255)
                                                                        tACGAGACCAAAGACAATAGCAACAACCATTGTAAACACATGTACCCGAAATTTAGAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCTATATGCGttt                                                                    <  1:259248/141‑1 (MQ=255)
                                                                             gaCCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGGATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAatat                                                               <  1:332427/141‑1 (MQ=255)
                                                                             gaCCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGGATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAatat                                                               <  1:301748/141‑1 (MQ=255)
                                                                               ccAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTg                                                             <  1:129125/141‑1 (MQ=255)
                                                                                      cAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATCATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTg                                                      <  1:75506/141‑1 (MQ=255)
                                                                                         tAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGGCATTTTGAAGTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCg                                                   >  1:246034/1‑141 (MQ=255)
                                                                                                     ttGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTc                                       <  2:143105/141‑1 (MQ=255)
                                                                                                     tggCAAACACATGTAAAACAAATTTAGAGTCTTTTTGAATTAAGATTTTCAGCCCATTAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTc                                       <  2:173492/139‑1 (MQ=255)
                                                                                                               aTGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATcc                             <  1:215909/141‑1 (MQ=255)
                                                                                                                tGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCg                            <  2:159702/141‑1 (MQ=255)
                                                                                                                 gTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATGGTATCTTTTCGACCAAACATGTCCTTTTGATcctc                           >  1:342777/1‑139 (MQ=255)
                                                                                                                  tAAAAGACATTTATAGTCTTTTTGAATTAAGATTTTCAACCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCt                          <  1:239209/141‑1 (MQ=255)
                                                                                                                     aaGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCa                       <  1:239718/141‑1 (MQ=255)
                                                                                                                               taGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTACATATTTAAAACTTTTCATAATTCGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAGCATTTCCTTCTCATCCGCTTCAGTCATATGAt             >  1:247691/1‑141 (MQ=255)
                                                                                                                                     ttttGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCAGTCATATGATCATAAc       >  1:336561/1‑141 (MQ=255)
                                                                                                                                         gAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCAGTCATATGATCATAACCTaa   >  2:358790/1‑141 (MQ=255)
                                                                                                                                          aaTTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCAGTCATATGATCATAACCTAAt  >  2:63766/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TAAAGCTTCAACAGTGAGAACTAATGCAATAGCAATGAGTATAAATATCCACTCAATCCGATTAATATTTAGTACGAGACCAAAGACAATAGCAACAATCATTGCAAACACATGTAAAAGAAATTTATAGTCTTTTTGAATTAAGATTTTCAGCCCATCAAGTGCATATTTAAACCTTTTCATAATTAGTCTCGTGTTAATCCATATGCGTTTAATATTGTATCTTGTCGACCAAACATTTCCTTTTCATCCGCTTCAGTCATATGATCATAACCTAAT  >  CP000730/1680655‑1680933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: