Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4730CT100.0% 80.5 / NA 27A67T (GCT→ACT) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAT  >  CP000730/1744336‑1744596
                                                                                                                                         |                                                                                                                           
ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTAc                                                                                                                          <  1:198020/141‑1 (MQ=255)
 tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACa                                                                                                                         <  2:138082/141‑1 (MQ=255)
   aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATCCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                       <  1:205894/141‑1 (MQ=255)
      tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACa                                                                                                                    <  1:194218/141‑1 (MQ=255)
       ggTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAg                                                                                                                   <  2:214120/141‑1 (MQ=255)
                          gCATTGTTAACACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGACAggcg                                                                                                >  1:301767/1‑141 (MQ=255)
                          gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                                >  1:301761/1‑141 (MQ=255)
                                  aaaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt                                                                                        >  2:272431/1‑141 (MQ=255)
                                    aaCATTGGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                                      <  1:238744/141‑1 (MQ=255)
                                     aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                     >  1:91630/1‑141 (MQ=255)
                                         ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                                 <  1:251709/141‑1 (MQ=255)
                                           gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGTTATTTTACCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTTAATAAGTTACAGGACAGCCAATGATAAAGCCACGCACTTCAGGTCCTACAACg                                                                               >  1:139280/1‑141 (MQ=255)
                                           gTACCATATACTAGGTCATACTCATAACGAATGACTTCACGAGGTAACTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                                               >  2:230589/1‑141 (MQ=255)
                                                   ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                                                       <  1:67634/141‑1 (MQ=255)
                                                   ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                                                       <  1:110659/141‑1 (MQ=255)
                                                   gtCTTGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                                                       <  2:357684/140‑1 (MQ=255)
                                                          tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACATGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                <  2:237615/141‑1 (MQ=255)
                                                          tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                <  2:325534/141‑1 (MQ=255)
                                                          tCATACTCAAAACTAAAGACTGCACGACGTAATTTCCCCTCTTTTCTAACAGCTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                <  2:2630/141‑1 (MQ=255)
                                                                          tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTTCCATGCCATTCCCCATTGAATAATTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATCTCTGTCTTTTGGGTATTCTa                                                >  1:134809/1‑141 (MQ=255)
                                                                            aCTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCACCATTGAATAAGTTACAGGACAGCCAGTGATAAAGCCAAGCGCTTCAGGTCCTTCAACGATATCAACATCTCTGTCTTTTTCTTATGCAACa                                              >  2:36609/1‑141 (MQ=255)
                                                                             cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                                             >  1:278712/1‑141 (MQ=255)
                                                                                  cggggTAATTTCCCTTCTTTTCTAACAGGGTCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa                                        <  2:193200/138‑1 (MQ=255)
                                                                                    aGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATc                                      >  1:194740/1‑141 (MQ=255)
                                                                                                              gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt            <  1:326783/141‑1 (MQ=255)
                                                                                                                  aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCACTGATAAAGCAACGCACATCAGGTCCTACAACGATATCAATATCTCTGTCTTTTGCGTATTCAACAATTTTCTCTGTTGCATAGCCATATGCTTCACCATTATc        >  2:140266/1‑141 (MQ=255)
                                                                                                                        aaTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAt  >  1:140046/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                           
TTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAT  >  CP000730/1744336‑1744596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: