| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,455,692 | Δ1 bp | coding (1210/1260 nt) | USA300HOU_2315 → | hypothetical membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,455,692 | 0 | G | . | 91.4% | 224.6 / 16.7 | 60 | coding (1210/1260 nt) | USA300HOU_2315 | hypothetical membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base . (23/30); minor base A (3/2); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTT > CP000730/2455557‑2455824 | tCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaa < 1:119536/141‑1 (MQ=255) agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTACTTGATGCAaaaaaatt > 1:207158/1‑139 (MQ=255) aaTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcga > 1:1298338/1‑134 (MQ=255) aTCAGGTAAACAAGCCCCACAACGTGACGATTTAAAAAACGATTCGTTAAGCA‑ATGGCATATAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcgaa < 1:39692/141‑9 (MQ=255) cAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAATACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaa < 1:1364591/141‑1 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaaa > 1:94459/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATGGAAAAAACTGAAGAAc < 1:818036/141‑1 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc < 1:603341/141‑1 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc < 1:1097068/141‑1 (MQ=255) gCACCACAAAGTCACGATTTAACAAACGATTC‑AAATAAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc < 1:1230195/141‑1 (MQ=255) acaccacAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGACCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc < 1:793492/140‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAGGAACCTGCGACTTATAAAGAAGACTGACGTTCATCAGTTGTAATTGATGC‑TAAAAAATGATCGAAAAAACTGAAGAACa < 1:890633/141‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACa < 1:444311/141‑1 (MQ=255) caccacAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCAGTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACa < 1:862574/141‑1 (MQ=255) gACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGCAAAAACTGAAGAACAATCTTCAGATa > 1:331410/1‑141 (MQ=255) aCGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAACAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAGCTGAAGAACAATCTTCAGATaa < 1:1293767/141‑1 (MQ=255) aCGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaa < 1:683569/141‑1 (MQ=255) aCGATTTAACAAACGATTC‑AAATGAAGAGGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaa < 1:180822/141‑1 (MQ=255) gATTTAACAAACGATTC‑AAATGAAGATGAACTAGAGCAATCATTCAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCCGATaaaa > 1:814360/1‑141 (MQ=255) gATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa < 1:201554/141‑1 (MQ=255) aTTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaaa < 1:1190288/141‑1 (MQ=255) tttAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc < 1:1004576/141‑1 (MQ=255) tataataaACTATTA‑AACTGCAGAAGCAATAAATCATGCTTTAAAAGTACCATTGAATTATAAAGAAGACTGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAc < 1:1320971/139‑1 (MQ=255) aCAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat < 1:413632/141‑1 (MQ=255) aCAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat < 1:499586/141‑1 (MQ=255) cAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaata > 1:1177740/1‑141 (MQ=255) aCGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata > 1:1256976/1‑141 (MQ=255) c‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAGAATGAATA‑CGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaaa > 1:1069583/1‑140 (MQ=255) c‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg > 1:1262396/1‑141 (MQ=255) aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGc < 1:862621/141‑1 (MQ=255) aGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGGTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGc < 1:862413/141‑1 (MQ=255) aGATGAAATAGAGCAATCATGAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACACATAATAACAATGCGCTTGc < 1:677483/141‑1 (MQ=255) aTGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACATACGTTCATCAGTTGTAATTGATGCAGAAAAA‑TTATCGAAAAAACTGAAGAATAATCTTCACATAAAAACATATAATAACAAAGCGCCTGCTa < 1:1014930/141‑1 (MQ=255) gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGt > 1:152947/1‑141 (MQ=255) gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCCGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct < 1:258373/141‑1 (MQ=255) ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTACATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTAAAAAgat > 1:954113/1‑141 (MQ=255) aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAcatga > 1:1308501/1‑141 (MQ=255) aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAgatga > 1:1284921/1‑141 (MQ=255) aGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGc < 1:812025/141‑1 (MQ=255) gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaaaacgagacc > 1:162422/1‑132 (MQ=255) ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc > 1:781849/1‑141 (MQ=255) cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 1:614321/141‑1 (MQ=255) tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa > 1:1217678/1‑141 (MQ=255) tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa > 1:447364/1‑141 (MQ=255) tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACCGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTACCaa > 1:673007/1‑141 (MQ=255) cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc > 1:748490/1‑141 (MQ=255) gACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAACAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg < 1:121736/141‑1 (MQ=255) aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTCCCACTTAAAACGATGATGCTAGCAAgcgc > 1:1197425/1‑141 (MQ=255) cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt > 1:847748/1‑141 (MQ=255) ttATAAACAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt < 1:23972/141‑1 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCACGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt < 1:666729/141‑1 (MQ=255) taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc < 1:1175306/141‑1 (MQ=255) agacagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgggactttttatcct > 1:353521/1‑127 (MQ=255) agacagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatact > 1:1117105/1‑141 (MQ=255) cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGCTGCTAGCAAGCGCTTTTctatactata > 1:498587/1‑141 (MQ=255) cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata > 1:1226773/1‑141 (MQ=255) cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata > 1:943163/1‑141 (MQ=255) cagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGATTTTctatactata > 1:217996/1‑141 (MQ=255) acGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTatatat < 1:720646/141‑1 (MQ=255) gTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatta < 1:1006254/141‑1 (MQ=255) gTTCATCAGCTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatta < 1:797643/141‑1 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt < 1:19285/141‑1 (MQ=255) gTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTa < 1:195026/141‑1 (MQ=255) aTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTATTCTATACTATATATTATTTTTCTAAACttt > 1:1266620/1‑141 (MQ=255) | TCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTC‑AAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTT > CP000730/2455557‑2455824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |