Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,231,720	G→C	R122T (AGA→ACA)	prpC →	possible PP2C protein phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,231,720	0	G	C	100.0%	215.0 / NA	63	R122T (AGA→ACA)	prpC	possible PP2C protein phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/35);  total (28/35)

AATATAAAAGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTAGAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAGG  >  CP000730/1231587‑1231854                                                                                                                                      |                                                                                                                                       aataTAAAAGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTAt                                                                                                                                 >  1:497451/1‑141 (MQ=255)     taAAAGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGtta                                                                                                                             <  1:903869/141‑1 (MQ=255)       aaaGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGttatt                                                                                                                           <  1:286580/141‑1 (MQ=255)       aaaGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGttatt                                                                                                                           >  1:213732/1‑141 (MQ=255)       aaaGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGttatt                                                                                                                           >  1:130580/1‑141 (MQ=255)                aaTTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGa                                                                                                                  <  1:277644/141‑1 (MQ=255)                aaTTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGa                                                                                                                  <  1:1193987/141‑1 (MQ=255)                aaTTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGa                                                                                                                  <  1:1298954/141‑1 (MQ=255)                aaTTGTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGa                                                                                                                  <  1:608377/141‑1 (MQ=255)                 aTTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGAc                                                                                                                 <  1:825419/141‑1 (MQ=255)                     tCAGTTATATCACTAGGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAAt                                                                                                             <  1:1242785/141‑1 (MQ=255)                       aGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATCGTAGACAAATTg                                                                                                           >  1:1297907/1‑141 (MQ=255)                       aGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTg                                                                                                           >  1:127528/1‑141 (MQ=255)                       aGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATATTATTAATCGTCGAGAAAttt                                                                                                           >  1:93182/1‑140 (MQ=255)                                  tATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACt                                                                                                >  1:420508/1‑141 (MQ=255)                                          aGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCa                                                                                        <  1:53311/141‑1 (MQ=255)                                          aGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCa                                                                                        >  1:1183544/1‑141 (MQ=255)                                          aGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCa                                                                                        <  1:159216/141‑1 (MQ=255)                                          aGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGGGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCa                                                                                        >  1:451555/1‑141 (MQ=255)                                           aaaaaTGCAGAATATAAAGGTATGGTTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCAc                                                                                       <  1:427052/140‑1 (MQ=255)                                            aaaaTGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACt                                                                                      <  1:960105/141‑1 (MQ=255)                                                  cAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTg                                                                                <  1:547808/141‑1 (MQ=255)                                                      atatAAAGGTATGGGAAAAACCTATGTGGGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTaa                                                                            >  1:330539/1‑141 (MQ=255)                                                        ataAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTCGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAatc                                                                          >  1:1089873/1‑141 (MQ=255)                                                          tagggTATTGGTTCAACATGTTTTTTTGCACTTTTGTTTGCCAACTCATTTGGTACAGCCACTTTCGGTGATTCTACACCCTCTGTTATTACTAGTCGACAAATTTAACAAATTACTAGTGCTCACTCATTTGTTAatcat                                                                        <  1:744432/138‑1 (MQ=255)                                                          aaaGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAatcat                                                                        <  1:744438/141‑1 (MQ=255)                                                                 tGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAACTTACTAGTGATCACTCATTTGTTAATCATCTTGttt                                                                 >  1:521459/1‑141 (MQ=255)                                                                 tGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATGTTGttt                                                                 >  1:1247974/1‑141 (MQ=255)                                                                 tGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGttt                                                                 >  1:521518/1‑141 (MQ=255)                                                                 tGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGttt                                                                 >  1:1197426/1‑141 (MQ=255)                                                                   ggTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTa                                                               <  1:812032/141‑1 (MQ=255)                                                                       caacaTGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACgg                                                           >  1:987260/1‑141 (MQ=255)                                                                       caacaTGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACgg                                                           >  1:504498/1‑141 (MQ=255)                                                                       caacaTGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAAGTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTACCgg                                                           >  1:629079/1‑141 (MQ=255)                                                                         acaTGTGTTTGTGCACTTGTTTTTGAAAGATCAGTTGTGATAGCAAATGTCGGTAATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGt                                                         >  1:888411/1‑141 (MQ=255)                                                                         acaTGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGt                                                         >  1:1209888/1‑141 (MQ=255)                                                                             gtgtTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTCGTTTTAACGGGTCaaa                                                     <  1:764624/141‑1 (MQ=255)                                                                                        cTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaa                                          <  1:785704/141‑1 (MQ=255)                                                                                        cTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaa                                          <  1:481605/141‑1 (MQ=255)                                                                                        cTTGTTTTTGAAAAAGCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaa                                          <  1:41129/141‑1 (MQ=255)                                                                                        cTTGTTTTTGAAAAAACAGTCGGGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaa                                          <  1:79971/141‑1 (MQ=255)                                                                                        cTTGTTTTGGAAAAATCAGTTGTGATAGCAAATGTGGGTGATTCTACAGCCTATGTTATGAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaa                                          <  1:172635/141‑1 (MQ=255)                                                                                          tGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGgaaga                                        <  1:1249405/141‑1 (MQ=255)                                                                                                tGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCAtt                                  <  1:762811/141‑1 (MQ=255)                                                                                                 gAAAAAGCAGTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGAGCACTCATTTGTTAGTCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCAttt                                 <  1:658610/141‑1 (MQ=255)                                                                                                       tctgTTGTGATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACAt                           <  1:1187526/138‑1 (MQ=255)                                                                                                            tgtgATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAACTTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCcaca                      <  1:290737/141‑1 (MQ=255)                                                                                                            tgtgATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCcaca                      <  1:1091073/141‑1 (MQ=255)                                                                                                            tgtgATAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCcaca                      <  1:303099/141‑1 (MQ=255)                                                                                                                aTAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCTACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGt                  <  1:1287781/141‑1 (MQ=255)                                                                                                                aTAGCAAATGTCGGGTACTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGt                  <  1:574039/141‑1 (MQ=255)                                                                                                                 tAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAACTTGAGCTAATTACTAGTGATCACTCATTTGTTAATCGTCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACATCGTa                 <  1:938421/141‑1 (MQ=255)                                                                                                                 tAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTa                 <  1:1111851/141‑1 (MQ=255)                                                                                                                 tAGCAAATGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTa                 <  1:221978/141‑1 (MQ=255)                                                                                                                        tGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAGGCATTTACACATCCACAACGTAAtattat          >  1:1174985/1‑141 (MQ=255)                                                                                                                        tGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAAtattat          >  1:215305/1‑141 (MQ=255)                                                                                                                        tGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAAtattat          >  1:542669/1‑141 (MQ=255)                                                                                                                        tGTCGGTGATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCAGGAAGAAGCATTTACACATCCACAACGTAAtattat          >  1:487505/1‑141 (MQ=255)                                                                                                                               gATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAg   >  1:546469/1‑141 (MQ=255)                                                                                                                               gATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAg   >  1:739885/1‑141 (MQ=255)                                                                                                                               gATTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAg   >  1:1284419/1‑141 (MQ=255)                                                                                                                               gATTCTACAGCCTATGTTATAAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAAAGGGTCAAATTACGCCGGAAGAAGCATTTACACATTCACAACGTAATATTATTACGAAg   >  1:868235/1‑141 (MQ=255)                                                                                                                                aTTCTACAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAgg  <  1:738722/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       AATATAAAAGATATAAATTTTCAGTTATATCACTATGCACAAGAAAATGCAGAATATAAAGGTATGGGTACAACATGTGTTTGTGCACTTGTTTTTGAAAAATCAGTTGTGATAGCAAATGTCGGTGATTCTAGAGCCTATGTTATTAATAGTCGACAAATTGAACAAATTACTAGTGATCACTCATTTGTTAATCATCTTGTTTTAACGGGTCAAATTACGCCGGAAGAAGCATTTACACATCCACAACGTAATATTATTACGAAGG  >  CP000730/1231587‑1231854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑