Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,744,473	C→T	A67T (GCT→ACT)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,473	0	C	T	100.0%	153.3 / NA	49	A67T (GCT→ACT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

GTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAA  >  CP000730/1744344‑1744604                                                                                                                                  |                                                                                                                                    gTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCAGc                                                                                                                          >  1:1138716/1‑141 (MQ=255)     aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCCAt                                                                                                                      >  1:672439/1‑141 (MQ=255)     aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAAt                                                                                                                      >  1:1224288/1‑141 (MQ=255)     aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAAt                                                                                                                      >  1:1223994/1‑141 (MQ=255)           aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATaaa                                                                                                                <  1:812269/141‑1 (MQ=255)           aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATaaa                                                                                                                <  1:255530/141‑1 (MQ=255)              tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGCGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGcc                                                                                                             <  1:624038/141‑1 (MQ=255)               ttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGcaa                                                                                                            >  1:1317606/1‑139 (MQ=255)                    cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                       <  1:560902/141‑1 (MQ=255)                    cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                       <  1:1053716/141‑1 (MQ=255)                                    gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCg                                                                                       >  1:135436/1‑141 (MQ=255)                                    gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                                                       >  1:262780/1‑141 (MQ=255)                                    gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                                                       >  1:790634/1‑141 (MQ=255)                                     tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTCCTTTTCTAACAAGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGCTAAAGCCACGCGCTTCAGGTCCTACAACGa                                                                                      >  1:261029/1‑141 (MQ=255)                                         atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCGatata                                                                                  >  1:1254303/1‑140 (MQ=255)                                             tCTAGGTCATACTCATAACGAATGACTTCACGCAGTAATTTTCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGACTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa                                                                              <  1:116789/141‑1 (MQ=255)                                                 ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCGTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                                          >  1:214939/1‑141 (MQ=255)                                                    cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                       <  1:1372534/141‑1 (MQ=255)                                                    cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                       <  1:1100410/141‑1 (MQ=255)                                                     atccTCATAACGAATGACTTCACGAGGGAATTTCCCTTCGTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACATGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                                      <  1:1261549/138‑1 (MQ=255)                                                     aTACTCATAACGAATGACTCCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                                      <  1:4593/141‑1 (MQ=255)                                                        cTCATAACGAATGACTTCACGAGGTAATTTCTCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                                   >  1:1155266/1‑141 (MQ=255)                                                                      cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATCTCTGTCTTTTGCGTATTCTAcct                                                     >  1:620453/1‑139 (MQ=255)                                                                         cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                  <  1:305754/141‑1 (MQ=255)                                                                         cACGAGGTAATTTCCCTTCTTTCCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                  <  1:387303/141‑1 (MQ=255)                                                                                  aTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                         <  1:1049119/141‑1 (MQ=255)                                                                                  aTTTCCCGTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAGAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                         <  1:52546/141‑1 (MQ=255)                                                                                   tttCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTCCAGTTTTATCTGTTGc                                        >  1:1365372/1‑141 (MQ=255)                                                                                          tcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCa                                 <  1:331375/141‑1 (MQ=255)                                                                                           cttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTCCACTTATATCTGTTGCATAGCCat                                >  1:82233/1‑141 (MQ=255)                                                                                           cttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCat                                <  1:201057/141‑1 (MQ=255)                                                                                           cttTTCTAACAGGTGAAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAGTGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTCCGTATTATAAAATTTTATCTTTTGCATAGCCat                                >  1:689834/1‑141 (MQ=255)                                                                                            ttttCTAACAGGTGCAAAGCNCNTCCCCATTGAATAAGTTACAGGTCAACCGATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                               <  1:783099/141‑1 (MQ=255)                                                                                            ctttCTAACAGGTGCAAAGCCAAGCCCCATTCAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                               <  1:12181/140‑1 (MQ=255)                                                                                                  aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                         >  1:523078/1‑141 (MQ=255)                                                                                                  aaCAGGTGCAAAGCCAATCACCATTCAATAAGTTACAGGACAGCAAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                         >  1:750192/1‑141 (MQ=255)                                                                                                           aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACACTTTTATCTGTTGCATAGCCATATGCTTCACCCTtctc                >  1:631308/1‑141 (MQ=255)                                                                                                           aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATc                >  1:1220762/1‑141 (MQ=255)                                                                                                            aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc               <  1:571143/141‑1 (MQ=255)                                                                                                            aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc               >  1:220280/1‑141 (MQ=255)                                                                                                            aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc               <  1:416881/141‑1 (MQ=255)                                                                                                             aGCCAATCCCCATTAAATAAGTTACAGGACAGCTAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa              <  1:477303/141‑1 (MQ=255)                                                                                                              gCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATATGTTGCATAGCCATATGCGTCACCATTATCCAt             >  1:478710/1‑141 (MQ=255)                                                                                                                    ccccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTCCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt       >  1:143681/1‑141 (MQ=255)                                                                                                                    ccccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCCTATGCTTCACAGTTATCGATACTTgt       >  1:361142/1‑141 (MQ=255)                                                                                                                     cccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACTATTATCCATAATTgta      <  1:763352/141‑1 (MQ=255)                                                                                                                         ttGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTaa  <  1:167473/141‑1 (MQ=255)                                                                                                                         ttGAATAAGTTACAAGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTaa  <  1:608718/141‑1 (MQ=255)                                                                                                                         ttGAATAAGTCACAAGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATTTTGTCAACTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATTATTGTAGTaa  <  1:1356874/141‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    GTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAA  >  CP000730/1744344‑1744604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑