| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,758,882 | G→A | 100% | Q138* (CAA→TAA) | USA300HOU_1650 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,758,882 | 0 | G | A | 100.0% | 13.0 / NA | 5 | Q138* (CAA→TAA) | USA300HOU_1650 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (1/4); total (1/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTGAACTACACCAACCTTTTAAATTTATTCTAGTGACAGGATAACTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTGCATTTCAAATTCAGTATTGTAAACACGCATTGACTCATCTCCAAATTTATAAAGAATAAATTCGTCTCCTCTTTGACCTATAATATATTGATCATTATAAGCCATGCGATTCATTCCAGACACAGCCATAAACTC > CP000730/1758746‑1759017 | tttGAACTACACCAACCTTTTAAATTTATTCTAGTGACAGGATAACTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTACAtt < 1:41692/141‑1 (MQ=255) cTACACCAACCTTTTAAATTTATTCTAGTGACAGGATAACTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTACATTTCAAAt < 2:62427/141‑1 (MQ=255) aTAACTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTACATTTCAAATTCAGTATTGTAAACACGCATTGACTCATCTCCaaa < 2:25451/141‑1 (MQ=255) aaCTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTACATTTCAAATTCAGTATTGTAAACACGCATTGACTCATCTCCAAAtt > 2:60320/1‑141 (MQ=255) aaGTCTTGTTACATTTCAAATTCAGTATTGTAAACACGCATTGACTCATCTCCAAATTTATAAAGAATAAATTCGTCTCCTCTTTGACCTATAATATATTGATCATTATAAGCCATGCGATTCATTCCAGACACAGCCATa < 1:43503/141‑1 (MQ=255) ttgttaCATTTCAAATTCAGTATTGTAAACACGCCGTGACTCATCTCCAAATTTATAAAGAATAAATTCGTCTCCTCTTTGACCTATAATCGATTGATCATTATAAGCCATGCGATTCATGCCAGACACAGCCATAAActc < 2:5699/141‑1 (MQ=255) | TTTGAACTACACCAACCTTTTAAATTTATTCTAGTGACAGGATAACTAAAATATATTTCTTATGCAATAATTTCCTATTGATAAGCATTTTCAGATTTTAGTTGTAAATTTTGCCCTAATTCATTTAAGTCTTGTTGCATTTCAAATTCAGTATTGTAAACACGCATTGACTCATCTCCAAATTTATAAAGAATAAATTCGTCTCCTCTTTGACCTATAATATATTGATCATTATAAGCCATGCGATTCATTCCAGACACAGCCATAAACTC > CP000730/1758746‑1759017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |