BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	522,654	C→T	T169I (ACA→ATA)	yaaT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	522,654	0	C	T	100.0%	54.8 / NA	17	T169I (ACA→ATA)	yaaT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/10); total (7/10)

TGATTTTAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTT  >  CP000730/522520‑522761

tGATTTTAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATAttttt                                                                                                       <  1:148567/141‑1 (MQ=255)

     ttAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAgggg                                                                                                  <  1:143319/141‑1 (MQ=255)

                  aaaaaTATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGt                                                                                     >  2:231255/1‑141 (MQ=255)

                         ttAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGAtt                                                                              <  2:45266/141‑1 (MQ=255)

                         ttAGAGCAACATATAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGAtt                                                                              <  2:24166/141‑1 (MQ=255)

                             cgcAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGa                                                                          >  1:172992/1‑141 (MQ=255)

                             cgcAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGAAGGTCGTTATGTTGTTCTATATGTTTAGGGGATTTTGAACCAGTATCGATTAAGa                                                                          >  2:253782/1‑141 (MQ=255)

                                 aCATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGc                                                                      <  2:250417/141‑1 (MQ=255)

                                         aaaCACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATc                                                              <  2:148927/141‑1 (MQ=255)

                                                     aGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCAt                                                  <  2:210767/141‑1 (MQ=255)

                                                          agaCAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAAt                                             >  2:66130/1‑141 (MQ=255)

                                                                  tGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTaa                                     >  1:33100/1‑141 (MQ=255)

                                                                                 aGCCAAATTGCTTGGCGGTATCGGACCTTGGGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAACATTTCTGGTGCAtg                      >  2:199649/1‑141 (MQ=255)

                                                                                           cTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTg            >  1:222141/1‑141 (MQ=255)

                                                                                           cTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTg            <  1:203708/141‑1 (MQ=255)

                                                                                                 ggTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATgtgt      <  2:287436/141‑1 (MQ=255)

                                                                                                     tCGGACCTTGTGGTAGGTCGTTATGTTGTCCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTgttgtt  <  1:284524/141‑1 (MQ=255)

TGATTTTAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTT  >  CP000730/522520‑522761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`