Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP000730	1,747,374	Δ3 bp	56.1%	coding (2073‑2075/2280 nt)	secDF ←	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	1747377 =	33 (0.330)	39 (0.420)	36/242	NT	56.1%	coding (2072/2280 nt)	secDF	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
	?	CP000730	= 1747373	30 (0.320)					coding (2076/2280 nt)	secDF	RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

CAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747524‑1747377 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAAT  <  CP000730/1747373‑1747234                                                                                                                                                                                                                                                                                                  CAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTG                                                                                                                                                     <  2:473203/141‑1    TCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAG                                                                                                                                                  <  2:495817/141‑1       TAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAG                                                                                                                                               <  1:276963/141‑1       TAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAG                                                                                                                                               <  2:634425/141‑1       TAACGTTCGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAG                                                                                                                                               <  1:595619/141‑1        AACGTTTGACCGTGTACGTGAAAACTTACCAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAAAAGTTATTGTAGTAGT                                                                                                                                              >  2:1007328/1‑141                ACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATAC                                                                                                                                      <  1:231176/141‑1                ACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATAC                                                                                                                                      >  1:179499/1‑141                 CCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACT                                                                                                                                     >  2:645760/1‑141                    TGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGATAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATT                                                                                                                                  <  2:420848/141‑1                     GTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTC                                                                                                                                 >  1:176778/1‑141                        CGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTC                                                                                                                              >  1:433210/1‑141                         GTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCG                                                                                                                             >  1:229304/1‑141                           GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGT                                                                                                                           <  2:1054298/141‑1                               ACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTC                                                                                                                       >  1:762838/1‑141                                 TTACAAAAGGTGAAAGGGATTACGACAACAGAACAAGTTGGTGATATCGTTGATAGAGCAATTAGACAGACAAGGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCa                                                                                                                     <  1:1133959/141‑2                                      AAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGAT                                                                                                                >  2:37737/1‑141                                       AAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATA                                                                                                               >  1:90926/1‑141                                          GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTC                                                                                                            >  1:154317/1‑141                                            TAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAA                                                                                                          >  2:1110477/1‑141                                              AAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAAAGAGACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACT                                                                                                        >  2:85087/1‑141                                                   ATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACT                                                                                                   >  2:1111655/1‑141                                                      ACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTA                                                                                                >  2:147681/1‑141                                                              AGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATT                                                                                        <  1:942358/141‑1                                                                 ACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTAT                                                                                     <  2:1091246/141‑1                                                                   AAATTGATGATATCGTAAATAGGCCAATTAGCCAGACAATGACACGTTCAATTAATACCGTATTGACAGGCATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCCACTTTACTTTAGCATTATTTATCG                                                                                   >  2:491842/1‑141                                                                    AATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGG                                                                                  <  1:399086/141‑1                                                                      TTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGCATCCACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGAT                                                                                <  1:622324/141‑1                                                                        GATGATATCGTTAATGGATCAATTAGACAGACACTGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATTGGATTG                                                                              <  2:889040/141‑1                                                                        GATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTG                                                                              <  1:824387/141‑1                                                                                      gAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATT                                                                <  2:22824/140‑1                                                                                        GATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCT                                                              >  2:762510/1‑141                                                                                          TCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCT                                                            <  2:394961/141‑1                                                                                           CAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTT                                                           >  2:1176428/1‑141                                                                                                  ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTT                                                    >  1:590190/1‑141                                                                                                  ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTT                                                    >  1:494778/1‑141                                                                                                       CAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTTTCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTG                                               >  2:739477/1‑141                                                                                                           GACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGT                                           <  1:9148/141‑1                                                                                                                       TAATACAGTCTTAACAGATATTGTAGTATTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTATGTTGTATTCACTTCAATCTTCATTAACGTTCCGCTATGGGG                               >  1:1050042/1‑141                                                                                                                       TAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGG                               <  1:1063222/141‑1                                                                                                                         ATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATGCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAA                             <  2:610828/141‑1                                                                                                                              GTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATG                        >  2:584412/1‑141                                                                                                                                ATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAA                      <  1:131874/141‑1                                                                                                                                   AACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGAATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAGTAATGAAAAA                   >  2:488079/1‑141                                                                                                                                     CAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAAC                 >  2:494410/1‑141                                                                                                                                             GTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTG         <  2:154317/141‑1                                                                                                                                             GTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTG         >  2:46213/1‑141                                                                                                                                              TAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGA        <  1:320017/141‑1                                                                                                                                              TAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGA        <  2:496215/141‑1                                                                                                                                                GTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA      <  2:112106/141‑1                                                                                                                                                GTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA      >  2:829782/1‑141                                                                                                                                                    TTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAAT  >  1:719238/1‑141                                                                                                                                                    TTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAAT                                                       <  2:1193698/88‑1                                                                                                                                                    TTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAAT                                                       >  1:1193698/1‑88                                                                                                                                                                                                                                                                                                  CAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747524‑1747377 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAAT  <  CP000730/1747373‑1747234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.