BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	97,167	G→T	7.3%	intergenic (‑103/+196)	USA300HOU_0095 ← / ← dus	hypothetical protein/possible tRNA‑dihydrouridine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	97,167	0	G	T	7.3%	397.2 / 3.5	123	intergenic (‑103/+196)	USA300HOU_0095/dus	hypothetical protein/possible tRNA‑dihydrouridine synthase
Reads supporting (aligned to +/- strand): ref base G (51/61); new base T (9/2); total (60/63)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCCATTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTAGAA  >  CP000730/97027‑97307

tCCATTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTg                                                                                                                                                >  1:1030465/1‑141 (MQ=255)

 ccATTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGa                                                                                                                                               >  1:1004675/1‑141 (MQ=255)

 ccATTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGa                                                                                                                                               >  1:868791/1‑141 (MQ=255)

   aTTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGAtt                                                                                                                                             >  1:802963/1‑141 (MQ=255)

      tCTTACCAAATATTCAAACCACATGTACTCAAAATGATATCTTGGTATGTACCTCCAATATTAATTTCAATAACTTTTTCTGTTTAACACTAACAGCTATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTAttcaa                                                                                                                                          <  2:187085/141‑4 (MQ=255)

        ttGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGt                                                                                                                                        >  2:542739/1‑141 (MQ=255)

        ttGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGt                                                                                                                                        >  2:762557/1‑141 (MQ=255)

        ttGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGt                                                                                                                                        >  2:77789/1‑141 (MQ=255)

         tGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTACAC‑GTTTTTTTTGGTTGGAGtt                                                                                                                                       <  2:246202/141‑1 (MQ=255)

         tGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGtt                                                                                                                                       <  2:274518/141‑1 (MQ=255)

            cAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGttttt                                                                                                                                    >  1:268516/1‑141 (MQ=255)

                tatTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCt                                                                                                                                <  1:1019976/141‑1 (MQ=255)

                tatTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCt                                                                                                                                <  2:138859/141‑1 (MQ=255)

                    cAAACCACGTGTACTCAATATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGt                                                                                                                            >  1:1176314/1‑141 (MQ=255)

                       aCCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGGTATTTTCTGAGTTaa                                                                                                                         >  1:1017889/1‑141 (MQ=255)

                          aCGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACg                                                                                                                      >  2:1166021/1‑141 (MQ=255)

                           cGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGa                                                                                                                     >  2:1052849/1‑141 (MQ=255)

                               tACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGTGTTTTTTTCTGAGTTAAACGATATc                                                                                                                 >  2:1149111/1‑141 (MQ=255)

                               tACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATc                                                                                                                 >  1:705489/1‑141 (MQ=255)

                                 cTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCt                                                                                                               >  2:785930/1‑141 (MQ=255)

                                  tCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTg                                                                                                              >  1:573171/1‑141 (MQ=255)

                                    aaaaTGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGAt                                                                                                            >  1:898391/1‑141 (MQ=255)

                                        tGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑t                                                                                                       <  1:108696/141‑1 (MQ=255)

                                           tAGCTTGGTATTGACCTCCAATAGGAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGGGTTTTAATC‑ATTTTTTTTTATTGGAGTTTTGTTCTGAGTTAAACGATATCCTGATGTA‑tttt                                                                                                    <  2:653112/141‑1 (MQ=255)

                                           tAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTTTTTTTGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑tttt                                                                                                    >  2:556785/1‑141 (MQ=255)

                                           tAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGNTATCCTGATGTA‑tttt                                                                                                    <  1:1036743/141‑1 (MQ=255)

                                            aGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑ttttt                                                                                                   <  2:26638/141‑1 (MQ=255)

                                             gCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTa                                                                                                  >  2:763670/1‑141 (MQ=255)

                                               ttGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAAt                                                                                                >  1:890170/1‑141 (MQ=255)

                                                    aTGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGc                                                                                           <  1:689545/141‑1 (MQ=255)

                                                       tACCTCCAATGGTACTTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCAcc                                                                                        <  2:438769/141‑1 (MQ=255)

                                                       tACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCAcc                                                                                        >  1:226327/1‑141 (MQ=255)

                                                        cccTCCAATAGTAATTTCAATAACTTTGTCTGTTGCACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGCAGTTCTCTTCTGCGTTAAACGATACCCTGATGTA‑TTTTTAATTTTGCACCa                                                                                       <  1:1041349/140‑1 (MQ=255)

                                                        aCCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCa                                                                                       <  2:640353/141‑1 (MQ=255)

                                                        aCCTCAAATAATAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCa                                                                                       <  1:891011/141‑1 (MQ=255)

                                                         ccTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCAt                                                                                      <  2:963048/141‑1 (MQ=255)

                                                           tCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCAttt                                                                                    >  1:795077/1‑141 (MQ=255)

                                                             cAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTcc                                                                                  >  1:1038303/1‑141 (MQ=255)

                                                             cAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTcc                                                                                  >  1:925162/1‑141 (MQ=255)

                                                              aaTAGTANTTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TGTTTAATTTTGCACCATTTCC‑a                                                                                <  2:897913/141‑1 (MQ=255)

                                                               aTAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTTTTGTAGTTTTTTTATGAGTTAAACGATATCCTGATGTA‑TTTTTAGTTTTGTACCATTTcacc                                                                                >  2:208117/1‑138 (MQ=255)

                                                                 aGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGGATATCCTGATGTT‑TTTTTAATCTTgttttattttgcatt                                                                              >  2:837217/1‑127 (MQ=255)

                                                                   tAATTTGAATCTCTTTGTCTGTTGAACACTAAGAGCAATGTTAATTTCATAATGTGTTGTAATC‑ATTTTTTTTGATTGGAGTTTTTGTCTGAGTTAAACGTTATCCTGCTGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAgg                                                                           <  2:488115/141‑1 (MQ=255)

                                                                   tAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAgg                                                                           <  1:973636/141‑1 (MQ=255)

                                                                    aaTTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTTTTTTTTTTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTTCACCATTTCC‑AAAAggt                                                                          >  2:1087117/1‑140 (MQ=255)

                                                                          aaTAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTg                                                                    >  2:1110886/1‑141 (MQ=255)

                                                                          aaTAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAAGTTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTg                                                                    >  1:573344/1‑141 (MQ=255)

                                                                              aCTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACAt                                                                <  2:1004675/141‑1 (MQ=255)

                                                                              aCTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACAt                                                                <  2:868791/141‑1 (MQ=255)

                                                                               cTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATa                                                               <  2:922595/141‑1 (MQ=255)

                                                                                 ttGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTTAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAg                                                             >  2:912423/1‑141 (MQ=255)

                                                                                  tGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAACAATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAg                                                             <  2:475386/141‑1 (MQ=255)

                                                                                  tGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGt                                                            <  1:624173/141‑1 (MQ=255)

                                                                                    tCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTaa                                                          >  2:285652/1‑141 (MQ=255)

                                                                                    tCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTaa                                                          <  1:578664/141‑1 (MQ=255)

                                                                                      tGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTaaaa                                                        <  2:717378/141‑1 (MQ=255)

                                                                                        ttGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAg                                                      >  1:806848/1‑141 (MQ=255)

                                                                                         tGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAgg                                                     >  2:176814/1‑141 (MQ=255)

                                                                                         tGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAgg                                                     <  2:1149122/141‑1 (MQ=255)

                                                                                         tGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAgg                                                     <  2:4213/141‑1 (MQ=255)

                                                                                             cacTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑A‑TTTTTTTGTTTGGAGTTTTTTTTTTAGTTTAACGATATCCTGATGTATTTTTTAATTTTACACCATTTTC‑AAAAGGATAAGTTTCATAAGTGAAAAGGcatt                                                 >  1:819372/1‑140 (MQ=255)

                                                                                                tAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCatcatc                                              <  1:460162/141‑1 (MQ=255)

                                                                                                 aaGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCg                                             <  1:137981/141‑1 (MQ=255)

                                                                                                 aaGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCg                                             >  2:955138/1‑141 (MQ=255)

                                                                                                    agCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGa                                          >  1:517344/1‑141 (MQ=255)

                                                                                                      cAATTTTAATTTCATAATGTGTTGTAAAC‑A‑TTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGtt                                       <  2:1011869/141‑1 (MQ=255)

                                                                                                       aaTTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGtt                                       <  1:704042/141‑1 (MQ=255)

                                                                                                       aaTTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGtt                                       <  2:65361/141‑1 (MQ=255)

                                                                                                        aTTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTa                                      <  1:77789/141‑1 (MQ=255)

                                                                                                         ttttAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTAt                                     >  1:1127302/1‑141 (MQ=255)

                                                                                                          tttAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATc                                    <  1:440484/141‑1 (MQ=255)

                                                                                                             aaTTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTa                                 >  1:305663/1‑141 (MQ=255)

                                                                                                               tttCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTGTTTCTTAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATc                               >  2:654055/1‑141 (MQ=255)

                                                                                                                ttCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTTATTTTGTACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGAAATAACCGGGGCGTATCCTAtaa                              >  2:534464/1‑139 (MQ=255)

                                                                                                                 tCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAg                             <  1:762557/141‑1 (MQ=255)

                                                                                                                 tCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAg                             <  2:32080/141‑1 (MQ=255)

                                                                                                                  cATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAgg                            <  1:466864/141‑1 (MQ=255)

                                                                                                                  cATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAgg                            >  1:754446/1‑141 (MQ=255)

                                                                                                                  cATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATAAgg                            >  2:1044917/1‑141 (MQ=255)

                                                                                                                   aTAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGa                           >  2:70102/1‑141 (MQ=255)

                                                                                                                   aTAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGa                           >  2:45448/1‑141 (MQ=255)

                                                                                                                      aTGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCCAAAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGaaa                         <  2:91279/141‑1 (MQ=255)

                                                                                                                        gtgtTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTAAAAAAAGAAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAAcc                      <  2:592976/141‑1 (MQ=255)

                                                                                                                          gttgtAAAC‑ATTTTTTTTGATTGGGGTTGCCGTCAGAGGGAAACGAGATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCaa                    >  2:53532/1‑141 (MQ=255)

                                                                                                                             gtAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGat                 <  2:977710/141‑1 (MQ=255)

                                                                                                                             gtAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGat                 <  2:551326/141‑1 (MQ=255)

                                                                                                                             gtAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGat                 <  1:756872/141‑1 (MQ=255)

                                                                                                                                 aC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGataata             <  1:185434/141‑1 (MQ=255)

                                                                                                                                  c‑ATTTTTTTTGATTGGAGTTTTTTTTTTTGTTAAACGATATTTTTATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATAc            >  2:917936/1‑141 (MQ=255)

                                                                                                                                    aTTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATAcc           >  1:100042/1‑141 (MQ=255)

                                                                                                                                     ttttttttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCt          <  1:1091216/141‑1 (MQ=255)

                                                                                                                                     ttttttttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCt          <  1:189388/141‑1 (MQ=255)

                                                                                                                                       ttttttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACAt                                                                >  1:831031/1‑85 (MQ=255)

                                                                                                                                       ttttttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGAAAt                                                                <  2:831031/85‑1 (MQ=255)

                                                                                                                                       ttttttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTCCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTaa        <  2:665795/141‑1 (MQ=255)

                                                                                                                                          tttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTa     <  2:802955/141‑1 (MQ=255)

                                                                                                                                          tttGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTa     <  1:870276/141‑1 (MQ=255)

                                                                                                                                          tttGATTGGAGTTTTATTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTa     <  1:991396/141‑1 (MQ=255)

                                                                                                                                             gATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTTATTTTTCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTAGaa  >  2:398228/1‑141 (MQ=255)

                                                                                                                                             gATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTAGaa  >  1:1172866/1‑141 (MQ=255)

TCCATTTCTTGCCAGATATTCAAACCACGTGTACTCAAAATGATAGCTTGGTATGTACCTCCAATAGTAATTTCAATAACTTTGTCTGTTGAACACTAAGAGCAATTTTAATTTCATAATGTGTTGTAAAC‑ATTTTTTTTGATTGGAGTTTTTTTCTGAGTTAAACGATATCCTGATGTA‑TTTTTAATTTTGCACCATTTCC‑AAAAGGATAAGTGACATAAGTAAAAAGGCATCATCGGGAGTTATCCTATCAGGAAAACCAAGATAATACCTAAGTAGAA  >  CP000730/97027‑97307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`