Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,329 T→A 9.3% N133K (AAT→AAA USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,3290TA 9.3% 693.2 / 9.7 233N133K (AAT→AAAUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (122/83);  new base A (2/26);  total (124/109)
Fisher's exact test for biased strand distribution p-value = 6.65e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

CAAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAA  >  CP000730/111189‑111469
                                                                                                                                            |                                                                                                                                             
 aaaTGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAGGACGGAGAAATACGAAGTAGCTCAGGATAATc                                                                                                                                              >  1:281203/1‑141 (MQ=255)
  aaTGGCAATTCAAAATAAAGGGGAAGCGCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAAGAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCa                                                                                                                                            <  1:27365/141‑1 (MQ=255)
   aTGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCaa                                                                                                                                           <  1:818845/141‑1 (MQ=255)
    tGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCaaa                                                                                                                                          <  1:244086/141‑1 (MQ=255)
     ggCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCaaaa                                                                                                                                         <  1:1111430/141‑1 (MQ=255)
        aaTTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCaaaaaaa                                                                                                                                      >  1:1080185/1‑141 (MQ=255)
              aaaTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATcc                                                                                                                                >  2:135251/1‑141 (MQ=255)
              aaaTAAAGGAGAAGCACAAAAAAAAAAAGAAATACATTTGAAGATAGAAAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTAAAAAAAAAAAAAAAAAATATcc                                                                                                                                <  2:772292/141‑1 (MQ=255)
                 tAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGt                                                                                                                             >  1:485251/1‑141 (MQ=255)
                 tAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGt                                                                                                                             <  1:416781/141‑1 (MQ=255)
                 tAAAGGAAAAGCTATAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAAAACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGt                                                                                                                             <  1:396996/141‑1 (MQ=255)
                  aaaGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGtt                                                                                                                            >  2:464177/1‑141 (MQ=255)
                      gagaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTaaaa                                                                                                                        >  1:677475/1‑141 (MQ=255)
                      gagaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGAAAAAAAAAAAAAAATATCCAGTTaaaa                                                                                                                        <  2:777338/141‑1 (MQ=255)
                       agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAAt                                                                                                                       >  1:102983/1‑141 (MQ=255)
                        gaAGCTCTAAAAATAAAAGGCATGCTATTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAAAAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTaaaagg                                                                                                                      >  2:228447/1‑139 (MQ=255)
                           gCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGAtt                                                                                                                   <  1:699625/141‑1 (MQ=255)
                               tAAAAATAAAAGGCATGNTTTTGAAGATNGATANAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGAAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATtaata                                                                                                               >  1:831811/1‑141 (MQ=255)
                               tAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTAAGGATAATCAAAAAAGATATCCAGTTAAAATGAGtaata                                                                                                               >  1:941270/1‑141 (MQ=255)
                                      aaaaGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAAtt                                                                                                        >  1:1014399/1‑141 (MQ=255)
                                       aaaGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                       >  1:1064134/1‑141 (MQ=255)
                                       aaaGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                       >  2:555996/1‑141 (MQ=255)
                                             aTGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCCTTTCt                                                                                                 <  2:896620/141‑1 (MQ=255)
                                             aTGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGAATAATAATAAATTCATTTca                                                                                                 >  1:205631/1‑140 (MQ=255)
                                              tccTTTTGAAGATAGATAGAAATACAAGACGTGCAAAAGGCTTCTACTAAACGAATGAAATAAGGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTa                                                                                                >  2:1004819/3‑141 (MQ=255)
                                              tGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTa                                                                                                >  2:171088/1‑141 (MQ=255)
                                                cTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACt                                                                                              <  1:266461/141‑1 (MQ=255)
                                                   ttGAAGATAGAAAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAg                                                                                           <  1:1088406/141‑1 (MQ=255)
                                                    tGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAgg                                                                                          <  2:1177236/141‑1 (MQ=255)
                                                     gAAGATAGAAAGAAATACAAGAAGAGCAAAAGGAATTTACTATACTAATGAAAAAAAGACGGAGAAATACGAAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGa                                                                                         <  2:152494/141‑1 (MQ=255)
                                                      aaGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGaa                                                                                        >  1:577242/1‑141 (MQ=255)
                                                       agatagatAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAg                                                                                       >  1:53386/1‑141 (MQ=255)
                                                        gatagataGAAATACAAGCAGTTCAAAAGGATTTTACTAAACTAAAGAAATAAAGAGGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTAATTTCTACTGAGGAAGt                                                                                      >  2:959198/1‑141 (MQ=255)
                                                             atagaAATACAAGAAGTGCAAAAGTATTTTACTATACTAATGAAATAAATACTAAGAAATACTAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaag                                                                                 <  1:410773/141‑1 (MQ=255)
                                                             atagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaag                                                                                 <  2:497655/141‑1 (MQ=255)
                                                              tagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaaga                                                                                >  1:927908/1‑141 (MQ=255)
                                                               agaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaagaa                                                                               >  2:533679/1‑141 (MQ=255)
                                                                     aCAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGAAAAAACAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAc                                                                         <  2:694201/141‑1 (MQ=255)
                                                                     aCAAGAAGTGCAAAAGGATTTTACTATACGAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAc                                                                         <  1:773766/141‑1 (MQ=255)
                                                                      cAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACa                                                                        <  1:568810/141‑1 (MQ=255)
                                                                       aagaagTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACAt                                                                       >  1:307868/1‑141 (MQ=255)
                                                                          aagTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaa                                                                    >  2:638143/1‑141 (MQ=255)
                                                                           agTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaaa                                                                   >  1:754483/1‑141 (MQ=255)
                                                                                aaaaGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGa                                                              <  2:758724/141‑1 (MQ=255)
                                                                                 aaaGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGaa                                                             >  1:239035/1‑141 (MQ=255)
                                                                                       ttttACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTAAAGAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGaa                                                       <  2:78505/141‑1 (MQ=255)
                                                                                        tttACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGaaa                                                      >  1:600561/1‑141 (MQ=255)
                                                                                         ttACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGaaaa                                                     >  2:1091220/1‑141 (MQ=255)
                                                                                         ttACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGaaaa                                                     >  1:692982/1‑141 (MQ=255)
                                                                                         ttACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGaaaa                                                     >  2:740837/1‑141 (MQ=255)
                                                                                          tactatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAAc                                                    >  2:1016515/1‑141 (MQ=255)
                                                                                          tactatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATATCTAATGTGGAGGTTAGAGTAGAAAACATAAAAAAAGAAATCAAAAAc                                                    >  1:1125536/1‑141 (MQ=255)
                                                                                           actatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACt                                                   <  1:583125/141‑1 (MQ=255)
                                                                                           actataataAAGAAATAAAGACGGAGAAATACGAAGTAGCTCAGAATAAACAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACt                                                   <  2:1103115/141‑1 (MQ=255)
                                                                                             aatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACttt                                                 <  2:729564/140‑1 (MQ=39)
                                                                                             aataaaaaaaaaaTAAAGACGGAGAAATACGAAGTAGCTCAGGATAAACAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAAAAAAAAAAAAAAAAAAAGAAATCGAAAACttt                                                 <  2:93181/131‑1 (MQ=38)
                                                                                              atactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTa                                                >  2:868805/1‑141 (MQ=255)
                                                                                                actaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGt                                             >  2:201457/1‑141 (MQ=255)
                                                                                                   aaTGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGttt                                           <  2:988866/141‑1 (MQ=255)
                                                                                                     tGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGttttt                                         <  2:71113/141‑1 (MQ=255)
                                                                                                      gAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGtttttt                                        >  1:722445/1‑141 (MQ=255)
                                                                                                      gAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGtttttt                                        >  1:658850/1‑141 (MQ=255)
                                                                                                      gAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGttttttt                                       >  1:317224/1‑140 (MQ=255)
                                                                                                        aaTAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgc                                      >  1:177521/1‑141 (MQ=255)
                                                                                                           aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                       >  1:37545/1‑73 (MQ=255)
                                                                                                           aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                       <  2:37545/73‑1 (MQ=255)
                                                                                                           aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                   >  2:1160943/1‑141 (MQ=255)
                                                                                                           aaaGACGGAGAAATACGAAGTAGCTCAGGAGAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                   >  2:1072901/1‑141 (MQ=255)
                                                                                                            aaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTGAGTTTTTTGCGCaa                                  >  2:317765/1‑141 (MQ=255)
                                                                                                            aaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                  >  2:200050/1‑141 (MQ=255)
                                                                                                            aaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                  <  2:334501/141‑1 (MQ=255)
                                                                                                               aCGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAata                                <  1:73610/141‑1 (MQ=255)
                                                                                                               aCGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAatat                               >  1:595677/1‑141 (MQ=255)
                                                                                                               aCGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAatat                               >  1:1174412/1‑141 (MQ=255)
                                                                                                                 ggAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAg                             <  2:601286/141‑1 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                            <  1:1143703/141‑1 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGATCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                            <  2:1034705/141‑1 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGTAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                         >  1:999403/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                         <  1:404844/141‑1 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                         <  1:1095136/141‑1 (MQ=255)
                                                                                                                      aaTACGAAGTAGCTCAGGATAATAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAtt                        <  2:1028112/141‑1 (MQ=255)
                                                                                                                      aaTACGAAGTAGCTCAGGATAAACAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAtt                        <  2:39980/141‑1 (MQ=255)
                                                                                                                        tACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAtttt                      >  2:823611/1‑141 (MQ=255)
                                                                                                                         aCGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTa                     <  1:1004265/141‑1 (MQ=255)
                                                                                                                         aCGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTa                     <  1:419395/141‑1 (MQ=255)
                                                                                                                         aCGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTa                     <  1:610165/141‑1 (MQ=255)
                                                                                                                          cGAAGTAGCTCAGGATAATCAAAAAAATATCCAGTTAAAAGGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTaaa                   >  1:871446/1‑141 (MQ=255)
                                                                                                                           gAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTaaa                   >  1:488581/1‑141 (MQ=255)
                                                                                                                            aaGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTATTGCGCAATATAGCAATTTTAAAg                  >  2:862548/1‑141 (MQ=255)
                                                                                                                              gTAGCAAAGGATAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGAt                <  1:429703/141‑1 (MQ=255)
                                                                                                                                 gCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTa             >  2:803127/1‑141 (MQ=255)
                                                                                                                                   tCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAAt           >  2:254085/1‑141 (MQ=255)
                                                                                                                                    cAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATg          >  2:510242/1‑141 (MQ=255)
                                                                                                                                    cAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATg          <  2:1170720/141‑1 (MQ=255)
                                                                                                                                    aaGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATg          <  1:1193016/140‑1 (MQ=255)
                                                                                                                                     aGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGa         >  2:402660/1‑141 (MQ=255)
                                                                                                                                      ggATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGaa        <  1:170688/141‑1 (MQ=255)
                                                                                                                                        aTAAACAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAAtt      <  2:941703/141‑1 (MQ=255)
                                                                                                                                          aaTCAAAAAAAATATCCAATTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTANAGATTTAATGAATtat    <  1:964703/141‑1 (MQ=255)
                                                                                                                                            tCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGATTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATaa  >  2:582058/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                             
CAAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAA  >  CP000730/111189‑111469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: