Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 119,853 C→A 57.2% G254W (GGG→TGG)  yjbB ← possible PnaS family phosphate:sodium (Na+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730119,8530CA57.2% 19.7 / 201.2 130G254W (GGG→TGG) yjbBpossible PnaS family phosphate:sodium (Na+) symporter
Reads supporting (aligned to +/- strand):  ref base C (23/32);  new base A (36/38);  total (59/71)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

Only 100 of 132 total aligned reads displayed.

TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAATTAAA  >  CP000730/119716‑119992
                                                                                                                                         |                                                                                                                                           
ttGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGAt                                                                                                                                          <  1:371803/141‑1 (MQ=255)
  gAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTa                                                                                                                                        >  1:86772/1‑141 (MQ=255)
      cAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAAACAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGtt                                                                                                                                    <  1:1013582/141‑1 (MQ=255)
        aaGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTaa                                                                                                                                  <  2:592691/141‑1 (MQ=255)
         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 >  1:507376/1‑141 (MQ=255)
         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 <  1:405877/141‑1 (MQ=255)
         aGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaaa                                                                                                                                 <  1:627206/141‑1 (MQ=255)
          gTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAg                                                                                                                                <  1:301215/141‑1 (MQ=255)
            aTTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGAt                                                                                                                              <  2:458371/141‑1 (MQ=255)
            aTTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGAt                                                                                                                              >  2:321633/1‑141 (MQ=255)
             tttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATg                                                                                                                             >  1:768825/1‑141 (MQ=255)
              ttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGa                                                                                                                            >  2:83613/1‑141 (MQ=255)
                     gTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGtt                                                                                                                                    <  1:384887/126‑1 (MQ=255)
                     gTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGtt                                                                                                                                    >  2:384887/1‑126 (MQ=255)
                         aaGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAgc                                                                                                                 >  1:598211/1‑141 (MQ=255)
                           gATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAgcgc                                                                                                               <  2:687881/141‑1 (MQ=255)
                           caTACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAgcgc                                                                                                               <  2:687465/140‑1 (MQ=255)
                             tACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGcgccg                                                                                                             >  1:1109125/1‑141 (MQ=255)
                               ccATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCa                                                                                                           <  1:474462/141‑1 (MQ=255)
                               ccATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCa                                                                                                           <  1:1151889/141‑1 (MQ=255)
                                cATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCAc                                                                                                          <  1:226473/141‑1 (MQ=255)
                                 aTGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACg                                                                                                         >  2:768117/1‑141 (MQ=255)
                                   gtgATACTGCAATCGTCATCGCTGGTTTTAACTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTAGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGtt                                                                                                       <  2:115700/141‑1 (MQ=255)
                                    tgATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGttt                                                                                                      <  2:187969/141‑1 (MQ=255)
                                         cTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                                 >  1:899203/1‑141 (MQ=255)
                                          tGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGc                                                                                                >  1:485772/1‑141 (MQ=255)
                                          tGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGc                                                                                                <  1:250527/141‑1 (MQ=255)
                                             aaTCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAt                                                                                             <  2:668330/141‑1 (MQ=255)
                                                  tCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGc                                                                                        <  1:856039/141‑1 (MQ=255)
                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                                       <  1:392660/141‑1 (MQ=255)
                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                                       >  1:329215/1‑141 (MQ=255)
                                                      cGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGc                                                                                    <  1:18192/141‑1 (MQ=255)
                                                           gTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGTAGCGATTGAGCCGGCTAAAc                                                                               <  2:834934/141‑1 (MQ=255)
                                                             tttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTa                                                                             >  1:1136482/1‑141 (MQ=255)
                                                             tttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTa                                                                             >  2:41998/1‑141 (MQ=255)
                                                             tttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATGAAGTTAAAGATGACGGGTACAAGCGCCGCACGCTTTGCAGCGATTGAGCCGGCGAAACTa                                                                             >  2:912174/1‑141 (MQ=255)
                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                                            >  1:602043/1‑141 (MQ=255)
                                                               tAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGc                                                                           >  2:951683/1‑141 (MQ=255)
                                                                aaGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCt                                                                          <  2:1109125/141‑1 (MQ=255)
                                                                 aGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTa                                                                         >  1:933263/1‑141 (MQ=255)
                                                                    gCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTCAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAgg                                                                      >  2:557892/1‑140 (MQ=255)
                                                                    gCCATAAATCTTATAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGa                                                                      <  2:829764/141‑1 (MQ=255)
                                                                          aaTCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGACAATTACCCCGATTAAGTTAAAGATGACTTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTg                                                                <  2:949897/141‑1 (MQ=255)
                                                                            tCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTa                                                              >  2:729682/1‑141 (MQ=255)
                                                                             cTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTaa                                                             <  1:1103402/141‑1 (MQ=255)
                                                                               tGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATc                                                           >  1:548557/1‑141 (MQ=255)
                                                                               tGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGGTAAAGAGGACATGTATAATCGCCGCACGTTTTGCAGCGAGTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATc                                                           >  1:596684/1‑141 (MQ=255)
                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                                 <  1:451453/102‑1 (MQ=255)
                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                                 >  2:451453/1‑102 (MQ=255)
                                                                                 tAACAAACTAATCAAATGAATCACAACTGGCAAGAANATTGTGAANATAATTANCCCGATTAAGTTAAAGATGACGTGTACAAGCGNCGCACGTTTTGCNGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGt                                                         >  1:273738/1‑141 (MQ=255)
                                                                                 tAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGt                                                         >  2:28253/1‑141 (MQ=255)
                                                                                  aaCAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTg                                                        <  1:253486/141‑1 (MQ=255)
                                                                                  aaCAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATGACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTg                                                        >  2:1010436/1‑141 (MQ=255)
                                                                                   aCAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTgg                                                       >  1:734506/1‑141 (MQ=255)
                                                                                     aaaCTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTa                                                     >  1:972603/1‑141 (MQ=255)
                                                                                         tAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCaa                                                 >  2:586068/1‑141 (MQ=255)
                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGTTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                                              <  1:632258/141‑1 (MQ=255)
                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                                              <  2:1153375/141‑1 (MQ=255)
                                                                                             cAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGtt                                             <  2:804612/141‑1 (MQ=255)
                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                                            <  1:1186339/141‑1 (MQ=255)
                                                                                                aTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATc                                          >  2:37454/1‑141 (MQ=255)
                                                                                                    aTCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCt                                      >  2:92182/1‑141 (MQ=255)
                                                                                                    aTCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCt                                      >  1:1076309/1‑141 (MQ=255)
                                                                                                     tCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTa                                     >  2:16643/1‑141 (MQ=255)
                                                                                                        caACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTa                                  >  2:325579/1‑141 (MQ=255)
                                                                                                         aaCTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTaa                                 >  2:438746/1‑141 (MQ=255)
                                                                                                          aCTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAac                                <  1:1001328/141‑1 (MQ=255)
                                                                                                             ggCAAGAAAATTGTGAAGATAATTACCCCAATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAAcaca                             <  1:666346/141‑1 (MQ=255)
                                                                                                             ggCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGNTGTNATCGTGGTACCAATNNTATCGCCTAGTAAcaca                             <  1:987478/141‑1 (MQ=255)
                                                                                                             ggCAAGAAAATCGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGAGTACAAACGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAACTGTGGTACCAATGTTATCGCCTAGTAAcaca                             >  2:1171140/1‑141 (MQ=255)
                                                                                                               cAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAgg                           >  2:1033196/1‑141 (MQ=255)
                                                                                                               cAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAgg                           <  1:1186340/141‑1 (MQ=255)
                                                                                                                aaGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAGGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAggg                          >  1:684658/1‑141 (MQ=255)
                                                                                                                 aGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGa                         <  2:181042/141‑1 (MQ=255)
                                                                                                                  gAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGAt                        <  1:703730/141‑1 (MQ=255)
                                                                                                                    aaaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGTCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtg                      >  1:537731/1‑141 (MQ=255)
                                                                                                                    aaaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtg                      >  1:1011373/1‑141 (MQ=255)
                                                                                                                     aaTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  2:627649/141‑1 (MQ=255)
                                                                                                                     aaTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  1:974402/141‑1 (MQ=255)
                                                                                                                     aaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  1:457284/141‑1 (MQ=255)
                                                                                                                     aaTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAATGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc                     <  2:301348/141‑1 (MQ=255)
                                                                                                                      aTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct                    <  2:273738/141‑1 (MQ=255)
                                                                                                                      aTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct                    <  1:445070/141‑1 (MQ=255)
                                                                                                                       ttGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgctg                   >  2:880071/1‑141 (MQ=255)
                                                                                                                          tgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGt                >  2:971321/1‑141 (MQ=255)
                                                                                                                          tgAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGt                <  2:422547/141‑1 (MQ=255)
                                                                                                                           gAAGATAATTACCCGGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGtt               <  1:153006/141‑1 (MQ=255)
                                                                                                                            aaGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGttt              >  2:1045996/1‑141 (MQ=255)
                                                                                                                            aaGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGttt              <  1:511961/141‑1 (MQ=255)
                                                                                                                             aGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTa             >  1:190692/1‑141 (MQ=255)
                                                                                                                                tAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGc          <  1:1044017/141‑1 (MQ=255)
                                                                                                                                 aaTTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCt         <  1:879333/141‑1 (MQ=255)
                                                                                                                                   ttACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTaa       <  2:318935/141‑1 (MQ=255)
                                                                                                                                   ttACCCCGATTAAGATAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTaa       <  2:349576/141‑1 (MQ=255)
                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     <  2:1065348/141‑1 (MQ=255)
                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     <  2:1122668/141‑1 (MQ=255)
                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     >  1:791941/1‑141 (MQ=255)
                                                                                                                                     aCCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATAGAGCCGGCTAAACTAGCTAAGAAAGATGTAATCGTGGCACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAAtt     <  1:1010436/141‑1 (MQ=255)
                                                                                                                                        ccGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAATTaaa  >  2:510567/1‑141 (MQ=255)
                                                                                                                                        ccGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGCTTAAGCTAATTaaa  >  1:592712/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCGTTTAAGCTAATTAAA  >  CP000730/119716‑119992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: