Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 253,983 A→G 7.9% R3R (AGA→AGG)  USA300HOU_0227 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730253,9830AG 7.9% 266.0 / 10.5 89R3R (AGA→AGG) USA300HOU_0227hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/40);  new base G (2/5);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

GAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA  >  CP000730/253842‑254121
                                                                                                                                             |                                                                                                                                          
gAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTAC‑TTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAaga                                                                                                                                            <  2:881639/141‑1 (MQ=255)
 aaTAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAaga                                                                                                                                            <  1:374534/141‑1 (MQ=255)
   tAAATGCATAAGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATAAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTCTATAGCGATGGTCATACCCATTCACAGTAACAATACTCACCATTGACAAGAGt                                                                                                                                          >  2:59247/1‑141 (MQ=255)
         aatatGTCGCCAAGGCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCCATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa                                                                                                                                    <  2:1120569/140‑1 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  1:774899/141‑4 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  1:354770/141‑4 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  2:840474/141‑4 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:216703/141‑1 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:624675/141‑1 (MQ=255)
           tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:897295/141‑1 (MQ=255)
               tCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt                                                                                                                              <  1:585871/141‑1 (MQ=255)
                cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc                                                                                                                             <  1:399652/141‑1 (MQ=255)
                cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc                                                                                                                             <  1:583259/141‑1 (MQ=255)
                  ccAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCaa                                                                                                                           <  2:1015257/141‑1 (MQ=39)
                       ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg                                                                                                                      >  2:329166/1‑141 (MQ=255)
                       ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCAATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg                                                                                                                      >  2:913871/1‑141 (MQ=255)
                        cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     <  2:863017/141‑1 (MQ=255)
                        cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     <  2:995981/141‑1 (MQ=255)
                        cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     >  2:429425/1‑141 (MQ=255)
                         aTTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAg                                                                                                                    >  2:88209/1‑141 (MQ=255)
                          ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg                                                                                                                   <  1:169700/141‑1 (MQ=255)
                          ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg                                                                                                                   <  2:924286/141‑1 (MQ=255)
                           tGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt                                                                                                                  >  1:498613/1‑141 (MQ=255)
                             ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat                                                                                                                <  1:881785/141‑1 (MQ=255)
                             ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat                                                                                                                <  1:12874/141‑1 (MQ=255)
                               aaaaaTTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTatat                                                                                                              >  2:1002931/1‑141 (MQ=255)
                                    tttCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa                                                                                                         <  1:114902/141‑1 (MQ=255)
                                       cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaataa                                                                                                      >  2:418813/1‑141 (MQ=255)
                                       cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTGTCAATAGTGAGGTATATGAtaataa                                                                                                      <  1:930164/141‑1 (MQ=255)
                                        acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATATTaaa                                                                                                     >  1:1028267/1‑141 (MQ=255)
                                        acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaa                                                                                                     >  1:1023478/1‑141 (MQ=255)
                                              tAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAg                                                                                               <  1:968865/141‑1 (MQ=255)
                                                aaTCAAGACATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGcc                                                                                             <  2:180618/141‑1 (MQ=255)
                                                   cAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgt                                                                                          >  2:1003247/1‑141 (MQ=255)
                                                      gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttgt                                                                                       >  2:859191/1‑141 (MQ=255)
                                                      gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttgt                                                                                       <  1:622918/141‑1 (MQ=255)
                                                             tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATg                                                                                >  1:454771/1‑141 (MQ=255)
                                                              ttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATAAAAAAAAAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:1037039/141‑1 (MQ=255)
                                                              gagaaaaGAAGGTGTACTTTCTATAAGTATCTAGAGATCGTCATACCCATTCAGAGAAACAAACCTCACCAATGAAAAGAGTATATAACCTTTTCGATAGGAGGGTATATGAAAAAAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:1037057/137‑1 (MQ=255)
                                                                gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGATc                                                                             >  2:40191/1‑141 (MQ=255)
                                                                 tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa                                                                            <  2:425216/141‑1 (MQ=255)
                                                                  aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAACGAGTCAATCACATTATCAATAGTAAAGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt                                                                           >  1:174634/1‑141 (MQ=255)
                                                                     gaagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGa                                                                        >  1:210804/1‑141 (MQ=255)
                                                                         gtgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg                                                                    <  1:212201/141‑1 (MQ=255)
                                                                            tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa                                                                 <  1:476149/141‑1 (MQ=255)
                                                                            tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa                                                                 <  1:51713/141‑1 (MQ=255)
                                                                                  atataAGTATATAGCGATGGTCATACCCATTCACAATAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACttt                                                           >  1:1041465/1‑141 (MQ=255)
                                                                                   tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          >  1:435974/1‑141 (MQ=255)
                                                                                   tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          <  1:191843/141‑1 (MQ=255)
                                                                                    ataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaa                                                         >  2:86469/1‑141 (MQ=255)
                                                                                       aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa                                                                                                                           <  1:534849/72‑1 (MQ=255)
                                                                                       aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa                                                                                                                           >  2:534849/1‑72 (MQ=255)
                                                                                       aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg                                                      >  1:303321/1‑141 (MQ=255)
                                                                                       aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg                                                      <  1:320364/141‑1 (MQ=255)
                                                                                            ataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCt                                                 >  1:91273/1‑141 (MQ=255)
                                                                                             taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg                                                >  2:760691/1‑141 (MQ=255)
                                                                                             taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTCTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg                                                >  1:936703/1‑141 (MQ=255)
                                                                                                 gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata                                            >  2:860093/1‑141 (MQ=255)
                                                                                                     gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataat                                        >  1:213399/1‑141 (MQ=255)
                                                                                                     gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaagataat                                        >  1:842695/1‑141 (MQ=255)
                                                                                                      tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTTTAaatatacta                                       >  1:1004918/1‑141 (MQ=255)
                                                                                                        aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATAtt                                     >  2:686513/1‑141 (MQ=255)
                                                                                                                tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:857028/141‑1 (MQ=255)
                                                                                                                tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:568373/141‑1 (MQ=255)
                                                                                                                tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  2:1125412/141‑1 (MQ=255)
                                                                                                                tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:454318/141‑1 (MQ=255)
                                                                                                                acacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGGATATAATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  2:1037141/140‑1 (MQ=255)
                                                                                                                 cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATATCAGAATGc                            >  2:298228/1‑141 (MQ=255)
                                                                                                                   caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCAc                          >  2:1107245/1‑141 (MQ=255)
                                                                                                                     gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATCGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt                        >  2:451730/1‑141 (MQ=255)
                                                                                                                       aaCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCCGTAGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTAAATATAATATTTCAGAATGCACTTTa                      >  1:416059/1‑141 (MQ=255)
                                                                                                                        aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:263325/83‑1 (MQ=255)
                                                                                                                        aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATgg                                                                               >  1:263325/1‑83 (MQ=255)
                                                                                                                        aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATCGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGACTGCACTTTaa                     >  2:1113044/1‑141 (MQ=255)
                                                                                                                           aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:874740/1‑141 (MQ=255)
                                                                                                                           aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:397964/1‑141 (MQ=255)
                                                                                                                             ccTCACCATTGAAAAGATTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATg                <  2:1180690/141‑1 (MQ=255)
                                                                                                                               tCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGa              <  2:23928/141‑1 (MQ=255)
                                                                                                                                cACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAGAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGAc             <  1:860093/141‑1 (MQ=255)
                                                                                                                                 aCCATTGAAAAGAGTATATAACCTTATCAATAGTGAGGCATATGATAATAGAAAAAAGCCCGTTGCCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATACTTCAGAATGCACTTTAAAGATGGACg            <  1:391562/141‑1 (MQ=255)
                                                                                                                                   cATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTc          <  1:1153432/141‑1 (MQ=255)
                                                                                                                                    aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg         >  1:14435/1‑141 (MQ=255)
                                                                                                                                    aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg         >  1:89742/1‑141 (MQ=255)
                                                                                                                                     ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa        <  2:803724/141‑1 (MQ=255)
                                                                                                                                     ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa        <  2:803779/141‑1 (MQ=255)
                                                                                                                                      tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGAt       >  2:1033709/1‑141 (MQ=255)
                                                                                                                                       gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:733486/1‑129 (MQ=39)
                                                                                                                                       gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  <  2:733486/129‑1 (MQ=39)
                                                                                                                                        aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt     >  2:1109983/1‑141 (MQ=255)
                                                                                                                                          aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATACTAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAg   >  1:632102/1‑141 (MQ=255)
                                                                                                                                          aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATgcag   >  1:1156103/1‑138 (MQ=255)
                                                                                                                                           agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa  <  1:785186/141‑1 (MQ=255)
                                                                                                                                           agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa  >  1:1188280/1‑141 (MQ=255)
                                                                                                                                             agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          >  2:660829/1‑83 (MQ=255)
                                                                                                                                             agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          <  1:660829/83‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GAATAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA  >  CP000730/253842‑254121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑