BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	253,984	G→T	8.5%	V4L (GTA→TTA)	USA300HOU_0227 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	253,984	0	G	T	8.5%	299.4 / 10.2	89	V4L (GTA→TTA)	USA300HOU_0227	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (42/39); new base T (2/6); total (44/45)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA  >  CP000730/253845‑254121

tAAATGCATAAGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATAAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTCTATAGCGATGGTCATACCCATTCACAGTAACAATACTCACCATTGACAAGAGt                                                                                                                                          >  2:59247/1‑141 (MQ=255)

      aatatGTCGCCAAGGCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCCATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATaa                                                                                                                                    <  2:1120569/140‑1 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  1:774899/141‑4 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  2:840474/141‑4 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGGTtatatactc                                                                                                                                  <  1:354770/141‑4 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:216703/141‑1 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:897295/141‑1 (MQ=255)

        tatGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAcc                                                                                                                                  <  1:624675/141‑1 (MQ=255)

            tCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt                                                                                                                              <  1:585871/141‑1 (MQ=255)

             cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc                                                                                                                             <  1:583259/141‑1 (MQ=255)

             cGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc                                                                                                                             <  1:399652/141‑1 (MQ=255)

               ccAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCaa                                                                                                                           <  2:1015257/141‑1 (MQ=39)

                    ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg                                                                                                                      >  2:329166/1‑141 (MQ=255)

                    ccATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCAATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTg                                                                                                                      >  2:913871/1‑141 (MQ=255)

                     cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     <  2:995981/141‑1 (MQ=255)

                     cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     >  2:429425/1‑141 (MQ=255)

                     cATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa                                                                                                                     <  2:863017/141‑1 (MQ=255)

                      aTTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAg                                                                                                                    >  2:88209/1‑141 (MQ=255)

                       ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg                                                                                                                   <  2:924286/141‑1 (MQ=255)

                       ttGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg                                                                                                                   <  1:169700/141‑1 (MQ=255)

                        tGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt                                                                                                                  >  1:498613/1‑141 (MQ=255)

                          ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat                                                                                                                <  1:881785/141‑1 (MQ=255)

                          ccAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat                                                                                                                <  1:12874/141‑1 (MQ=255)

                            aaaaaTTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTatat                                                                                                              >  2:1002931/1‑141 (MQ=255)

                                 tttCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa                                                                                                         <  1:114902/141‑1 (MQ=255)

                                    cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaataa                                                                                                      >  2:418813/1‑141 (MQ=255)

                                    cacaCCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTGTCAATAGTGAGGTATATGAtaataa                                                                                                      <  1:930164/141‑1 (MQ=255)

                                     acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATATTaaa                                                                                                     >  1:1028267/1‑141 (MQ=255)

                                     acacCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaa                                                                                                     >  1:1023478/1‑141 (MQ=255)

                                           tAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAg                                                                                               <  1:968865/141‑1 (MQ=255)

                                             aaTCAAGACATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGcc                                                                                             <  2:180618/141‑1 (MQ=255)

                                                cAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgt                                                                                          >  2:1003247/1‑141 (MQ=255)

                                                   gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttgt                                                                                       <  1:622918/141‑1 (MQ=255)

                                                   gTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttgt                                                                                       >  2:859191/1‑141 (MQ=255)

                                                          tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATg                                                                                >  1:454771/1‑141 (MQ=255)

                                                           ttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATAAAAAAAAAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:1037039/141‑1 (MQ=255)

                                                           gagaaaaGAAGGTGTACTTTCTATAAGTATCTAGAGATCGTCATACCCATTCAGAGAAACAAACCTCACCAATGAAAAGAGTATATAACCTTTTCGATAGGAGGGTATATGAAAAAAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:1037057/137‑1 (MQ=255)

                                                             gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGATc                                                                             >  2:40191/1‑141 (MQ=255)

                                                              tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa                                                                            <  2:425216/141‑1 (MQ=255)

                                                               aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAACGAGTCAATCACATTATCAATAGTAAAGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt                                                                           >  1:174634/1‑141 (MQ=255)

                                                                  gaagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGa                                                                        >  1:210804/1‑141 (MQ=255)

                                                                      gtgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg                                                                    <  1:212201/141‑1 (MQ=255)

                                                                         tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa                                                                 <  1:476149/141‑1 (MQ=255)

                                                                         tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa                                                                 <  1:51713/141‑1 (MQ=255)

                                                                               atataAGTATATAGCGATGGTCATACCCATTCACAATAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACttt                                                           >  1:1041465/1‑141 (MQ=255)

                                                                                tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          <  1:191843/141‑1 (MQ=255)

                                                                                tataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          >  1:435974/1‑141 (MQ=255)

                                                                                 ataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaa                                                         >  2:86469/1‑141 (MQ=255)

                                                                                    aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa                                                                                                                           >  2:534849/1‑72 (MQ=255)

                                                                                    aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa                                                                                                                           <  1:534849/72‑1 (MQ=255)

                                                                                    aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg                                                      >  1:303321/1‑141 (MQ=255)

                                                                                    aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAgg                                                      <  1:320364/141‑1 (MQ=255)

                                                                                         ataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCt                                                 >  1:91273/1‑141 (MQ=255)

                                                                                          taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg                                                >  2:760691/1‑141 (MQ=255)

                                                                                          taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTCTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTg                                                >  1:936703/1‑141 (MQ=255)

                                                                                              gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaata                                            >  2:860093/1‑141 (MQ=255)

                                                                                                  gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataat                                        >  1:213399/1‑141 (MQ=255)

                                                                                                  gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaagataat                                        >  1:842695/1‑141 (MQ=255)

                                                                                                   tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTTTAaatatacta                                       >  1:1004918/1‑141 (MQ=255)

                                                                                                     aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATAtt                                     >  2:686513/1‑141 (MQ=255)

                                                                                                             tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:857028/141‑1 (MQ=255)

                                                                                                             tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:454318/141‑1 (MQ=255)

                                                                                                             tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  1:568373/141‑1 (MQ=255)

                                                                                                             tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  2:1125412/141‑1 (MQ=255)

                                                                                                             acacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGGATATAATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATg                             <  2:1037141/140‑1 (MQ=255)

                                                                                                              cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATATCAGAATGc                            >  2:298228/1‑141 (MQ=255)

                                                                                                                caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCAc                          >  2:1107245/1‑141 (MQ=255)

                                                                                                                  gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATCGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACtt                        >  2:451730/1‑141 (MQ=255)

                                                                                                                    aaCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCCGTAGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTAAATATAATATTTCAGAATGCACTTTa                      >  1:416059/1‑141 (MQ=255)

                                                                                                                     aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATgg                                                                               <  2:263325/83‑1 (MQ=255)

                                                                                                                     aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATgg                                                                               >  1:263325/1‑83 (MQ=255)

                                                                                                                     aCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATCGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGACTGCACTTTaa                     >  2:1113044/1‑141 (MQ=255)

                                                                                                                        aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:874740/1‑141 (MQ=255)

                                                                                                                        aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:397964/1‑141 (MQ=255)

                                                                                                                          ccTCACCATTGAAAAGATTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATg                <  2:1180690/141‑1 (MQ=255)

                                                                                                                            tCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGa              <  2:23928/141‑1 (MQ=255)

                                                                                                                             cACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAGAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGAc             <  1:860093/141‑1 (MQ=255)

                                                                                                                              aCCATTGAAAAGAGTATATAACCTTATCAATAGTGAGGCATATGATAATAGAAAAAAGCCCGTTGCCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATACTTCAGAATGCACTTTAAAGATGGACg            <  1:391562/141‑1 (MQ=255)

                                                                                                                                cATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTc          <  1:1153432/141‑1 (MQ=255)

                                                                                                                                 aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg         >  1:14435/1‑141 (MQ=255)

                                                                                                                                 aTTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCg         >  1:89742/1‑141 (MQ=255)

                                                                                                                                  ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa        <  2:803724/141‑1 (MQ=255)

                                                                                                                                  ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGa        <  2:803779/141‑1 (MQ=255)

                                                                                                                                   tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGAt       >  2:1033709/1‑141 (MQ=255)

                                                                                                                                    gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  >  1:733486/1‑129 (MQ=39)

                                                                                                                                    gAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGa                  <  2:733486/129‑1 (MQ=39)

                                                                                                                                     aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGt     >  2:1109983/1‑141 (MQ=255)

                                                                                                                                       aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATACTAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAg   >  1:632102/1‑141 (MQ=255)

                                                                                                                                       aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATgcag   >  1:1156103/1‑138 (MQ=255)

                                                                                                                                        agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa  >  1:1188280/1‑141 (MQ=255)

                                                                                                                                        agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGa  <  1:785186/141‑1 (MQ=255)

                                                                                                                                          agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          <  1:660829/83‑1 (MQ=255)

                                                                                                                                          agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa                                                          >  2:660829/1‑83 (MQ=255)

TAAATGCATATGTCGCCAAGCCATTGCCAAAAATTTCACACCTTAAATCAAGTCATTGTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAATATTTCAGAATGCACTTTAAAGATGGACGTCGATGTAGA  >  CP000730/253845‑254121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`