BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	492,848	A→T	7.6%	F62Y (TTC→TAC)	USA300HOU_0466 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	492,848	0	A	T	7.6%	352.1 / 3.8	114	F62Y (TTC→TAC)	USA300HOU_0466	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (58/45); new base T (9/2); total (67/47)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGCGACA  >  CP000730/492710‑492988

aaGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAAc                                                                                                                                            <  1:1100368/141‑1 (MQ=255)

   ggttGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑GTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTa                                                                                                                                         >  2:41554/3‑141 (MQ=255)

   gagaggacGAATGACCTTCATGTTCTTTGTTCAACCTATCCTCATCTGAATTAGTCTTCATG‑TTTTGCTTTGCGAATGCTTGTAAATAATCTACCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTa                                                                                                                                         >  2:376136/8‑141 (MQ=255)

   gATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTa                                                                                                                                         >  2:276193/1‑141 (MQ=255)

    aTTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATGAAAGTAACAACTGCCACTGCTTTTTTGAACTTAc                                                                                                                                        >  1:1104228/1‑141 (MQ=255)

       ggTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACgtg                                                                                                                                     <  1:1037605/141‑1 (MQ=255)

         tCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACgtggt                                                                                                                                   <  2:861919/141‑1 (MQ=255)

         tCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAAGCTAGCACCGTGTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGTACTTACgtggt                                                                                                                                   <  2:874237/141‑1 (MQ=255)

         tCGAAAGAACTTCATGTATGATGTTCTAGCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTACAGAATCGAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGTACTTACgtggt                                                                                                                                   <  2:945317/141‑1 (MQ=255)

            aaTGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGAc                                                                                                                                <  2:119941/141‑1 (MQ=255)

              tGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACtt                                                                                                                              <  1:684218/141‑1 (MQ=255)

              tGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACtt                                                                                                                              >  2:780207/1‑141 (MQ=255)

              tGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACtt                                                                                                                              <  2:879829/141‑1 (MQ=255)

                aaCTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTaa                                                                                                                            <  2:66998/141‑1 (MQ=255)

                 aCTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAAt                                                                                                                           >  2:662532/1‑141 (MQ=255)

                  cTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGACACTGCTTTTTTGAACTTACGTGGTGACTTAAtt                                                                                                                          >  1:1105548/1‑141 (MQ=255)

                     cATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGaa                                                                                                                       >  1:403451/1‑141 (MQ=255)

                         ttCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAGAGTAACAACTGCCACTGCTTTTTTGATCTTACGTGGTGACTTAATTGAAATGt                                                                                                                   <  2:87648/141‑1 (MQ=255)

                         ttCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTTAACTTACGTGGTGACTTAATTGAAATGt                                                                                                                   <  2:1055067/141‑1 (MQ=255)

                           cTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTaa                                                                                                                 >  2:139334/1‑141 (MQ=255)

                            tATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTaaa                                                                                                                <  1:932387/141‑1 (MQ=255)

                            tATGTTCGACCAATCCTTTTCTGAATCAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTaaa                                                                                                                <  2:942646/141‑1 (MQ=255)

                            tATGTTCGACCAATCCTTTTCGGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTaaa                                                                                                                <  1:720799/141‑1 (MQ=255)

                              tGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAg                                                                                                              <  1:1060212/141‑1 (MQ=255)

                               gTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGt                                                                                                             >  2:1107498/1‑141 (MQ=255)

                                  cGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTa                                                                                                          >  2:755155/1‑141 (MQ=255)

                                    aCCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAac                                                                                                        >  1:44016/1‑141 (MQ=255)

                                     ccAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAaca                                                                                                       >  2:638922/1‑141 (MQ=255)

                                        aTCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACgg                                                                                                    >  2:482320/1‑141 (MQ=255)

                                         tCCTTTTCTGAATTAGTATTCATGTTTTTTTTTTTTTTTTTCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTTAACTTACGTGGTGACTTAATTGTATTGTAATAGTCTAACACgt                                                                                                    >  2:256699/1‑140 (MQ=255)

                                          ccTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGtt                                                                                                  <  2:167133/141‑1 (MQ=255)

                                             tttCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCt                                                                                               >  1:1161041/1‑141 (MQ=255)

                                             tttCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCt                                                                                               >  1:651382/1‑141 (MQ=255)

                                              ttCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTa                                                                                              >  2:285814/1‑141 (MQ=255)

                                               tatGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTAc                                                                                             >  1:5289/3‑141 (MQ=255)

                                                cTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACa                                                                                            <  2:183531/141‑1 (MQ=255)

                                                 tGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAg                                                                                           <  2:5926/141‑1 (MQ=255)

                                                   aaTTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTg                                                                                         <  1:420158/141‑1 (MQ=255)

                                                    aTTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGc                                                                                        >  1:481438/1‑141 (MQ=255)

                                                    aTTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGc                                                                                        >  1:869906/1‑141 (MQ=255)

                                                     ttAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTTTTTTTTTTTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCt                                                                                       >  2:555298/1‑141 (MQ=255)

                                                     ttAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCt                                                                                       <  1:550670/141‑1 (MQ=255)

                                                     ttAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCt                                                                                       >  2:378454/1‑141 (MQ=255)

                                                         tATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTaaa                                                                                   <  1:376953/141‑1 (MQ=255)

                                                              aTG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTaa                                                                              <  1:708169/141‑1 (MQ=255)

                                                                  ttttACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGa                                                                           >  2:177062/1‑141 (MQ=255)

                                                                    ttACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTTTTTTTTTTTTTTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGcaa                                                                         >  2:20487/1‑141 (MQ=255)

                                                                    ttACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGaaa                                                                         >  2:505663/1‑141 (MQ=255)

                                                                        tttGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGata                                                                     >  2:913233/1‑141 (MQ=255)

                                                                         ttGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGatat                                                                    >  2:466657/1‑141 (MQ=255)

                                                                          tGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGAtata                                                                   >  1:1150671/1‑141 (MQ=255)

                                                                           gCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATAc                                                                  >  1:347155/1‑141 (MQ=255)

                                                                              aaTGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTaa                                                               <  1:747231/141‑1 (MQ=255)

                                                                              aaTGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTaa                                                               <  1:174349/141‑1 (MQ=255)

                                                                                tGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAtt                                                             <  1:231055/141‑1 (MQ=255)

                                                                                tGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAtt                                                             <  2:549179/141‑1 (MQ=255)

                                                                                tGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAtt                                                             >  1:163122/1‑141 (MQ=255)

                                                                                 gCTTGTAAATAATCTGGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAttt                                                            <  1:215497/141‑1 (MQ=255)

                                                                                 acTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCATCATTGACCTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTCAAACCTAATGCCAGATATACTAAttt                                                            >  2:241408/2‑141 (MQ=255)

                                                                                  cTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAtttt                                                           <  2:1143469/141‑1 (MQ=255)

                                                                                   ttGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAAttttt                                                          <  1:1134770/141‑1 (MQ=255)

                                                                                      tAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttat                                                       >  2:1159319/1‑141 (MQ=255)

                                                                                       aaaTAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttatt                                                      >  2:286464/1‑141 (MQ=255)

                                                                                        aataatCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttattt                                                     <  2:579517/141‑1 (MQ=255)

                                                                                        aataatCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttattt                                                     <  1:819403/141‑1 (MQ=255)

                                                                                          taatCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttattttt                                                   >  1:243304/1‑141 (MQ=255)

                                                                                           aatCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATtttttattttta                                                  <  1:533081/141‑1 (MQ=255)

                                                                                            atCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAg                                                 >  1:139097/1‑141 (MQ=255)

                                                                                            atCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTCTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAACGTGTCACACGGTTTCTACAGTGATTACACCTAATGAAAGATATACTACTTTTTTATTTTTAg                                                 >  2:572551/1‑141 (MQ=255)

                                                                                                aGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATg                                             <  1:717005/141‑1 (MQ=255)

                                                                                                 gCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTTTTTTTTTTTTTTGACTTAATTGAAATGTAAAAGTTTAACACGGTTCCTACAGTGCTTAAACCTAATGAATGATATACTAATTTTTTATTTTTAGCATGa                                            >  2:1192739/1‑141 (MQ=255)

                                                                                                 gCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGa                                            >  1:87408/1‑141 (MQ=255)

                                                                                                   cccGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGata                                          <  2:294589/140‑1 (MQ=255)

                                                                                                       tttGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTta                                      <  1:857326/141‑1 (MQ=255)

                                                                                                        ttGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTtat                                     <  2:805217/141‑1 (MQ=255)

                                                                                                        ttGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTtat                                     >  2:885847/1‑141 (MQ=255)

                                                                                                        ttGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTtat                                     >  1:729961/1‑141 (MQ=255)

                                                                                                         tGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTtata                                    <  1:257369/141‑1 (MQ=255)

                                                                                                           ttattaAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGc                                  >  1:4194/1‑141 (MQ=255)

                                                                                                              ttaAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGCTCCTACAGTGCTTAAACCTAATGAAAGATACACTACTTTTTTATTCTTAGCATGATATTTATAGCCAt                               >  1:261345/1‑141 (MQ=255)

                                                                                                                aaaGTAACAACTGCCACTGCTTTTTTTTTTTTTTTTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTg                             >  2:74569/1‑141 (MQ=255)

                                                                                                                      acaacTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAggat                       >  2:878300/1‑138 (MQ=255)

                                                                                                                       caacTGCCACTGCTTTTTTTAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGt                      >  2:921608/1‑141 (MQ=255)

                                                                                                                       caacTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGt                      >  1:958491/1‑141 (MQ=255)

                                                                                                                       caacTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGt                      >  1:1112711/1‑141 (MQ=255)

                                                                                                                       caacTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGt                      >  1:824946/1‑141 (MQ=255)

                                                                                                                         acTGCCACTGCTTTTTTTTTTTTTTTTTGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAACGATATACTAATTTTTTATTTTTAGCATGATTTTTTTAGTTTTTGTAGCCGTct                    >  2:44210/1‑140 (MQ=255)

                                                                                                                         acTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCg                    <  2:683983/141‑1 (MQ=255)

                                                                                                                            gCCACTGCTTTTTTTTTTTTTTTTGGTGACTTAATTGAAATGTAAAAGTTTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACt                 >  2:932076/1‑141 (MQ=255)

                                                                                                                            gCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACt                 >  2:932169/1‑141 (MQ=255)

                                                                                                                            gCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACt                 >  2:418592/1‑141 (MQ=255)

                                                                                                                            gCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACt                 <  1:638922/141‑1 (MQ=255)

                                                                                                                             ccACTGCTTTTTTTTTTTTTTTTGGTGACTAAATTGAAATGTAAAAGTTTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGTTATTTTTAGCCATTGTAGCCGTCGACTa                >  2:1044285/1‑141 (MQ=255)

                                                                                                                                 tGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGaa            >  1:128770/1‑141 (MQ=255)

                                                                                                                                    ttttttGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAAcc         <  2:381799/141‑1 (MQ=255)

                                                                                                                                      ttttGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTg       <  1:177062/141‑1 (MQ=255)

                                                                                                                                       tttGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGc      >  1:399659/1‑141 (MQ=255)

                                                                                                                                       tttGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGc      >  1:399270/1‑141 (MQ=255)

                                                                                                                                          gAACTTACGTGGTGACTTAATTGAAATGTAAAAGCCTAACACGGTTCCTACAGTGCTTCAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGCAAc   >  1:547095/1‑141 (MQ=255)

                                                                                                                                           aaCTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGCGACa  <  2:139097/141‑1 (MQ=255)

AAGGATTGGTCGAATGAACTTCATGTTCTATGTTCGACCAATCCTTTTCTGAATTAGTATTCATG‑TTTTACTTTGCGAATGCTTGTAAATAATCTAGCACCGTTTGTTATTAAAGTAACAACTGCCACTGCTTTTTTGAACTTACGTGGTGACTTAATTGAAATGTAAAAGTCTAACACGGTTCCTACAGTGCTTAAACCTAATGAAAGATATACTAATTTTTTATTTTTAGCATGATATTTATAGCCATTGTAGCCGTCGACTATGAAACCTGCGACA  >  CP000730/492710‑492988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`