BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	937,158	T→A	6.4%	intergenic (+65/‑1952)	yumB → / → USA300HOU_0903	NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	937,158	0	T	A	6.4%	297.8 / 9.0	93	intergenic (+65/‑1952)	yumB/USA300HOU_0903	NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand): ref base T (40/47); new base A (3/3); total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 101 total aligned reads displayed.

GAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAT  >  CP000730/937018‑937298

gAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAgtgt                                                                                                                                              >  2:1143957/1‑141 (MQ=255)

 aaaaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTc                                                                                                                                             >  1:134059/1‑141 (MQ=255)

  aaaaaGTGATTGGTACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCa                                                                                                                                            <  2:1161478/141‑1 (MQ=255)

   aaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAc                                                                                                                                           >  2:915789/1‑141 (MQ=255)

    aaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                                          <  1:1854/141‑1 (MQ=255)

      aGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGa                                                                                                                                        <  2:867731/141‑1 (MQ=255)

       gTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGaa                                                                                                                                       >  2:849689/1‑141 (MQ=255)

         gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                     <  1:935851/141‑1 (MQ=255)

         gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                     <  1:117616/141‑1 (MQ=255)

         gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAgg                                                                                                                                     <  1:129106/141‑1 (MQ=255)

         gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTATAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                     >  2:1029118/1‑141 (MQ=255)

          attgatCCACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGAATTAAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAggg                                                                                                                                    <  2:642706/141‑1 (MQ=255)

          attgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAgga                                                                                                                                    <  2:953878/141‑2 (MQ=255)

           ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                   <  1:456573/141‑1 (MQ=255)

           ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                   <  2:584162/141‑1 (MQ=255)

              atACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCttt                                                                                                                                >  1:996767/1‑141 (MQ=255)

                       cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       >  1:761897/1‑141 (MQ=255)

                       cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       <  1:215428/141‑1 (MQ=255)

                         gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                     <  1:933343/141‑1 (MQ=255)

                           aTTCAAAATCGGTGGTATCGGTTTAGCATTCAANAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGNACAAt                                                                                                                   >  1:31315/1‑141 (MQ=255)

                                aaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGaa                                                                                                              <  2:728567/141‑1 (MQ=255)

                                aaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGaa                                                                                                              <  2:845410/141‑1 (MQ=255)

                                     ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                         >  1:355618/1‑141 (MQ=255)

                                     ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                         <  1:1190893/141‑1 (MQ=255)

                                        ggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTaa                                                                                                      >  2:394731/1‑141 (MQ=255)

                                        ggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTaa                                                                                                      <  1:989972/141‑1 (MQ=255)

                                         gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                     <  2:1112068/141‑1 (MQ=255)

                                            tCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACata                                                                                                  <  1:24062/141‑1 (MQ=255)

                                             cGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACatat                                                                                                 <  2:711761/141‑1 (MQ=255)

                                              ggTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATc                                                                                                <  2:933716/141‑1 (MQ=255)

                                               gTTTAGCAATCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCa                                                                                               >  1:488590/1‑141 (MQ=255)

                                                 ttAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCaaa                                                                                             <  2:805326/141‑1 (MQ=255)

                                                 ttAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCaaa                                                                                             <  1:517922/141‑1 (MQ=255)

                                                 ttAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCaaa                                                                                             <  2:603695/141‑1 (MQ=255)

                                                    gaaTTCAAAAAAGGTAAAGTATAGGCTTTAAATAAAGTATCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCGTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCt                                                                                          <  2:509514/139‑1 (MQ=255)

                                                    gCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCt                                                                                          >  2:128870/1‑141 (MQ=255)

                                                     cATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTa                                                                                         >  1:516476/1‑141 (MQ=255)

                                                      aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                        <  1:755303/141‑1 (MQ=255)

                                                      aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                        <  1:269937/141‑1 (MQ=255)

                                                        tCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAagg                                                                                      >  1:820358/1‑141 (MQ=255)

                                                              aaGGTAAATTCTAGGCTGTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTAc                                                                                >  2:546536/1‑141 (MQ=255)

                                                              aaGGGAAATGCTATGCAGGAAATAAAGTTGCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTTAGTAACATATCAAATCTAAGGAGGTAc                                                                                <  2:772917/141‑1 (MQ=255)

                                                               aGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACa                                                                               >  1:342392/1‑141 (MQ=255)

                                                                ggTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAt                                                                              >  1:792038/1‑141 (MQ=255)

                                                                ggTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAt                                                                              >  2:906459/1‑141 (MQ=255)

                                                                 gTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAtt                                                                             <  1:982642/141‑1 (MQ=255)

                                                                   aaaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTAt                                                                           <  1:873173/141‑1 (MQ=255)

                                                                    aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                          <  1:599202/141‑1 (MQ=255)

                                                                    aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                          <  1:779416/141‑1 (MQ=255)

                                                                    aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGATTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                          >  2:103849/1‑141 (MQ=255)

                                                                     aTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGa                                                                         >  2:472701/1‑141 (MQ=255)

                                                                        cTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAtt                                                                      <  2:573518/141‑1 (MQ=255)

                                                                          aGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAtttt                                                                    >  1:4725/1‑141 (MQ=255)

                                                                            gCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaa                                                                  >  1:544346/1‑141 (MQ=255)

                                                                             cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                                 <  2:552133/141‑1 (MQ=255)

                                                                                        gTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacac                                                      >  1:784902/1‑141 (MQ=255)

                                                                                          ttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacacac                                                    <  1:124442/141‑1 (MQ=255)

                                                                                           tCAGCTAAAAACAAACAACTAAATTATGCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATAAAATAACACACt                                                   >  2:1193910/1‑141 (MQ=255)

                                                                                            cAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTa                                                  >  2:175135/1‑141 (MQ=255)

                                                                                             atatAAAAACATAAAATTGAATTATTCATTTATAAAGTACTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACCTTATGAATTTtaaattaaattaaccaccaa                                                 >  2:186594/4‑131 (MQ=255)

                                                                                              gCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAg                                                <  2:964766/141‑1 (MQ=255)

                                                                                                tAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCa                                              <  2:323335/141‑1 (MQ=255)

                                                                                                tAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCa                                              <  2:552410/141‑1 (MQ=255)

                                                                                                 aaaCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCaa                                             <  2:955182/141‑1 (MQ=255)

                                                                                                   acacATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACg                                           <  1:397643/141‑1 (MQ=255)

                                                                                                    cacaTAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGa                                          >  2:4176/1‑141 (MQ=255)

                                                                                                       aTAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaat                                       >  2:94727/1‑141 (MQ=255)

                                                                                                        tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaata                                      >  2:81424/1‑141 (MQ=255)

                                                                                                           aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGAACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaat                                   >  2:1147958/1‑141 (MQ=255)

                                                                                                             ttaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATAAAATAACACACTAATCAACGAAATAAATAc                                 >  1:427163/1‑141 (MQ=255)

                                                                                                              caaattgtacaacaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACa                                >  2:568606/15‑141 (MQ=37)

                                                                                                               aaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACAt                               <  2:924336/141‑1 (MQ=255)

                                                                                                                 attattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTAGAAGTGAGTGACATATCAAATCTAAGGAGGTACATAATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTg                             >  2:1100543/1‑141 (MQ=255)

                                                                                                                  ttattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                            >  2:55516/1‑141 (MQ=255)

                                                                                                                     ttCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGatta                         <  2:820358/141‑1 (MQ=255)

                                                                                                                     ttCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGatta                         <  2:548722/141‑1 (MQ=255)

                                                                                                                         tttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                     <  2:1041144/141‑1 (MQ=255)

                                                                                                                            ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                  <  2:264960/141‑1 (MQ=255)

                                                                                                                            ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                  <  2:935308/141‑1 (MQ=255)

                                                                                                                               aaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc               >  2:177396/1‑141 (MQ=255)

                                                                                                                                 gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg             >  2:412114/1‑141 (MQ=255)

                                                                                                                                 gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg             >  1:849853/1‑141 (MQ=255)

                                                                                                                                  ctcTTCAGGGACAGTGAGGGACTTTATTGTTTTATTATTTGATATTAGGAACATATTAAATCTAATGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACTAAGGAAAGAGAGTAATTAGTGGTATACCTGa            >  2:1009299/3‑141 (MQ=255)

                                                                                                                                   ccTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGaa           <  2:809594/141‑1 (MQ=255)

                                                                                                                                   ccTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGaa           >  1:1115865/1‑141 (MQ=255)

                                                                                                                                     ttCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACa         <  1:593431/141‑1 (MQ=255)

                                                                                                                                     ttCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACa         <  1:695065/141‑1 (MQ=255)

                                                                                                                                      tCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAt        >  1:337527/1‑141 (MQ=255)

                                                                                                                                      tCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAt        >  1:402565/1‑141 (MQ=255)

                                                                                                                                      tCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACAATAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAt        >  1:241770/1‑141 (MQ=255)

                                                                                                                                       cAGTTTCACTTAAGTGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAATGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAtt       <  2:652808/141‑1 (MQ=255)

                                                                                                                                       cAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAtt       >  1:926780/1‑141 (MQ=255)

                                                                                                                                        aGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAttt      >  1:576308/1‑141 (MQ=255)

                                                                                                                                         gtgtCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTCCATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTa     >  2:201169/1‑141 (MQ=255)

                                                                                                                                         gtgtCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTa     <  1:800208/141‑1 (MQ=255)

                                                                                                                                          tgtCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTaa    >  1:449184/1‑141 (MQ=255)

                                                                                                                                            tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                            >  1:614372/1‑115 (MQ=255)

                                                                                                                                            tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                            <  2:614372/115‑1 (MQ=255)

                                                                                                                                            tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAt  <  2:223678/141‑1 (MQ=255)

                                                                                                                                            tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAAGTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAt  <  1:998041/141‑1 (MQ=255)

GAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAT  >  CP000730/937018‑937298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`